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1.发明公开公开(公告)号：US20240084262A1
公开(公告)日：2024-03-14
申请号：US18268822
申请日：2021-10-29
发明人： Dhruv Sareen , Stephen Pandol , Syairah Hanan Shaharuddin , Victoria Wang , Michael Edison P. Ramos , Aurelia Lugea , Richard T. Waldron
CPC分类号： C12N5/0677 , C12M23/16 , C12M23/34 , C12M25/02 , C12M25/14 , C12N5/0031 , G01N33/507 , C12N2500/32 , C12N2500/38 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/155 , C12N2501/16 , C12N2501/385 , C12N2501/41 , C12N2501/415 , C12N2506/45
摘要： The present invention provides for methods of differentiating induced pluripotent stem cells into pancreatic progenitor cells, pancreatic ductal cells, pancreatic endocrine cells, pancreatic acinar cells, and pancreatic organoids. Cells created by these methods are also provided. Further provided are disease models and methods of drug screening.
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公开(公告)号：US11326149B2
公开(公告)日：2022-05-10
申请号：US16051004
申请日：2018-07-31
发明人： S. Jordan Kerns , Norman Wen , Carol Lucchesi , Christopher David Hinojosa , Jacob Fraser , Jefferson Puerta , Geraldine Hamilton , Robert Barrett , Clive Svendsen , Daniel Levner , Stephen R Targan , Michael Workman , Dhruv Sareen , Uthra Rajamani , Magdalena Kasendra
摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
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公开(公告)号：US20190031992A1
公开(公告)日：2019-01-31
申请号：US16051004
申请日：2018-07-31
发明人： S. Jordan Kerns , Norman Wen , Carol Lucchesi , Christopher David Hinojosa , Jacob Fraser , Jefferson Puerta , Geraldine Hamilton , Robert Barrett , Clive Svendsen , Daniel Levner , Stephen R. Targan , Michael Workman , Dhruv Sareen , Uthra Rajamani , Magdalena Kasendra
摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
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公开(公告)号：US20180057788A1
公开(公告)日：2018-03-01
申请号：US15352289
申请日：2016-11-15
发明人： Jordan Kerns , Norman Wen , Carol Lucchesi , Christopher Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Sam Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
IPC分类号： C12N5/00 , C12N5/0797 , B01L3/00
CPC分类号： C12N5/0068 , B01L3/502715 , B01L2300/044 , B01L2300/0858 , B01L2300/123 , C12M23/16 , C12M25/02 , C12N5/0623 , C12N5/069 , C12N2502/088 , C12N2531/00 , C12N2535/10 , C12N2539/00
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US11970714B2
公开(公告)日：2024-04-30
申请号：US16919456
申请日：2020-07-02
发明人： Dhruv Sareen , Loren A. Ornelas , Clive Svendsen
IPC分类号： C12N5/074
CPC分类号： C12N5/0696 , C12N2500/25 , C12N2501/115 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/608 , C12N2502/02 , C12N2506/11 , C12N2533/52
摘要： Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.
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公开(公告)号：US11473061B2
公开(公告)日：2022-10-18
申请号：US16074747
申请日：2017-02-01
发明人： Robert Barrett , Clive Svendsen , Stephan R. Targan , Michael Workman , Dhruv Sareen , Uthra Rajamani
摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
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公开(公告)号：US11174462B2
公开(公告)日：2021-11-16
申请号：US15955335
申请日：2018-04-17
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sanees , Clive Svendsen , Daniel Levner , Dhruv Sareen
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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8.发明申请公开(公告)号：US20190136203A1
公开(公告)日：2019-05-09
申请号：US16247341
申请日：2019-01-14
发明人： Dhruv Sareen , Loren A. Ornelas , Clive Svendsen
IPC分类号： C12N5/074
摘要： Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.
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公开(公告)号：US20180305651A1
公开(公告)日：2018-10-25
申请号：US15768736
申请日：2016-10-19
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
IPC分类号： C12M3/06 , C12N5/0793 , C12N5/071 , B01L3/00
CPC分类号： C12N5/0619 , B01L3/5027 , B01L2200/0647 , B01L2300/0861 , C12M23/16 , C12M25/02 , C12M35/08 , C12N5/0618 , C12N5/0622 , C12N5/069 , C12N5/0692 , C12N2502/081 , C12N2502/086 , C12N2502/28 , C12N2506/45 , C12N2531/00 , C12N2533/52 , C12N2533/54 , C12N2533/56 , C12N2535/10 , C12N2539/00 , C12Q1/02
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US20180298332A1
公开(公告)日：2018-10-18
申请号：US15955383
申请日：2018-04-17
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
IPC分类号： C12N5/0793 , C12N5/071 , C12M3/06 , C12M1/12 , C12M1/42
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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