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17 条记录 (耗时 0.035 秒)

    System and method for fluorescence monitoring
    1.
    发明授权
    System and method for fluorescence monitoring 失效
    荧光监测系统和方法

    公开(公告)号：US07081226B1

    公开(公告)日：2006-07-25

    申请号：US08869275

    申请日：1997-06-04

    申请人： Carl T. Wittwer ,  Kirk M. Ririe ,  Randy P. Rasmussen ,  David R. Hillyard

    发明人： Carl T. Wittwer ,  Kirk M. Ririe ,  Randy P. Rasmussen ,  David R. Hillyard

    IPC分类号： G01N33/50 ,  C12P19/34

    CPC分类号： B01L7/52 ,  B01L3/5025 ,  B01L2300/0803 ,  B01L2300/0838 ,  B01L2300/1844 ,  B01L2400/0406 ,  B01L2400/0409

    摘要： A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Monitoring of the DNA amplification is monitored by fluorescence once per cycle or many times per cycle. The present invention provides that fluorescence monitoring of PCR is a powerful tool for DNA quantification.

    摘要翻译： 公开了一种热循环方法和装置。 该装置包括样品室，其温度可以在进行许多生物学过程（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调制。 将生物样品置于玻璃微毛细管中，然后置于样品室内。 可编程控制器调节样品室内样品的温度。 DNA扩增的监测通过每周期荧光一次或每次循环多次监测。 本发明提供PCR的荧光监测是DNA定量的有力工具。

    Rapid thermal cycling device
    9.
    发明授权
    Rapid thermal cycling device 失效
    快速热循环装置

    公开(公告)号：US5455175A

    公开(公告)日：1995-10-03

    申请号：US179969

    申请日：1994-01-10

    申请人： Carl T. Wittwer ,  David R. Hillyard ,  Kirk M. Ririe

    发明人： Carl T. Wittwer ,  David R. Hillyard ,  Kirk M. Ririe

    IPC分类号： B01L7/00 ,  C12M1/38 ,  G01N30/02 ,  G01N30/06 ,  G01N30/30 ,  G01N30/46 ,  C12M1/34

    CPC分类号： B01L7/52 ,  G01N2030/3084 ,  Y10S435/809

    摘要： A thermal cycling device having a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are placed in glass microcapillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achievable by the present invention.

    摘要翻译： 具有样品室的热循环装置，其温度可以在进行许多生物学过程（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调制。 将生物样品置于玻璃微毛细管中，然后置于样品室内。 可编程控制器调节样品室内样品的温度。 一旦加热循环完成，控制器打开一个通向室的门，用于排出热空气并冷却环境空气进入。对应于扩增DNA的最佳变性，退火和伸长温度的温度对时间曲线可由本发明实现 发明。