公开(公告)号：US20170225161A1

公开(公告)日：2017-08-10

申请号：US15504283

申请日：2015-08-14

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Stefano BEGOLO ,  Dmitriy V. ZHUKOV ,  Daan WITTERS ,  Erik B. JUE ,  Rustem F. ISMAGILOV

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2300/041 ,  B01L2300/046 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/14 ,  B01L2400/0475 ,  B01L2400/0478 ,  B01L2400/0487 ,  C12Q1/686

Abstract: Provided herein are devices and methods for generating positive and negative pressures. The devices and methods are suited for the generation of pressures; in particular, the pressures generated can be useful for controlling the flow of fluids, such as in fluidic device.

公开(公告)号：US20190118177A1

公开(公告)日：2019-04-25

申请号：US16141707

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Eugenia KHOROSHEVA ,  Dmitriy V. ZHUKOV

IPC: B01L3/00 ,  C12Q1/6855

Abstract: A device for allowing compartmentalized reactions with minimized cross-contamination between the compartments, utilizing a delivery of material by loading wells to pooling wells, such that the pooling wells can be additively provided with reactants while maintaining isolation between the pooling wells. The use of geometric properties is used to facilitate transmission of fluids/droplets without the need for hydrophilic surfaces.

公开(公告)号：US20180321137A1

公开(公告)日：2018-11-08

申请号：US15774004

申请日：2016-11-04

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. ISMAGILOV ,  Jesus Rodriguez MANZANO ,  Mikhail KARYMOV ,  David A. SELCK ,  Stefano BEGOLO ,  Erik B. JUE ,  Dmitriy V. ZHUKOV

IPC: G01N21/25 ,  G06K9/00 ,  G06T7/90 ,  G01J3/50 ,  G01J3/02 ,  C12Q1/6844

CPC classification number: G01N21/253 ,  C12Q1/6844 ,  G01J3/0272 ,  G01J3/46 ,  G01J3/50 ,  G01N2201/0221 ,  G06K9/00134 ,  G06T7/00 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/30024 ,  C12Q2527/101 ,  C12Q2537/165 ,  C12Q2563/159

Abstract: The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.