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公开(公告)号：US20110217238A1
公开(公告)日：2011-09-08
申请号：US13061530
申请日：2009-09-01
IPC分类号： A61K49/00 , C12Q1/68 , C12Q1/02 , G01N33/574
CPC分类号： G01N33/57449 , C12Q1/6886 , C12Q2600/118 , C12Q2600/158 , G01N2500/00 , G01N2800/56
摘要： The present invention provides a binding moiety which selectively binds to Sox11 protein and/or mRNA for imaging, diagnosis or prognosis of epithelial ovarian cancer (EOC). Optionally, the moiety is an antibody or antigen-binding fragment thereof. Advantageously, moiety comprises a further, readily detectable moiety. The invention also provides methods of imaging EOC cells as well as methods of diagnosing or prognosing EOC in an individual. A further aspect of the present invention provides a method of identifying cells associated with EOC, the method comprising analysing the pattern of gene expression in a sample of cells to be tested and comparing it to the pattern of gene expression in a sample of known lymphomas cells. Preferably, the cells to be tested are identified as EOC cells if the expression of Sox11 is up-regulated compared to normal B-cells. Preferably EOC cells are identified as improved recurrence-free survival-associated if expression of Sox11 is up-regulated compared with non-cancerous epithelial ovarian cells. Preferably, EOC cells are identified as diminished recurrence-free survival-associated if expression of Sox11 is similar to, or down-regulated, compared with non-cancerous epithelial ovarian cells.
摘要翻译： 本发明提供了选择性结合Sox11蛋白和/或mRNA用于上皮性卵巢癌（EOC）成像，诊断或预后的结合部分。 任选地，该部分是抗体或其抗原结合片段。 有利地，部分包含另外易于检测的部分。 本发明还提供对EOC细胞进行成像的方法以及在个体中诊断或预测EOC的方法。 本发明的另一方面提供了鉴定与EOC相关的细胞的方法，所述方法包括分析待测试细胞样品中基因表达的模式，并将其与已知淋巴瘤细胞样品中的基因表达模式进行比较 。 如果与正常B细胞相比，如果Sox11的表达被上调，则待测试的细胞被鉴定为EOC细胞。 如果与非癌性上皮性卵巢细胞相比，如果Sox11的表达上调，则优选EOC细胞被鉴定为改善的无复发存活相关性。 优选地，与非癌性上皮性卵巢细胞相比，如果Sox11的表达与非癌性上皮性卵巢细胞相似或下调，则EOC细胞被鉴定为减少的无复发存活相关。
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公开(公告)号：US20120178698A1
公开(公告)日：2012-07-12
申请号：US13378805
申请日：2010-06-18
CPC分类号： A61K31/7088
摘要： The present invention provides agents capable of activating Sox11 for use in medicine. In particular, the agents of the invention are useful in the treatment of cancers, such as lymphomas (e.g. mantel cell lymphoma). The invention further provides pharmaceutical compositions of the agents of the invention, as well as methods and uses of the same.
摘要翻译： 本发明提供能够激活用于药物的Sox11的药剂。 特别地，本发明的药剂可用于治疗癌症，例如淋巴瘤（例如壁底细胞淋巴瘤）。 本发明还提供了本发明的药剂的药物组合物，以及其用途和用途。
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3.发明授权公开(公告)号：US09486520B2
公开(公告)日：2016-11-08
申请号：US13639406
申请日：2011-04-13
申请人： Carl Arne Krister Borrebaeck , Sissela Rachel Linn Broos , Peter Bo Joakim Ellmark , Malin Marie Lindstedt
发明人： Carl Arne Krister Borrebaeck , Sissela Rachel Linn Broos , Peter Bo Joakim Ellmark , Malin Marie Lindstedt
CPC分类号： A61K39/39 , A61K9/0019 , A61K9/146 , A61K9/5146 , A61K2039/55516 , A61K2039/55555
摘要： The present invention provides pharmaceutical compositions comprising an immunostimulatory polypeptide and polyglutamic acid (PGA) nanoparticles, formulated in a pharmaceutically acceptable diluent, carrier or excipient. Such compositions have utility in stimulating the immune system in subjects, with the components capable of interacting synergistically. The invention further provides uses of the compositions of the invention, for example in the treatment of cancer, and kit and components for use in the same.
