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    Neomorphogenesis of cartilage in vivo from cell culture
    4.
    发明授权
    Neomorphogenesis of cartilage in vivo from cell culture 失效
    细胞培养体内软骨的新形态

    公开(公告)号：US5041138A

    公开(公告)日：1991-08-20

    申请号：US339155

    申请日：1989-04-17

    申请人： Joseph P. Vacanti ,  Charles A. Vacanti ,  Robert S. Langer

    发明人： Joseph P. Vacanti ,  Charles A. Vacanti ,  Robert S. Langer

    IPC分类号： A61F2/30 ,  A61F2/02 ,  A61F2/06 ,  A61K35/12 ,  A61L27/00 ,  A61L27/38 ,  A61L27/60 ,  C12N5/00 ,  C12N5/02 ,  C12N5/077

    CPC分类号： C12N5/0655 ,  A61F2/02 ,  A61F2/062 ,  A61L27/38 ,  A61L27/60 ,  C12N5/0068 ,  A61F2/30756 ,  A61F2002/30062 ,  A61F2210/0004 ,  A61K35/12 ,  A61L2430/06 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40 ,  Y10S530/84 ,  Y10S623/919

    摘要： Methods and artificial matrices for the growth and implantation of cartilaginous structures and surfaces are disclosed. In the preferred embodiments, chondrocytes are grown on biodegradable, biocompatible fibrous polymeric matrices. Optionally, the cells are proliferated in vitro until an adequate cell volume and density has developed for the cells to survive and proliferate in vivo. One advantage of the matrices is that they can be cast or molded into a desired shape, on an individual basis, so that the final product closely resembles a patient's own ear or nose. Alternatively, flexible matrices can be used which can be manipulated at the time of implantation, as in a joint, followed by remodeling through cell growth and proliferation in vivo. The cultured cells can also be maintained on the matrix in a nutrient media for production of bioactive molecules such as angiogenesis inhibiting factor.

    摘要翻译： 公开了用于生长和植入软骨结构和表面的方法和人造基质。 在优选的实施方案中，软骨细胞生长在可生物降解的生物相容的纤维聚合物基质上。 任选地，细胞在体外增殖，直至形成足够的细胞体积和密度，使细胞在体内存活和增殖。 基质的一个优点是它们可以在个体的基础上铸造或模制成所需的形状，使得最终产品非常类似于患者自己的耳朵或鼻子。 或者，可以使用可在植入时操作的柔性基质，如在关节中，随后通过体内细胞生长和增殖重塑。 培养的细胞也可以在营养培养基中维持在基质上，用于生产生物活性分子如血管生成抑制因子。

    Biodegradable synthetic polymeric fibrous matrix containing chondrocyte for in vivo production of a cartilaginous structure
    5.
    发明授权
    Biodegradable synthetic polymeric fibrous matrix containing chondrocyte for in vivo production of a cartilaginous structure 失效
    含有软骨细胞的可生物降解的合成聚合物纤维基质用于体内产生软骨结构

    公开(公告)号：US5736372A

    公开(公告)日：1998-04-07

    申请号：US509952

    申请日：1990-04-16

    申请人： Joseph P. Vacanti ,  Charles A. Vacanti ,  Robert S. Langer

    发明人： Joseph P. Vacanti ,  Charles A. Vacanti ,  Robert S. Langer

    IPC分类号： A61F2/02 ,  A61F2/06 ,  A61F2/30 ,  A61K35/12 ,  A61L27/38 ,  A61L27/60 ,  C12N5/00 ,  C12N5/077 ,  C12N11/08 ,  A61F2/28 ,  A61F2/18

    CPC分类号： C12N5/0655 ,  A61F2/02 ,  A61F2/062 ,  A61L27/38 ,  A61L27/60 ,  C12N5/0068 ,  A61F2/30756 ,  A61F2002/30062 ,  A61F2210/0004 ,  A61K35/12 ,  A61L2430/06 ,  C12N2533/18 ,  C12N2533/30 ,  C12N2533/40

