公开(公告)号：US5507287A

公开(公告)日：1996-04-16

申请号：US428494

申请日：1995-04-27

申请人： Branko Palcic ,  Calum MacAulay ,  Bruno Jaggi ,  Stephen Lam ,  Amedeus E. Profio ,  Jaclyn Hung

发明人： Branko Palcic ,  Calum MacAulay ,  Bruno Jaggi ,  Stephen Lam ,  Amedeus E. Profio ,  Jaclyn Hung

IPC分类号： A61B1/00 ,  A61B1/04 ,  A61B5/00 ,  A61B1/06 ,  A61B1/267 ,  G01N21/64

CPC分类号： A61B1/043 ,  A61B1/00186 ,  A61B1/042 ,  A61B1/0638 ,  A61B1/0646 ,  A61B5/0071 ,  A61B5/0084 ,  A61B1/063 ,  G01N2021/6463

摘要： Apparatus for imaging diseases in tissue comprising a light source for generating excitation light that includes wavelengths capable of generating characteristic autofluorescence for abnormal and normal tissue. A fibreoptic illuminating light guide is used to illuminate tissue with light that includes at least the excitation light thereby exciting the tissue to emit the characteristic autofluorescence. An imaging bundle collects emitted autofluorescence light from the tissue. The autofluorescence light is filtered into spectral bands in which the autofluorescence intensity for abnormal tissue is substantially different from normal tissue and the autofluorescence intensity for abnormal tissue is substantially similar to normal tissue. An optical system is used to intercept the filtered autofluorescence light to acquire at least two filtered emitted autofluorescence images of the tissue. The acquired images are displayed in real time on a display monitor in such a manner as to delineate abnormal and normal tissue.

摘要翻译： 用于在组织中成像疾病的装置，包括用于产生激发光的光源，所述激发光包括能够产生用于异常和正常组织的特征性自发荧光的波长。 光纤照明光导用于用至少包括激发光的光照射组织，从而激发组织以发出特征自发荧光。 成像束从组织中收集发射的自发荧光。 自发荧光被过滤到光谱带，其中异常组织的自发荧光强度与正常组织基本不同，异常组织的自发荧光强度基本上与正常组织相似。 光学系统用于截取滤波的自发荧光以获得组织的至少两个经滤波的发射的自发荧光图像。 所获取的图像以描绘异常和正常组织的方式在显示监视器上实时显示。

公开(公告)号：US5590660A

公开(公告)日：1997-01-07

申请号：US218662

申请日：1994-03-28

申请人： Calum MacAulay ,  Branko Palcic ,  Stephen Lam ,  Bruno Jaggi

发明人： Calum MacAulay ,  Branko Palcic ,  Stephen Lam ,  Bruno Jaggi

IPC分类号： A61B10/00 ,  A61B1/00 ,  A61B1/05 ,  A61B5/00 ,  G01N21/64 ,  A61B1/06 ,  A61B6/00

CPC分类号： A61B1/043 ,  A61B1/05 ,  A61B5/0071 ,  A61B5/0084 ,  A61B5/0086 ,  H04N2005/2255

摘要： An apparatus and method for imaging diseases in tissue are presented. The apparatus employs a light source for producing excitation light to excite the tissue to generate autofluorescence light and for producing illumination light to generate reflected and back scattered light (remittance light) from the tissue. Optical sensors are used to receive the autofluorescence light and the remittance light to collect an autofluorescence light image and a remittance light image. A filter acts to integrate the autofluorescence image over a range of wavelengths in which the autofluorescence intensity for normal tissue is substantially different from the autofluorescence intensity for diseased tissue to establish an integrated autofluorescence image of the tissue. The remittance light image provides a background image to normalize the autofluorescence image to account for image non-uniformity due to changes in distance, angle and illumination intensity. A monitor displays the integrated autofluorescence image and the remittance light image to produce a normalized image in which diseased tissue is distinguishable from normal tissue. The optical sensor can be installed adjacent the end of an endoscope probe inserted into a body cavity. A method for imaging diseased tissue using an integrated fluorescence image and a normalizing remittance image is also disclosed.

