公开(公告)号：US20060013832A1

公开(公告)日：2006-01-19

申请号：US11029188

申请日：2005-01-04

申请人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

发明人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

IPC分类号： A61K39/21 ,  C12N15/86

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K38/00 ,  A61K39/02 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2039/5158 ,  C07K14/705 ,  C07K14/70521 ,  C07K14/70532 ,  C07K16/00 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2833 ,  C07K16/2866 ,  C07K16/289 ,  C07K16/2896 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/73 ,  C07K2317/74 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599 ,  C12N2710/00088 ,  C12N2740/16011

摘要： Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

摘要翻译： 描述了通过激活T细胞群并用结合附属分子的配体刺激T细胞表面上的辅助分子来诱导T细胞群体增殖的方法。 T细胞增殖发生在没有外源生长因子或附属细胞的情况下。 通过刺激T细胞受体（TCR）/ CD3复合物或CD2表面蛋白来完成T细胞活化。 为了诱导活化的群体T细胞的增殖，T细胞表面上的辅助分子如CD28被与配合分子结合的配体刺激。 通过本发明的方法扩增的T细胞群体可以被遗传转导并用于免疫治疗，或可用于诊断方法。

公开(公告)号：US20060140919A1

公开(公告)日：2006-06-29

申请号：US11366331

申请日：2006-03-02

申请人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

发明人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

IPC分类号： A61K39/395 ,  A61K35/14

CPC分类号： C07K14/705 ,  A61K2039/5158 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/2896 ,  C07K2317/34 ,  C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599

摘要： Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

公开(公告)号：US20060099177A1

公开(公告)日：2006-05-11

申请号：US11326148

申请日：2006-01-05

申请人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

发明人： Carl June ,  Craig Thompson ,  Gary Nabel ,  Gary Gray ,  Paul Rennert

IPC分类号： A61K35/14 ,  C12N5/08

CPC分类号： C07K16/2809 ,  A61K35/17 ,  A61K2039/507 ,  C07K16/2806 ,  C07K16/2818 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/74 ,  C07K2319/30 ,  C12N2740/16011

摘要： Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

公开(公告)号：US20060205069A1

公开(公告)日：2006-09-14

申请号：US11364102

申请日：2006-02-28

申请人： Carl June ,  Craig Thompson ,  Gary Gray ,  Paul Rennert

发明人： Carl June ,  Craig Thompson ,  Gary Gray ,  Paul Rennert

IPC分类号： G01N33/551 ,  C12N5/06

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/505 ,  A61K2039/5158 ,  C07K14/705 ,  C07K14/70521 ,  C07K14/70532 ,  C07K16/00 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2833 ,  C07K16/2866 ,  C07K16/289 ,  C07K16/2896 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/73 ,  C07K2317/74 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599 ,  C12N2740/16011 ,  C12N2795/00011

摘要： Compositions for inducing a population of T cells to proliferate in vitro by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population of T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the composition of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis

摘要翻译： 描述了通过激活T细胞群并用结合辅助分子的配体刺激T细胞表面上的辅助分子来诱导T细胞群体在体外增殖的组合物。 T细胞增殖发生在没有外源生长因子或附属细胞的情况下。 通过刺激T细胞受体（TCR）/ CD3复合物或CD2表面蛋白来完成T细胞活化。 为了诱导活化的T细胞群体的增殖，T细胞表面上的辅助分子例如CD28用与配体分子结合的配体刺激。 通过本发明的组合物扩增的T细胞群体可以被遗传转导并用于免疫治疗或可用于诊断方法

公开(公告)号：US20060246587A1

公开(公告)日：2006-11-02

申请号：US11476288

申请日：2006-06-28

申请人： Carl June ,  Craig Thompson ,  Suil Kim

发明人： Carl June ,  Craig Thompson ,  Suil Kim

IPC分类号： C12N15/867 ,  C12N5/08

CPC分类号： C07K16/2818 ,  A61K48/00 ,  C07K16/2809 ,  C12N15/87

摘要： A method for transfecting T cells with a nucleic acid molecule comprising a gene such that the gene is expressed in the T cells is described. The T cells are stimulated and proliferating prior to introduction of the nucleic acid molecule.

摘要翻译： 描述了用包含基因的核酸分子转染T细胞的方法，使得该基因在T细胞中表达。 T细胞在引入核酸分子之前被刺激并增殖。

公开(公告)号：US20060199780A1

公开(公告)日：2006-09-07

申请号：US11296725

申请日：2005-12-02

申请人： Carl June ,  Craig Thompson

发明人： Carl June ,  Craig Thompson

IPC分类号： A61K48/00 ,  A61K39/395

CPC分类号： C07K16/18 ,  C07K2317/73

摘要： Methods for protecting a T cell from cell death are described. The methods involve contacting the T cell with an agent which augments the bcl-XL protein level in the T cell such that it is protected from cell death. The invention further pertains to methods for increasing the susceptibility of a T cell to cell death, comprising contacting the T cell with at least one agent which decreases bcl-XL protein level in the T cell. Both in vivo and in vitro methods are described.

