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公开(公告)号：US07081226B1
公开(公告)日：2006-07-25
申请号：US08869275
申请日：1997-06-04
CPC分类号： B01L7/52 , B01L3/5025 , B01L2300/0803 , B01L2300/0838 , B01L2300/1844 , B01L2400/0406 , B01L2400/0409
摘要： A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Monitoring of the DNA amplification is monitored by fluorescence once per cycle or many times per cycle. The present invention provides that fluorescence monitoring of PCR is a powerful tool for DNA quantification.
摘要翻译： 公开了一种热循环方法和装置。 该装置包括样品室，其温度可以在进行许多生物学过程（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调制。 将生物样品置于玻璃微毛细管中，然后置于样品室内。 可编程控制器调节样品室内样品的温度。 DNA扩增的监测通过每周期荧光一次或每次循环多次监测。 本发明提供PCR的荧光监测是DNA定量的有力工具。
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公开(公告)号：US20090311673A1
公开(公告)日：2009-12-17
申请号：US11931178
申请日：2007-10-31
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
摘要翻译： 本发明涉及用于进行PCR和监测包含核酸和荧光染料的样品的反应的装置。 示例性装置包括用于加热和冷却样品的热交换部件，用于重复操作热交换部件以使样品经受热循环的控制装置，用于光学激发样品以引起样品发荧光的激发源， 检测来自样品的温度依赖性荧光水平，以及被配置为记录和处理来自荧光染料的发射的处理器。
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3.发明授权PCR method for nucleic acid quantification utilizing second or third order rate constants 有权利用二阶或三阶速率常数进行核酸定量的PCR方法公开(公告)号：US06232079B1
公开(公告)日：2001-05-15
申请号：US09635344
申请日：2000-08-09
IPC分类号： C12Q168
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to a method of determining the concentration of a nucleic acid product that had been amplified through polymerase chain reaction (PCR). More particularly, the present invention relates to a method wherein a rate constant is determined for a known concentration of amplified product by monitoring the rate of hybridization of the known concentration, and then the concentration of an unknown concentration of a nucleic acid product can be determined by determining the rate of annealing for the unknown concentration, and calculating the concentration from the rate of annealing and the rate constant.
摘要翻译： 本发明涉及通过聚合酶链式反应（PCR）扩增的核酸产物的浓度测定方法。 更具体地，本发明涉及一种方法，其中通过监测已知浓度的杂交速率确定已知浓度的扩增产物的速率常数，然后可以确定核酸产物的未知浓度的浓度 通过确定未知浓度的退火速率，并从退火速率和速率常数计算浓度。
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公开(公告)号：US20120258524A1
公开(公告)日：2012-10-11
申请号：US13465364
申请日：2012-05-07
IPC分类号： C12M1/34
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
摘要翻译： 本发明涉及用于进行PCR和监测包含核酸和荧光染料的样品的反应的装置。 示例性装置包括用于加热和冷却样品的热交换部件，用于重复操作热交换部件以使样品经受热循环的控制装置，用于光学激发样品以引起样品发荧光的激发源， 检测来自样品的温度依赖性荧光水平，以及被配置为记录和处理来自荧光染料的发射的处理器。
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公开(公告)号：US08343754B2
公开(公告)日：2013-01-01
申请号：US13465364
申请日：2012-05-07
IPC分类号： C12M1/00
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
摘要翻译： 本发明涉及用于进行PCR和监测包含核酸和荧光染料的样品的反应的装置。 示例性装置包括用于加热和冷却样品的热交换部件，用于重复操作热交换部件以使样品经受热循环的控制装置，用于光学激发样品以引起样品发荧光的激发源， 检测来自样品的温度依赖性荧光水平，以及被配置为记录和处理来自荧光染料的发射的处理器。
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公开(公告)号：US20090258414A1
公开(公告)日：2009-10-15
申请号：US11926775
申请日：2007-10-29
IPC分类号： C12M1/00
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
摘要翻译： 本发明涉及用于进行PCR和监测包含核酸和荧光染料的样品的反应的装置。 示例性装置包括用于加热和冷却样品的热交换部件，用于重复操作热交换部件以使样品经受热循环的控制装置，用于光学激发样品以引起样品发荧光的激发源， 检测来自样品的温度依赖性荧光水平，以及被配置为记录和处理来自荧光染料的发射的处理器。
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公开(公告)号：US06569627B2
公开(公告)日：2003-05-27
申请号：US09799160
申请日：2001-03-05
IPC分类号： C12Q168
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to a method and kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to a method wherein the nucleic acid is monitored during polymerase chain reaction using a double-stranded nucleic acid binding dye capable of producing a fluorescent signal related to the amount of the nucleic acid present in a sample, wherien the dye is selected from the group consisting of SYBR™ Green I and pico green.