摘要翻译： 提出了一种用于成像组织疾病成像的装置和方法。 该装置采用光源产生激发光以激发组织以产生自发荧光并产生照明光，以从组织产生反射和反向散射光（汇流光）。 光传感器用于接收自发荧光和汇流光以收集自发荧光图像和汇流光图像。 滤光器用于将自发荧光图像整合在一定波长范围内，其中正常组织的自发荧光强度基本上不同于患病组织的自发荧光强度，以建立组织的集成自发荧光图像。 汇款光图像提供背景图像以归一化自发荧光图像，以解决由于距离，角度和照明强度的变化引起的图像不均匀性。 监视器显示集成的自发荧光图像和汇流光图像以产生其中患病组织与正常组织区分开的标准化图像。 光学传感器可以安装在插入体腔的内窥镜探针的末端附近。 还公开了使用集成荧光图像和归一化汇款图像对患病组织进行成像的方法。

公开(公告)号：US5827190A

公开(公告)日：1998-10-27

申请号：US699607

申请日：1996-08-19

申请人： Branko Palcic ,  Calum MacAulay ,  Stephen Lam ,  Bruno Jaggi

发明人： Branko Palcic ,  Calum MacAulay ,  Stephen Lam ,  Bruno Jaggi

IPC分类号： A61B10/00 ,  A61B1/00 ,  A61B1/05 ,  A61B5/00 ,  G01N21/64 ,  A61B6/00

CPC分类号： A61B1/043 ,  A61B1/05 ,  A61B5/0071 ,  A61B5/0084 ,  A61B5/0086 ,  H04N2005/2255

摘要： An apparatus and method for imaging diseases in tissue are presented. The apparatus employs a light source for producing excitation light to excite the tissue to generate autofluorescence light and for producing illumination light to generate reflected and back scattered light (remittance light) from the tissue. Optical sensors are used to receive the autofluorescence light and the remittance light to collect an autofluorescence light image and a remittance light image. A filter acts to integrate the autofluorescence image over a range of wavelengths in which the autofluorescence intensity for normal tissue is substantially different from the autofluorescence intensity for diseased tissue to establish an integrated autofluorescence image of the tissue. The remittance light image provides a background image to normalize the autofluorescence image to account for image non-uniformity due to changes in distance, angle and illumination intensity. A monitor displays the integrated autofluorescence image and the remittance light image to produce a normalized image in which diseased tissue is distinguishable from normal tissue. The optical sensor can be installed adjacent the end of an endoscope probe inserted into a body cavity. A method for imaging diseased tissue using an integrated fluorescence image and a normalizing remittance image is also disclosed.

摘要翻译： 提出了一种用于成像组织疾病成像的装置和方法。 该装置采用光源产生激发光以激发组织以产生自发荧光并产生照明光，以从组织产生反射和反向散射光（汇流光）。 光传感器用于接收自发荧光和汇流光以收集自发荧光图像和汇流光图像。 滤光器用于将自发荧光图像整合在一定波长范围内，其中正常组织的自发荧光强度基本上不同于患病组织的自发荧光强度，以建立组织的集成自发荧光图像。 汇款光图像提供背景图像以归一化自发荧光图像，以解决由于距离，角度和照明强度的变化引起的图像不均匀性。 监视器显示集成的自发荧光图像和汇流光图像以产生其中患病组织与正常组织区分开的标准化图像。 光学传感器可以安装在插入体腔的内窥镜探针的末端附近。 还公开了使用集成荧光图像和归一化汇款图像对患病组织进行成像的方法。

公开(公告)号：US6069689A

公开(公告)日：2000-05-30

申请号：US59885

申请日：1998-04-14

申请人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

IPC分类号： A61B5/00 ,  A61B5/103 ,  G01N21/64 ,  G01N21/65 ,  G01B9/02 ,  G01N11/00

CPC分类号： G01N21/474 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/444 ,  G01N21/645 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/475 ,  G01N2021/6417 ,  G01N2021/656 ,  G01N2201/0627

摘要： Apparatus for diagnosis of a skin disease site using spectral analysis includes a light source for generating light to illuminate the disease site and a probe unit optically connected to the light source for exposing the disease site to light to generate fluorescence and reflectance light. The probe unit also collects the generated fluorescence and reflectance light and transmits this light to a spectrometer to be analyzed. The spectrometer generates and displays spectral measurements of the fluorescence light and the reflectance light which in together assist the user in diagnosing the disease site. The apparatus makes use of a conventional personal computer using a plug-in spectrometer card to provide a compact and low costs system. The system performs combined fluorescence and reflectance spectral analysis in a quick and efficient manner to provide a powerful tool for dermatologic diagnosis.