摘要翻译： 本发明提供包含免疫刺激多肽和聚谷氨酸（PGA）纳米颗粒的药物组合物，其配制在药学上可接受的稀释剂，载体或赋形剂中。 这些组合物可有效地刺激受试者的免疫系统，其中组分能够协同相互作用。 本发明进一步提供本发明组合物的用途，例如用于治疗癌症，以及用于其中的试剂盒和组分。
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公开(公告)号：US20110287486A1
公开(公告)日：2011-11-24
申请号：US13110412
申请日：2011-05-18
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
摘要： The present invention relates to a method for in vitro creation of molecular libraries evolution of protein function. Particularly, it relates to variability and modification of protein function by shuffling polynucleotide sequence segments. A protein of desired characteristics can be obtained by incorporating variant peptide regions (variant motifs) into defined peptide regions (scaffold sequence). The variant motifs can be obtained from parent DNA which has been subjected to mutagenesis to create a plurality of differently mutated derivatives thereof or they can be obtained from in vivo sequences. These variant motifs can then be incorporated into a scaffold sequence and the resulting coded protein screened for desired characteristics. This method is ideally used for obtaining antibodies with desired characteristics by isolating individual CDR DNA sequences and incorporating them into a scaffold which may, for example, be from a totally different antibody.
摘要翻译： 本发明涉及用于体外产生分子文库蛋白质功能进化的方法。 特别地，其涉及通过混洗多核苷酸序列片段的蛋白质功能的变异性和修饰。 通过将变体肽区域（变体基序）并入限定的肽区域（支架序列）中可获得所需特征的蛋白质。 变体基序可以从已经进行诱变以产生多个不同突变的衍生物的亲本DNA获得，或者可以从体内序列获得。 然后可以将这些变体基序并入支架序列中，并筛选所得到的编码蛋白质以获得所需特征。 该方法理想地用于通过分离各个CDR DNA序列并将其并入到例如来自完全不同的抗体的支架中来获得具有期望特征的抗体。
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5.发明授权Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence 有权蛋白质功能的体外分子进化的方法涉及使用外切核酸酶和两个母体多核苷酸序列的群体公开(公告)号：US07153655B2
公开(公告)日：2006-12-26
申请号：US10321195
申请日：2002-12-17
申请人： Carl Arne Krister Borrebaeck , Ann-Christin Malmborg-Hager , Christina Furebring , Ulf Hans Eskil Soderlind , Rebecka Ingrid Camilla Ottosson
发明人： Carl Arne Krister Borrebaeck , Ann-Christin Malmborg-Hager , Christina Furebring , Ulf Hans Eskil Soderlind , Rebecka Ingrid Camilla Ottosson
CPC分类号： C12N15/1027
摘要： The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new proteins (e.g., antibodies and enzymes) or parts thereof having modified characteristics as compared to the parent protein.
