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    Anti-viral guanosine-rich tetrad forming oligonucleotides
    5.
    发明授权
    Anti-viral guanosine-rich tetrad forming oligonucleotides 失效
    抗病毒鸟苷酸富含四肽形成寡核苷酸

    公开(公告)号：US06288042B1

    公开(公告)日：2001-09-11

    申请号：US09017974

    申请日：1998-02-03

    申请人： Robert F. Rando ,  Joshua O. Ojwaug ,  Michael E. Hogan ,  Thomas L. Wallace ,  Paul A. Cossum

    发明人： Robert F. Rando ,  Joshua O. Ojwaug ,  Michael E. Hogan ,  Thomas L. Wallace ,  Paul A. Cossum

    IPC分类号： A61K3170

    CPC分类号： A61K31/70 ,  A61K38/00 ,  C07H21/00 ,  C12N15/1132 ,  C12N2310/151 ,  C12N2310/18 ,  C12N2310/315 ,  C12N2310/335

    摘要： Guanosine-rich oligonucleotides having sequences that favor the formation under physiological conditions of a stable four-stranded structure containing two stacked guanosine quartets (G4s) are disclosed. These oligonucleotides demonstrate enhanced nuclease resistance, cellular uptake and biological efficacy. Methods and composition for treating viral infection using these guanosine-rich oligonucleotides are also disclosed. Certain embodiments of the new oligonucleotides are 16-17 nucleotides long and contain at least one C-5 propynyl dU substitution. A method for designing anti-viral oligonucleotides is also disclosed.

    摘要翻译： 公开了具有有利于在包含两个堆叠鸟苷四重奏（G4）的稳定的四链结构的生理条件下形成的序列的富含鸟苷酸的寡核苷酸。 这些寡核苷酸表现出增强的核酸酶抗性，细胞摄取和生物功效。 还公开了使用这些富含鸟嘌呤的寡核苷酸治疗病毒感染的方法和组合物。 新的寡核苷酸的某些实施方案长16-17个核苷酸并且含有至少一个C-5丙炔基dU取代。 还公开了设计抗病毒寡核苷酸的方法。

    Multiplex Viral Pathogen Analysis and Uses Thereof
    7.
    发明申请
    Multiplex Viral Pathogen Analysis and Uses Thereof 有权

    公开(公告)号：US20220298547A1

    公开(公告)日：2022-09-22

    申请号：US17698400

    申请日：2022-03-18

    申请人： Michael E. Hogan

    发明人： Michael E. Hogan

    IPC分类号： C12Q1/686 ,  C12Q1/70 ,  C12Q1/689 ,  C12Q1/6853 ,  C12Q1/6874

    摘要： Provided herein are methods for isolating, detecting and analyzing a virus in a sample. Generally, the virus is isolated via centrifuging, incubating, aggregating, and lysing the sample to obtain a crude neutralized lysate. The crude neutralized lysate is analyzed and the virus(es) are detected by performing one of a first polymerase chain reaction (PCR) amplification followed by a second PCR amplification, by an isothermal amplification or by the reverse transcription reaction and the first polymerase chain reaction amplification together utilizing fluorescently labeled primer pairs and hybridizing the resultant fluorescent labeled amplicons to complementary nucleic acid probes.

    Population scale HLA-typing and uses thereof
    9.
    发明授权
    Population scale HLA-typing and uses thereof 有权
    人口规模HLA型分型及其用途

    公开(公告)号：US07667026B2

    公开(公告)日：2010-02-23

    申请号：US11711561

    申请日：2007-02-27

    申请人： Michael E. Hogan ,  Krishna Jayaraman ,  Rahul Mitra ,  Frederick H. Eggers

    发明人： Michael E. Hogan ,  Krishna Jayaraman ,  Rahul Mitra ,  Frederick H. Eggers

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q1/6881 ,  C12Q2600/156

    摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.

    摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。