摘要翻译： 描述了保护T细胞免于细胞死亡的方法。 所述方法包括使T细胞与增加T细胞中的bcl-X L L蛋白水平的试剂接触，使得其被保护免于细胞死亡。 本发明还涉及增加T细胞对细胞死亡的易感性的方法，包括使T细胞与降低T细胞中bcl-XL蛋白水平的至少一种试剂接触。 描述体内和体外方法。

公开(公告)号：US08075921B2

公开(公告)日：2011-12-13

申请号：US12750374

申请日：2010-03-30

申请人： Daniel H. Fowler ,  Unsu Jung ,  Ronald E. Gress ,  Bruce Levine ,  Carl June

发明人： Daniel H. Fowler ,  Unsu Jung ,  Ronald E. Gress ,  Bruce Levine ,  Carl June

IPC分类号： A61K35/28

CPC分类号： A61K35/17 ,  A61K31/436 ,  A61K2035/122 ,  A61K2035/124 ,  A61K2039/57 ,  C12N5/0636 ,  C12N2501/04 ,  C12N2501/23 ,  C12N2501/51 ,  C12N2501/515 ,  A61K2300/00

摘要： Methods for generating highly enriched Th1/Tc1 and Th2/Tc2 functions are described. In particular, the generation of these functions are attained by the addition of an immune suppression drug, rapamycin or a rapamycin derivative compound. In addition to enhanced purity of T cell function, the T cells generated in rapamycin also express molecules that improve immune T cell function such as CD28 and CD62L. Such rapamycin generated functional T cell subsets may have application in the prevention or treatment of GVHD after allogeneic hematopoietic stem cell transplantation, the treatment of autoimmunity, or the therapy of infection or cancer.

摘要翻译： 描述了产生高度富集的Th1 / Tc1和Th2 / Tc2功能的方法。 特别地，通过添加免疫抑制药物雷帕霉素或雷帕霉素衍生物化合物来实现这些功能的产生。 除了提高T细胞功能的纯度之外，雷帕霉素中产生的T细胞还表达提高免疫T细胞功能如CD28和CD62L的分子。 这种雷帕霉素产生的功能性T细胞亚群可以用于预防或治疗同种异体造血干细胞移植后的GVHD，治疗自身免疫或治疗感染或癌症。

公开(公告)号：US20060062763A1

公开(公告)日：2006-03-23

申请号：US11226168

申请日：2005-09-14

申请人： Wayne Godfrey ,  Carl June

发明人： Wayne Godfrey ,  Carl June

IPC分类号： C12N5/08

CPC分类号： C12N5/0087 ,  C12N5/0636 ,  C12N2501/23 ,  C12N2501/505 ,  C12N2501/515 ,  C12N2501/599

摘要： The present invention encompasses methods, and kits for the isolation and expansion of T regulatory cells having the CD45RA+ phenotype, including such cells from human umbilical cord blood.

摘要翻译： 本发明包括用于分离和扩增具有CD45RA + +表型的T调节细胞的方法和试剂盒，包括来自人脐带血的这种细胞。

公开(公告)号：US07638325B2

公开(公告)日：2009-12-29

申请号：US10336224

申请日：2003-01-03

申请人： Carl June ,  James Riley ,  Marcela Maus ,  Anna Thomas

发明人： Carl June ,  James Riley ,  Marcela Maus ,  Anna Thomas

IPC分类号： C12N5/02 ,  C12N5/06 ,  C12N15/00 ,  C12N15/63 ,  C07K16/00

CPC分类号： C12N5/0636 ,  A61K2039/5158 ,  C12N5/0634 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599 ,  C12N2502/99 ,  C12N2510/00

摘要： The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells using an engineered multivalent signaling platform. Compositions of cells activated and expanded by the methods herein are further provided.

摘要翻译： 本发明一般涉及用于激活和扩增细胞的方法，更具体地，涉及使用工程化多价信号平台来激活和/或刺激细胞的新方法。 进一步提供通过本文方法激活和扩增的细胞的组成。

公开(公告)号：US20060204500A1

公开(公告)日：2006-09-14

申请号：US11373777

申请日：2006-03-10

申请人： Carl June ,  Richard Carroll ,  James Riley ,  Daniel St. Louis ,  Bruce Levine

发明人： Carl June ,  Richard Carroll ,  James Riley ,  Daniel St. Louis ,  Bruce Levine

IPC分类号： A61K39/42

CPC分类号： C07K16/2818

摘要： The invention relates to a method for modulating HIV-1 fusion cofactor expression by manipulating CTLA-4 on the surface of T cells. The invention encompasses methods for modulating HIV-1 fusion cofactor expression by inhibiting one or more intracellular signals resulting from stimulation of CTLA-4. In one embodiment, expression of the HIV-1 fusion cofactor, CCR5, is downregulated by binding of anti-CTLA-4 antibody to CTLA-4.

摘要翻译： 本发明涉及通过在T细胞表面上操作CTLA-4来调节HIV-1融合辅因子表达的方法。 本发明包括通过抑制由刺激CTLA-4引起的一种或多种细胞内信号来调节HIV-1融合辅因子表达的方法。 在一个实施方案中，HIV-1融合辅因子CCR5的表达通过抗CTLA-4抗体与CTLA-4的结合被下调。