公开(公告)号：US20130130929A1

公开(公告)日：2013-05-23

申请号：US13681251

申请日：2012-11-19

申请人： Bhairavi PARIKH ,  Michael D. Brody ,  James Stone ,  Brian Awabdy ,  Urvi Ved ,  Anh Tran ,  Patrick J. Collins ,  David J. Anvar

发明人： Bhairavi PARIKH ,  Michael D. Brody ,  James Stone ,  Brian Awabdy ,  Urvi Ved ,  Anh Tran ,  Patrick J. Collins ,  David J. Anvar

IPC分类号： C12Q1/68

CPC分类号： G01N33/5005 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6883 ,  G01N33/689

摘要： A method for concentrating and isolating nucleated cells, such as maternal and fetal nucleated red blood cells (nRBCs), in a maternal whole blood sample. The invention also provides methods and apparatus for preparing to analyze and analyzing the sample for identification of fetal genetic material as part of prenatal genetic testing. The invention also pertains to methods and apparatus for discriminating fetal nucleated red blood cells from maternal nucleated red blood cells obtained from a blood sample taken from a pregnant woman.

摘要翻译： 在母体全血样品中浓缩和分离有核细胞如母体和胎儿有核红细胞（nRBC）的方法。 本发明还提供了用于分析和分析用于鉴定胎儿遗传物质的样品作为产前遗传测试的一部分的方法和装置。 本发明还涉及从从孕妇采集的血液样品获得的母体成核红细胞中区分胎儿有核红细胞的方法和装置。

公开(公告)号：US07029854B2

公开(公告)日：2006-04-18

申请号：US10303151

申请日：2002-11-22

申请人： Patrick J. Collins ,  Keith C. Butler ,  Peter G. Webb ,  Karen W. Shannon ,  Sandra L. Tang

发明人： Patrick J. Collins ,  Keith C. Butler ,  Peter G. Webb ,  Karen W. Shannon ,  Sandra L. Tang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  G06F19/22

摘要： Methods of identifying a sequence of a nucleic acid that is suitable for use as a surface immobilized probe for two or more mRNA transcripts encoded by the same gene are provided. In practicing the subject methods, a consensus region for the two or more transcripts is first identified, and this identified consensus region is then employed to identify the suitable nucleic acid sequence, e.g., by using a probe design protocol. The subject invention also includes algorithms for performing the subject methods recorded on a computer readable medium, as well as computational analysis systems that include the same. Also provided are nucleic acid arrays produced with probes having sequences identified by the subject methods, as well as methods for using the same.

摘要翻译： 提供了鉴定适合用作由相同基因编码的两个或更多个mRNA转录物的表面固定化探针的核酸序列的方法。 在实施本发明方法时，首先鉴定两个或多个转录物的共有区域，然后使用该鉴定的共有区域来鉴定合适的核酸序列，例如通过使用探针设计方案。 本发明还包括用于执行记录在计算机可读介质上的主题方法的算法，以及包括其的计算分析系统。 还提供了由具有由本发明方法鉴定的序列的探针产生的核酸阵列，以及使用该序列的方法。

公开(公告)号：US07302348B2

公开(公告)日：2007-11-27

申请号：US10859868

申请日：2004-06-02

申请人： Jayati Ghosh ,  Bill J. Peck ,  Eric M. Leproust ,  Charles David Troup ,  Glenda Choate Delenstarr ,  Patrick J. Collins ,  John F. Corson ,  Paul K. Wolber ,  Xiangyang Zhou

发明人： Jayati Ghosh ,  Bill J. Peck ,  Eric M. Leproust ,  Charles David Troup ,  Glenda Choate Delenstarr ,  Patrick J. Collins ,  John F. Corson ,  Paul K. Wolber ,  Xiangyang Zhou

IPC分类号： G06F19/00 ,  G06F15/00 ,  G11C17/00

CPC分类号： G06F19/24 ,  G06F19/20

摘要： A method and system for quantifying and correcting spatial-intensity trends for each channel of a microarray data set having one or more channels. The method and system of one embodiment of the present invention selects a set of features from each channel of the microarray data set. Based on the selected set of features, a surface is used to determine the intensities for all features in each channel of the microarray data set. Spatial-intensity trends within the microarray data set are quantified, based on the surface to the intensities for each channel of the microarray data set. After the surface has been determined, the spatial-intensity trend can be removed from the microarray data set.

