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6 条记录 (耗时 0.039 秒)

    Methods designing multiple mRNA transcript nucleic acid probe sequences for use in nucleic acid arrays
    1.
    发明授权
    Methods designing multiple mRNA transcript nucleic acid probe sequences for use in nucleic acid arrays 失效
    设计用于核酸阵列的多个mRNA转录核酸探针序列的方法

    公开(公告)号：US07029854B2

    公开(公告)日：2006-04-18

    申请号：US10303151

    申请日：2002-11-22

    申请人： Patrick J. Collins ,  Keith C. Butler ,  Peter G. Webb ,  Karen W. Shannon ,  Sandra L. Tang

    发明人： Patrick J. Collins ,  Keith C. Butler ,  Peter G. Webb ,  Karen W. Shannon ,  Sandra L. Tang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  G06F19/22

    摘要： Methods of identifying a sequence of a nucleic acid that is suitable for use as a surface immobilized probe for two or more mRNA transcripts encoded by the same gene are provided. In practicing the subject methods, a consensus region for the two or more transcripts is first identified, and this identified consensus region is then employed to identify the suitable nucleic acid sequence, e.g., by using a probe design protocol. The subject invention also includes algorithms for performing the subject methods recorded on a computer readable medium, as well as computational analysis systems that include the same. Also provided are nucleic acid arrays produced with probes having sequences identified by the subject methods, as well as methods for using the same.

    摘要翻译： 提供了鉴定适合用作由相同基因编码的两个或更多个mRNA转录物的表面固定化探针的核酸序列的方法。 在实施本发明方法时，首先鉴定两个或多个转录物的共有区域，然后使用该鉴定的共有区域来鉴定合适的核酸序列，例如通过使用探针设计方案。 本发明还包括用于执行记录在计算机可读介质上的主题方法的算法，以及包括其的计算分析系统。 还提供了由具有由本发明方法鉴定的序列的探针产生的核酸阵列，以及使用该序列的方法。

    Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
    3.
    发明授权
    Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces 失效
    缓冲液组合物和微阵列在吸附聚合物硅质表面上的杂交方法

    公开(公告)号：US06753145B2

    公开(公告)日：2004-06-22

    申请号：US09900084

    申请日：2001-07-05

    申请人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

    发明人： Nelson R. Holcomb ,  Patrick J. Collins ,  Karen W. Shannon ,  Steven M. Lefkowitz

    IPC分类号： C12Q168

    CPC分类号： C12Q1/689

    摘要： A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize nucleic acid targets, while preserving theintactness of the adsorbed polymer surface of the array. The buffer composition comprises a non-chelating buffering agent, a pH within a range of pH 6.4 and 7.5, a monovalent cation having a monovalent cation concentration that ranges from about 0.01 M to about 2.0 M, and optionally relatively lower concentrations of a chelating agent and an ionic surfactant. The total cation concentration of the buffer composition ranges from about 0.02 M to about 2.0 M. The method comprises incubating the targets with the microarray in the buffer composition at a temperature between about 55° C. and 70° C.

    摘要翻译： 用于杂交与硅质底物的吸附的聚合物表面结合的核酸的微阵列的缓冲组合物，方法和试剂盒提供了与核酸靶标杂交的条件的包络线，同时保持阵列的吸附的聚合物表面的活性。 缓冲剂组合物包含非螯合缓冲剂，pH在6.4和7.5范围内的pH，一价阳离子浓度范围为约0.01M至约2.0M，任选相对较低浓度的螯合剂 和离子表面活性剂。 缓冲剂组合物的总阳离子浓度范围为约0.02M至约2.0M。该方法包括在约55℃至70℃的温度下将靶标与缓冲液组合物中的微阵列温育。