摘要翻译： 用于使用光谱分析诊断皮肤疾病部位的装置包括用于产生照亮疾病部位的光的光源和与光源光学连接的探针单元，用于将疾病部位暴露于光以产生荧光和反射光。 探针单元还收集产生的荧光和反射光，并将该光传输到要分析的光谱仪。 光谱仪产生和显示荧光和反射光的光谱测量，这些在一起协助用户诊断疾病部位。 该装置利用使用插入式光谱仪卡的常规个人计算机来提供紧凑且低成本的系统。 该系统以快速有效的方式进行组合荧光和反射光谱分析，为皮肤病诊断提供强大的工具。

公开(公告)号：US6128525A

公开(公告)日：2000-10-03

申请号：US901999

申请日：1997-07-29

申请人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

IPC分类号： A61B18/20 ,  A61N5/06 ,  A61N5/067 ,  A61B6/00

CPC分类号： A61N5/062 ,  A61B1/043 ,  A61B18/20 ,  A61B2018/00904 ,  A61N2005/0644 ,  A61N2005/067

摘要： A method and apparatus for controlling the dosimetry of a photodynamic therapy that involves exposing a site to be treated to treatment light in order to generate toxic products at the site and other photoproducts. Often a photosensitizer drug is administered to the patient prior to treatment or the therapy relies on the presence of endogenous photosensitizers. The method comprises the steps of selecting a photoproduct having an identifying characteristic, which can be a fluorescence peak, and monitoring the photoproduct using the identifying characteristic (e.g. fluorescence) to determine the level of the photoproduct being generated. The photodynamic therapy is then terminated when the photoproduct being monitored reaches a predetermined level. The method allows for safe treatment of a site using photodynamic therapy and ensures that overexposure to treatment light leading to damage of normal tissue or underexposure leading to ineffective treatment of the lesion does not occur. Apparatus for automatically carrying out the method is also provided.

摘要翻译： 一种用于控制光动力疗法的剂量测定的方法和装置，其涉及将待治疗的部位暴露于治疗光，以便在该部位产生有毒产物和其它光产物。 在治疗之前通常将光敏剂药物施用于患者，或者治疗依赖于内源性光敏剂的存在。 该方法包括以下步骤：选择具有识别特征的光产物，其可以是荧光峰，并使用识别特征（例如荧光）监测光产物，以确定所产生的光产物的水平。 当被监测的光产物达到预定水平时，光动力疗法终止。 该方法允许使用光动力疗法安全治疗部位，并且确保不会发生过度暴露于治疗光，导致正常组织损伤或曝光不足导致病变无效治疗。 还提供了用于自动执行该方法的装置。

公开(公告)号：US6008889A

公开(公告)日：1999-12-28

申请号：US843605

申请日：1997-04-16

申请人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

IPC分类号： A61B5/00 ,  A61B5/103 ,  G01N21/64 ,  G01N21/65 ,  G01B9/02 ,  G01N11/00

CPC分类号： G01N21/474 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/444 ,  G01N21/645 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/475 ,  G01N2021/6417 ,  G01N2021/656 ,  G01N2201/0627

摘要： Apparatus for diagnosis of a skin disease site using spectral analysis includes a light source for generating light to illuminate the disease site and a probe unit optically connected to the light source for exposing the disease site to light to generate fluorescence and reflectance light. The probe unit also collects the generated fluorescence and reflectance light and transmits this light to a spectrometer to be analysed. The spectrometer generates and displays spectral measurements of the fluorescence light and the reflectance light which in together assist the user in diagnosing the disease site. The apparatus makes use of a conventional personal computer using a plug-in spectrometer card to provide a compact and low cost system. The system performs combined fluorescence and reflectance spectral analysis in a quick and efficient manner to provide a powerful tool for dermatologic diagnosis.

摘要翻译： 用于使用光谱分析诊断皮肤疾病部位的装置包括用于产生照亮疾病部位的光的光源和与光源光学连接的探针单元，用于将疾病部位暴露于光以产生荧光和反射光。 探针单元还收集产生的荧光和反射光，并将该光传输到要分析的光谱仪。 光谱仪产生和显示荧光和反射光的光谱测量，这些在一起协助用户诊断疾病部位。 该装置利用使用插入式光谱仪卡的常规个人计算机来提供紧凑且低成本的系统。 该系统以快速有效的方式进行组合荧光和反射光谱分析，为皮肤病诊断提供强大的工具。

公开(公告)号：US20070188856A1

公开(公告)日：2007-08-16

申请号：US11499086

申请日：2006-08-04

申请人： Calum MacAulay

发明人： Calum MacAulay

IPC分类号： G02B21/06

CPC分类号： G02B21/36 ,  G02B21/006 ,  G02B21/0072 ,  G02B21/06 ,  G02B26/0841 ,  Y10S359/90

摘要： Apparatus and methods relating to microscopes having specific control of the light that contacts a sample and/or a light detector, such as the eye of the user, a charge couple device or a video camera. The improved control includes enhanced, selective control of the angle of illumination, quantity of light and location of light reaching the sample and/or detector. The microscopes comprise one or more spatial light modulators in the illumination and/or detection light path of the microscope at one or both of the conjugate image plane of the aperture diaphragm of the objective lens and the conjugate image plane of the sample.

