公开(公告)号：US20190336969A1

公开(公告)日：2019-11-07

申请号：US16367036

申请日：2019-03-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  University of Chicago

Inventor： Rustem F. ISMAGILOV ,  Liang MA ,  Qichao PAN ,  Mikhail KARYMOV ,  Toan HUYNH ,  George SAWICKI ,  Stefano BEGOLO ,  Wenbin DU

IPC: B01L3/00 ,  C12Q1/686 ,  C12M3/00 ,  C12Q1/02 ,  C12M1/12 ,  B01F13/00

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

公开(公告)号：US20180321137A1

公开(公告)日：2018-11-08

申请号：US15774004

申请日：2016-11-04

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jesus Rodriguez MANZANO ,  Mikhail KARYMOV ,  David A. SELCK ,  Stefano BEGOLO ,  Erik B. JUE ,  Dmitriy V. ZHUKOV

IPC: G01N21/25 ,  G06K9/00 ,  G06T7/90 ,  G01J3/50 ,  G01J3/02 ,  C12Q1/6844

CPC classification number: G01N21/253 ,  C12Q1/6844 ,  G01J3/0272 ,  G01J3/46 ,  G01J3/50 ,  G01N2201/0221 ,  G06K9/00134 ,  G06T7/00 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/30024 ,  C12Q2527/101 ,  C12Q2537/165 ,  C12Q2563/159

Abstract: The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.

公开(公告)号：US20160114322A1

公开(公告)日：2016-04-28

申请号：US14785480

申请日：2014-04-18

Applicant: Qichao PAN ,  Mikhail KARYMOV ,  Toan HUYNH ,  George SAWICKI ,  Stefano BEGOLO ,  Wenbin DU ,  CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF CHICAGO

Inventor： Rustem F. ISMAGILOV ,  Liang MA ,  Qichao PAN ,  Mikhail KARYMOV ,  Toan HUYNH ,  George SAWICKI ,  Stefano BEGOLO ,  Wenbin DU

IPC: B01L3/00 ,  C12Q1/68 ,  C12Q1/02

CPC classification number: B01L3/502715 ,  B01F13/0071 ,  B01F13/0083 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/045 ,  B01L2300/047 ,  B01L2300/048 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/065 ,  C12M23/44 ,  C12M23/46 ,  C12M25/06 ,  C12Q1/02 ,  C12Q1/025 ,  C12Q1/686 ,  G01N2035/00158

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

Abstract translation: 本文提供了用于平行处理样品（例如细胞或其他生物样品）的方法，组合物和装置。 方法，组合物和装置适合于多种水平的分析，包括样品的遗传和功能测定。