摘要翻译： 本发明涉及用于在扩增过程中监测核酸的方法和试剂盒。 更具体地说，本发明涉及一种方法，其中使用能够产生与样品中存在的核酸量相关的荧光信号的双链核酸结合染料在聚合酶链反应期间监测核酸， 染料选自SYBR TM Green I和微微绿。
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公开(公告)号：US06174670B1
公开(公告)日：2001-01-16
申请号：US08869276
申请日：1997-06-04
IPC分类号： C12Q168
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： Methods of monitoring hybridization during polymerase chain reaction are disclosed. These methods are achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes. A fluorescence resonance energy transfer pair comprises fluorescein and Cy5 or Cy5.5. Methods for quantitating amplified DNA and determining its purity are carried out by analysis of melting and reannealing curves.
摘要翻译： 公开了在聚合酶链反应期间监测杂交的方法。 这些方法通过快速热循环和使用双链DNA染料或特异性杂交探针来实现。 荧光共振能量转移对包含荧光素和Cy5或Cy5.5。 通过分析熔解和再退火曲线进行定量扩增DNA并测定其纯度的方法。
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公开(公告)号：US08394608B2
公开(公告)日：2013-03-12
申请号：US11913120
申请日：2006-05-08
申请人： Kirk M. Ririe , Michael R. Newswander , Randy P. Rasmussen , Mark Aaron Poritz , Stewart Benjamin Smith , David E. Jones , Gary Clark Kessler
发明人： Kirk M. Ririe , Michael R. Newswander , Randy P. Rasmussen , Mark Aaron Poritz , Stewart Benjamin Smith , David E. Jones , Gary Clark Kessler
IPC分类号： C12P19/34
CPC分类号： B01L7/52 , B01L3/502723 , B01L3/50273 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0481 , B01L2400/049 , B01L2400/0644 , B01L2400/065 , B01L2400/0655 , B01L2400/0677 , B01L2400/0683 , C12Q1/6844
摘要： Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analysis include nucleic acid amplification and detection and immuno-PCR.
摘要翻译： 提供设备，容器和方法用于在封闭环境中进行生物分析。 说明性的生物学分析包括核酸扩增和检测和免疫PCR。
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公开(公告)号：US20100105029A1
公开(公告)日：2010-04-29
申请号：US11913120
申请日：2006-05-08
申请人： Kirk M. Ririe , Michael R. Newswander , Randy P. Rasmussen , Mark Aaron Poritz , Stewart Benjamin Smith , David E. Jones , Gary Clark Kessler
发明人： Kirk M. Ririe , Michael R. Newswander , Randy P. Rasmussen , Mark Aaron Poritz , Stewart Benjamin Smith , David E. Jones , Gary Clark Kessler
CPC分类号： B01L7/52 , B01L3/502723 , B01L3/50273 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0481 , B01L2400/049 , B01L2400/0644 , B01L2400/065 , B01L2400/0655 , B01L2400/0677 , B01L2400/0683 , C12Q1/6844
摘要： Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.
摘要翻译： 提供设备，容器和方法用于在封闭环境中进行生物分析。 说明性的生物学分析包括核酸扩增和检测和免疫PCR。
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