摘要翻译： 与具有与样品和/或光检测器（例如用户的眼睛，电荷耦合器件或摄像机）接触的光的具体控制的显微镜相关的装置和方法。 改进的控制包括对照射角度，光量和到达样品和/或检测器的光的位置的增强的选择性控制。 显微镜在物镜的孔径光阑的共轭像平面和样品的共轭像平面的一个或两个处，在显微镜的照明和/或检测光路中包括一个或多个空间光调制器。

公开(公告)号：US20070240527A1

公开(公告)日：2007-10-18

申请号：US11285839

申请日：2005-11-23

申请人： Calum MacAulay ,  Jagoda Korbelik ,  Mark Cardeno

发明人： Calum MacAulay ,  Jagoda Korbelik ,  Mark Cardeno

IPC分类号： G01N1/00 ,  G01N1/02

CPC分类号： B01L3/0265 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00367 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00531 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01L3/021 ,  B01L3/0262 ,  B01L3/0275 ,  B01L2400/0633 ,  B82Y30/00 ,  C40B60/14 ,  G01N33/80 ,  Y10T436/2575

摘要： Microvolume liquid dispensers that provide simple and inexpensive approaches to making cytology microarrays. In some embodiments, the dispensers comprise tips that comprise an outer sleeve, typically shaped like a funnel, that holds a reciprocating needle or pin. The tip of the pin slightly extends beyond the distal opening of the outer sleeve in one position, and is retracted in another position. When the pin is in the distal position the pin contacts the inner surfaces of the sleeve and blocks cytology liquid from flowing through the opening of the sleeve. Thus the pin and sleeve cooperate to form a reservoir behind the blockage. When the pin is pushed up into the sleeve, for example by touching the tip to a glass slide, a passage is formed between the outer surface of the pin and the inner surface of the sleeve. The liquid in the reservoir then flows through the passage and onto the slide. Removing the tip from the substrate moves the pin back to its original position, re-forming the reservoir and leaving a predetermined microvolume amount of the liquid on the slide.

摘要翻译： 微量液体分配器提供简单和便宜的方法来制备细胞学微阵列。 在一些实施例中，分配器包括尖端，其包括保持往复式针或销的通常形状为漏斗的外套筒。 销的尖端在一个位置稍微延伸超过外套筒的远端开口，并且缩回到另一位置。 当销处于远端位置时，销接触套筒的内表面并阻止细胞液体流过套筒的开口。 因此，销和套筒协作以在堵塞物后面形成储存器。 当销被推入套筒时，例如通过将尖端触及玻璃滑块，在销的外表面和套筒的内表面之间形成通道。 然后，储存器中的液体流过通道并流到载玻片上。 从基板移除尖端将销移回到其初始位置，重新形成储存器并在载玻片上留下预定的微量的液体。

公开(公告)号：US20050269523A1

公开(公告)日：2005-12-08

申请号：US11185069

申请日：2005-07-19

申请人： Calum MacAulay ,  Pierre Lane

发明人： Calum MacAulay ,  Pierre Lane

IPC分类号： G01N21/27 ,  G01N21/64

CPC分类号： G01N21/274 ,  G01N21/6452 ,  G01N2201/12792

摘要： Microarray readers and methods that compensate for target spots that are too dim or too bright for the microarray reader to accurately measure. The readers adjust the amount of light directed at or received from specific non-acceptable target spots, such that dim spots receive more excitation light and overly bright spots receive less. This increases or decreases, respectively, their measured brightness, which in turn effectively increases the range over which a microarray reader can accurately measure the spots, and can also improve the signal-to-noise ratio and other aspects of the measurements.

摘要翻译： 微阵列阅读器和补偿微阵列阅读器准确测量的目标斑点太暗或太亮的方法。 读者调节指向或从特定不可接受的目标点接收的光量，使得暗点接收更多的激发光并且过亮点接收较少。 它们分别增加或减少它们的测量亮度，这反过来有效地增加了微阵列读取器可精确测量斑点的范围，并且还可以提高测量的信噪比和其他方面。