摘要翻译： 本发明涉及蛋白质功能体外进化的方法。 特别地，该方法涉及从外切核酸酶消化获得的核苷酸片段的改组。 本发明人已经显示衍生自用外切核酸酶消化的亲代多核苷酸序列的多核苷酸片段可以组合以产生编码具有所需特征的多肽的多核苷酸序列。 与母体蛋白质相比，该方法可有效地应用于具有修饰特征的新蛋白质（例如抗体和酶）或其部分的产生。
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公开(公告)号：US20130143760A1
公开(公告)日：2013-06-06
申请号：US13702756
申请日：2011-06-09
IPC分类号： G01N33/68
CPC分类号： G01N33/6893 , G01N33/54366 , G01N33/57415
摘要： The present invention provides a method for the prognosis of breast cancer in a subject comprising the steps of: (a) providing a first proteome sample from the subject; (b) measuring in the first proteome sample the amount of one or more biomarkers selected from the group of biomarkers listed in Table 1; (c) providing an additional (e.g. a second) proteome sample from the subject; (d) measuring in the additional proteome sample the amount of the one or more biomarkers selected from the group of biomarkers listed in Table 1 measured in step (b); and (e) determining the difference between the amount of the one or more biomarkers in the first and additional proteome samples; wherein the first proteome sample and additional proteome sample are representative of the proteome composition of the subject on different days, and wherein the difference between the amount of the one or more biomarkers in the first and additional (e.g. second) proteome samples is indicative of the risk of recurrence and/or metastasis of the breast cancer in the subject. In further embodiments, step (b) comprises measuring the amount of each the biomarkers listed in Table 1(A) or all of the biomarkers listed in Table 1. The present invention additionally provides an array for performing the method of the invention, the use in vitro of a biomarker selected from the group of biomarkers in Table 1 as a prognostic marker for determining risk of recurrence and/or metastasis of breast cancer in a subject, and a kit for performing the method of the invention comprising one or more binding agents capable of binding to a biomarker listed in Table 1.
摘要翻译： 本发明提供了一种用于对象中乳腺癌预后的方法，包括以下步骤：（a）从受试者提供第一蛋白质组样品; （b）在第一蛋白质组样品中测量选自表1所列生物标志物组的一种或多种生物标志物的量; （c）从受试者提供另外的（例如第二）蛋白质组样品; （d）在另外的蛋白质组样品中测量选自在步骤（b）中测量的表1中列出的生物标志物组的一种或多种生物标志物的量; 和（e）确定第一和另外的蛋白质组样品中一种或多种生物标志物的量之间的差异; 其中所述第一蛋白质组样品和另外的蛋白质组样品在不同天数代表所述受试者的蛋白质组成组成，并且其中所述第一和另外（例如第二）蛋白质组样品中所述一种或多种生物标志物的量之间的差异指示 受试者乳腺癌复发和/或转移的风险。 在另外的实施方案中，步骤（b）包括测量表1（A）中列出的每种生物标志物的量或表1中列出的所有生物标志物的量。本发明另外提供了用于实施本发明方法的阵列， 选自表1中的生物标志物组的生物标志物的体外作为用于确定受试者的乳腺癌复发和/或转移风险的预后标记物，以及用于进行本发明方法的试剂盒，其包含一种或多种结合剂 能够结合表1中列出的生物标志物。
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公开(公告)号：US20100119449A1
公开(公告)日：2010-05-13
申请号：US12598221
申请日：2008-05-12
CPC分类号： C12Q1/6886 , A61K51/1069 , C07K16/00 , C07K16/18 , C12Q2600/118 , C12Q2600/158 , G01N33/57496 , G01N2500/20 , G01N2800/52
摘要： The present invention provides a binding moiety which selectively binds to Sox11 protein and/or mRNA for imaging, diagnosis or prognosis of lymphomas, such as mantle cell lymphomas (MCL) and diffuse large B-cell lymphoma (DLBCL). Optionally, the moiety is an antibody or antigen-binding fragment thereof. Advantageously, moiety comprises a further, readily detectable moiety. The invention also provides methods of imaging lymphomas cells as well as methods of diagnosing or prognosing lymphomas in an individual. A further aspect of the present invention provides a method of identifying cells associated with lymphomas, the method comprising analysing the pattern of gene expression in a sample of cells to be tested and comparing it to the pattern of gene expression in a sample of known lymphomas cells. Preferably, the cells to be tested are identified as lymphoma cells if the expression of Sox11 is upregulated compared to normal B-cells.
