公开(公告)号：US5672481A

公开(公告)日：1997-09-30

申请号：US052784

申请日：1993-04-23

申请人： Billy W. Minshall ,  Patrick M. Maloney ,  Fred Mill ,  Shelly Heimfeld ,  Stanley Corpuz ,  Penny Thompson ,  Eric Peterson

发明人： Billy W. Minshall ,  Patrick M. Maloney ,  Fred Mill ,  Shelly Heimfeld ,  Stanley Corpuz ,  Penny Thompson ,  Eric Peterson

IPC分类号： G01N33/48 ,  A61M1/02 ,  A61M1/36 ,  B01D61/14 ,  B01D61/18 ,  B01D61/20 ,  B01D63/02 ,  B01F13/08 ,  B01F15/00 ,  B01L3/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/36 ,  G01N1/10 ,  G01N1/36 ,  G01N11/00 ,  G01N33/49 ,  G01N33/50 ,  G01N35/04 ,  G01N35/10 ,  G01N33/543

CPC分类号： B01D61/147 ,  A61M1/0281 ,  A61M1/3692 ,  B01D61/18 ,  B01D61/20 ,  B01F13/08 ,  B01F13/0818 ,  B01L3/50853 ,  C12M47/02 ,  C12M47/12 ,  G01N33/491 ,  G01N33/5002 ,  A61M1/0272 ,  B01F15/00123 ,  G01N2001/1081 ,  G01N2011/0053 ,  G01N2035/1039 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255

摘要： A particle separator is provided for collection and manipulation of target particles, e.g., target cells, in a closed sterile field condition. In one embodiment, closed sterile field conditions are maintained from separation through concentration and/or cryo treatment steps and/or transfusion. Preservation of closed sterile field condition are accommodated by using the same integrally coupled rigid-walled vessel for collection and concentration and transfer via integrally coupled conduits to a vessel for cryopreservation and/or transfusion. In one embodiment, a plurality of valves are responsive to a data processor for controlling the path of fluid flow through the particle separator. A plurality of sensors are provided for providing sensor signals indicative of fluid flowing through the cell separator. A peristaltic pump is responsive to the microprocessor assembly for controlling the speed and direction of fluid flow through the system. A stir plate assembly is responsive to a signal from the data processor for controllably agitating the contents of the cell separator.

摘要翻译： 提供颗粒分离器用于在封闭的无菌田间条件下收集和操纵靶颗粒，例如靶细胞。 在一个实施方案中，通过浓缩和/或低温治疗步骤和/或输血分离来保持封闭的无菌田间条件。 通过使用相同的整体耦合的刚性壁容器来保存封闭的无菌田间条件，用于通过整体耦合的管道进行收集和浓缩和转运至用于冷冻保存和/或输血的容器。 在一个实施例中，多个阀响应于数据处理器来控制通过颗粒分离器的流体流动的路径。 提供多个传感器用于提供指示流过细胞分离器的流体的传感器信号。 蠕动泵响应于微处理器组件来控制通过系统的流体流动的速度和方向。 搅拌板组件响应于来自数据处理器的信号，用于可控地搅动细胞分离器的内容物。

公开(公告)号：US5635387A

公开(公告)日：1997-06-03

申请号：US415752

申请日：1995-04-03

申请人： Rui G. Fei ,  Shelly Heimfeld ,  Billy W. Minshall ,  Ronald J. Berenson

发明人： Rui G. Fei ,  Shelly Heimfeld ,  Billy W. Minshall ,  Ronald J. Berenson

IPC分类号： C12N5/0789 ,  G01N33/50 ,  C12N5/00 ,  A61K35/14 ,  C12N5/02

CPC分类号： C12N5/0647 ,  G01N33/5002 ,  C12N2500/02 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/23

摘要： Methods for increasing the number of human hematopoietic precursor cells in vitro are provided. The methods generally comprise (a) separating human hematopoietic precursor cells from mature hematopoietic cells present in a blood product; (b) inoculating the separated precursor cells into a culture vessel containing a culture medium comprising a nutritive medium and a source of growth factors at a density of between 1.times.10.sup.3 cells/ml and 4.times.10.sup.6 cells/ml; and (c) culturing the cells under conditions and for a time sufficient to increase the number of precursor cells relative to the number of such cells present in the blood product. The culture medium may also include a suitable amount of microcarrier beads. Suitable blood products include bone marrow, umbilical cord blood, and peripheral blood. A device for carrying out such methods is also provided.

摘要翻译： 提供了增加体外人造血前体细胞数量的方法。 所述方法通常包括（a）将血液产品中存在的成熟造血细胞的人类造血前体细胞分离出来; （b）将分离的前体细胞接种到含有密度为1×10 3个细胞/ ml和4×10 6个细胞/ ml之间的包含营养培养基和生长因子源的培养基的培养容器中; 和（c）在足以增加前体细胞数量的条件下培养细胞相对于存在于血液制品中的细胞数目。 培养基也可以包含适量的微载体珠。 合适的血液制品包括骨髓，脐带血和外周血。 还提供了一种用于执行这种方法的装置。

公开(公告)号：US5087570A

公开(公告)日：1992-02-11

申请号：US192378

申请日：1988-05-10

申请人： Irving L. Weissman ,  Gerald J. Spangrude ,  Christa Muller-Sieburg ,  Shelly Heimfeld

发明人： Irving L. Weissman ,  Gerald J. Spangrude ,  Christa Muller-Sieburg ,  Shelly Heimfeld

IPC分类号： C12N5/06 ,  A61K35/28 ,  C07K14/52 ,  G01N33/50

CPC分类号： A61K35/28 ,  C07K14/52 ,  G01N33/5005 ,  Y10S436/808

摘要： Highly concentrated hematopoietic stem cell compositions are provided which are substantially free of differentiated or dedicated hematopoietic cells. The cells are obtained by subtraction of cells having particular markers and selection of cells having particular markers. The resulting composition may be used to provide for individual or groups of hematopoietic lineages, to reconstitute stem cells of the host, and to identify an assay for a wide variety of hematopoietic growth factors.