摘要翻译： 一种用于量化和校正具有一个或多个通道的微阵列数据集的每个通道的空间强度趋势的方法和系统。 本发明的一个实施例的方法和系统从微阵列数据集的每个通道中选择一组特征。 基于所选择的特征集合，使用表面来确定微阵列数据集的每个通道中的所有特征的强度。 基于微阵列数据集的每个通道的表面到强度，量化微阵列数据集内的空间强度趋势。 在确定表面后，可以从微阵列数据集中去除空间强度趋势。

公开(公告)号：US20080207960A1

公开(公告)日：2008-08-28

申请号：US11713377

申请日：2007-02-28

申请人： Eric Lin ,  Eric M. Leproust ,  Patrick J. Collins

发明人： Eric Lin ,  Eric M. Leproust ,  Patrick J. Collins

IPC分类号： C07H21/04 ,  C07C19/08 ,  C07F7/02

CPC分类号： C07H21/04 ,  C07C1/26 ,  C07C17/10 ,  C07C2529/40 ,  C07C9/00 ,  C07C19/075

摘要： In some embodiments, the present disclosure relates to systems, compositions, and methods for treating a microarray. The compositions include a superwetting agent. The methods include contacting the microarray with an aqueous mixture including the superwetting agent after a hybridization step. Kits for carrying out the methods are also provided.

摘要翻译： 在一些实施方案中，本公开涉及用于治疗微阵列的系统，组合物和方法。 组合物包括超润湿剂。 所述方法包括在杂交步骤之后使微阵列与包含超润湿剂的含水混合物接触。 还提供了实施方法的工具包。

公开(公告)号：US20060008836A1

公开(公告)日：2006-01-12

申请号：US11193107

申请日：2005-07-29

申请人： Douglas A. Amorese ,  Karen W. Shannon ,  Patrick J. Collins ,  Paul K. Wolber

发明人： Douglas A. Amorese ,  Karen W. Shannon ,  Patrick J. Collins ,  Paul K. Wolber

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00707 ,  C40B50/14

摘要： A polynucleotide array, and methods of making and using such arrays. The array may include a first set of multiple features each of which has first polynucleotide molecules of at least 400 nucleotides in length, and a second set of features each of which has second polynucleotide molecules of no more than 100 nucleotides in length. The second set of features can be used as control features, or to replace failed sequences in an enzymatic amplification to produce first polynucleotides, or to detect polymorphisms or splice variants which may not be detected by a particular first polynucleotide.

公开(公告)号：US06753145B2

公开(公告)日：2004-06-22

申请号：US09900084

申请日：2001-07-05

申请人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

发明人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

IPC分类号： C12Q168

CPC分类号： C12Q1/689

摘要： A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize nucleic acid targets, while preserving theintactness of the adsorbed polymer surface of the array. The buffer composition comprises a non-chelating buffering agent, a pH within a range of pH 6.4 and 7.5, a monovalent cation having a monovalent cation concentration that ranges from about 0.01 M to about 2.0 M, and optionally relatively lower concentrations of a chelating agent and an ionic surfactant. The total cation concentration of the buffer composition ranges from about 0.02 M to about 2.0 M. The method comprises incubating the targets with the microarray in the buffer composition at a temperature between about 55° C. and 70° C.

摘要翻译： 用于杂交与硅质底物的吸附的聚合物表面结合的核酸的微阵列的缓冲组合物，方法和试剂盒提供了与核酸靶标杂交的条件的包络线，同时保持阵列的吸附的聚合物表面的活性。 缓冲剂组合物包含非螯合缓冲剂，pH在6.4和7.5范围内的pH，一价阳离子浓度范围为约0.01M至约2.0M，任选相对较低浓度的螯合剂 和离子表面活性剂。 缓冲剂组合物的总阳离子浓度范围为约0.02M至约2.0M。该方法包括在约55℃至70℃的温度下将靶标与缓冲液组合物中的微阵列温育。