摘要翻译： 本发明提供了结合部分，其选择性地结合Sox11蛋白和/或mRNA用于淋巴瘤的成像，诊断或预后，例如套细胞淋巴瘤（MCL）和弥漫性大B细胞淋巴瘤（DLBCL）。 任选地，该部分是抗体或其抗原结合片段。 有利地，部分包含另外易于检测的部分。 本发明还提供了对淋巴瘤细胞进行成像的方法以及诊断或预测个体中淋巴瘤的方法。 本发明的另一方面提供了鉴定与淋巴瘤相关的细胞的方法，所述方法包括分析待测试细胞样品中基因表达的模式，并将其与已知淋巴瘤细胞样品中的基因表达模式进行比较 。 如果与正常B细胞相比，如果Sox11的表达上调，则优选将待测试的细胞识别为淋巴瘤细胞。
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公开(公告)号：US20100105571A1
公开(公告)日：2010-04-29
申请号：US12593448
申请日：2008-03-25
CPC分类号： G01N33/57438
摘要： The present invention provides a method for determining the presence of pancreatic adenocarcinoma in an individual and/or for determining the survival time of an individual afflicted with pancreatic adenocarcinoma comprising the steps of: (a) providing a serum or plasma sample to be tested; and (b) determining a protein signature of the test sample by measuring the presence and/or amount in the test sample of one or more selected proteins; wherein the presence and/or amount in the test sample of one or more proteins selected from the group defined in Table 1 is indicative of the presence of pancreatic adenocarcinoma. The invention also provides an array and a kit suitable for use in the methods of the invention.
摘要翻译： 本发明提供了确定个体中胰腺癌的存在和/或用于确定患有胰腺腺癌的个体的存活时间的方法，包括以下步骤：（a）提供待测试的血清或血浆样品; 和（b）通过测量一种或多种所选蛋白质的测试样品中的存在和/或量来确定测试样品的蛋白质特征; 其中选自表1中所定义的一组或多种蛋白质的测试样品中的存在和/或量指示存在胰腺腺癌。 本发明还提供适用于本发明方法的阵列和试剂盒。
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公开(公告)号：US06495321B1
公开(公告)日：2002-12-17
申请号：US09445649
申请日：2000-04-06
IPC分类号： C12Q168
CPC分类号： C40B40/02 , C12N15/1027 , C12N15/1037 , C12Q1/6811 , C12Q1/6858 , C12Q2521/319
摘要： The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleofide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.
摘要翻译： 本发明涉及蛋白质功能体外进化的方法。 特别地，该方法涉及从外切核酸酶消化获得的核苷酸片段的改组。 本发明人已经显示衍生自用核酸外切酶消化的亲本多核苷酸序列的多核苷酸片段可以组合以产生编码具有所需特征的多肽的多核苷酸序列。 与母体抗体相比，该方法可有效地应用于具有修饰特征的新抗体或其部分的产生。
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10.发明授权Method for in vitro molecular evolution of protein function using exonuclease and amplification 失效使用外切核酸酶和扩增的体外分子进化蛋白质功能的方法
公开(公告)号：US6159690A
公开(公告)日：2000-12-12
申请号：US98287
申请日：1998-06-16
IPC分类号： C12N15/09 , A61K38/00 , A61K38/43 , A61K39/395 , C12N15/10 , C12P21/02 , C12Q1/68 , C40B40/02 , C12P19/34
CPC分类号： C40B40/02 , C12N15/1027 , C12N15/1037 , C12Q1/6811 , C12Q1/6858 , C12Q2521/319
摘要： The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.
摘要翻译： 本发明涉及蛋白质功能体外进化的方法。 特别地，该方法涉及从外切核酸酶消化获得的核苷酸片段的改组。 本发明人已经显示衍生自用外切核酸酶消化的亲代多核苷酸序列的多核苷酸片段可以组合以产生编码具有所需特征的多肽的多核苷酸序列。 与母体抗体相比，该方法可有效地应用于具有修饰特征的新抗体或其部分的产生。
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