    摘要： Methods and artificial matrices for the growth and implantation of cartilaginous structures and surfaces and bone are disclosed. In the preferred embodiments, chondrocytes are grown on biodegradable, biocompatible fibrous polymeric matrices. Optionally, the cells are proliferated in vitro until an adequate cell volume and density has developed for the cells to survive and proliferate in vivo. One advantage of the matrices is that they can be cast or molded into a desired shape, on an individual basis, so that the final product closely resembles a patient's own ear or nose. Alternatively, flexible matrices can be used which can be manipulated at the time of implantation, as in a joint, followed by remodeling through cell growth and proliferation in vivo. The cultured cells can also be maintained on the matrix in a nutrient media for production of bioactive molecules such as angiogenesis inhibiting factor. Examples are provided showing the growth of hyaline cartilage for joint relinings, the growth of elastic cartilage for plastic or reconstructive replacement of cartilage structures, and repair of large bone defects.

    摘要翻译： 公开了用于生长和植入软骨结构和表面和骨的方法和人造基质。 在优选的实施方案中，软骨细胞生长在可生物降解的生物相容的纤维聚合物基质上。 任选地，细胞在体外增殖，直至形成足够的细胞体积和密度，使细胞在体内存活和增殖。 基质的一个优点是它们可以在个体的基础上铸造或模制成所需的形状，使得最终产品非常类似于患者自己的耳朵或鼻子。 或者，可以使用可在植入时操作的柔性基质，如在关节中，随后通过体内细胞生长和增殖重塑。 培养的细胞也可以在营养培养基中维持在基质上，用于生产生物活性分子如血管生成抑制因子。 提供了示例，其显示用于联合放置的透明软骨的生长，塑料的弹性软骨的生长或软骨结构的重建置换以及大的骨缺损的修复。

    Implantation of cell-matrix structure adjacent mesentery, omentum or peritoneum tissue
    8.
    发明授权
    Implantation of cell-matrix structure adjacent mesentery, omentum or peritoneum tissue 失效
    植入细胞基质结构邻近肠系膜，网膜或腹膜组织

    公开(公告)号：US5804178A

    公开(公告)日：1998-09-08

    申请号：US203509

    申请日：1994-02-28

    申请人： Joseph P. Vacanti ,  Robert S. Langer ,  Lynt Johnson

    发明人： Joseph P. Vacanti ,  Robert S. Langer ,  Lynt Johnson

    IPC分类号： A61L27/36 ,  A61L27/38 ,  C12N5/00 ,  C12N11/08 ,  A61F2/18 ,  A61F2/28

    CPC分类号： A61L27/3839 ,  A61L27/3843 ,  A61L27/3869 ,  C12N5/0068 ,  A61L2430/06 ,  C12N2501/18 ,  C12N2533/30 ,  C12N2533/40

    摘要： A matrix structure containing attached cells such as endocrine cells, fibroblasts, endothelial cells or genitourinary cells is implanted in a patient adjacent tissue having a high surface area and vasculature such as mesentery, omentum or peritoneum tissue. Large volumes of cells can be attached to the matrix and the matrix implanted with minimum trauma and blood loss into a patient to produce a functional organ equivalent. Multiple matrix structures containing cells can be implanted to functionally resemble naturally occurring organs. Implanting multiple matrices between folds of the mesentery is particularly well suited for growth of endocrine structures, including liver, pancreas, and adrenal gland. The matrix structure is preferably formed from a biodegradable artificial polymer. Collagen and non-biodegradable materials can also be used, and the matrix structure can be overlaid with a material that enhances cell attachment. Materials such as angiogenesis factors can be incorporated into a matrix and implanted prior to implanting the matrix containing cells or the materials can be incorporated into the matrix containing cells. Cells attached to the matrix may be cultured in vitro prior to implanting. Matrix structures containing different types of cells can be implanted juxtapositioned with each other.

    摘要翻译： 将包含附着细胞如内分泌细胞，成纤维细胞，内皮细胞或泌尿生殖细胞的基质结构植入具有高表面积和脉管系统如肠系膜，网膜或腹膜组织的邻近组织中。 大量的细胞可以连接到基质上，并且基质以最小的创伤和失血植入患者体内以产生功能性器官当量。 可以将包含细胞的多个基质结构植入功能上类似天然存在的器官。 在肠系膜的折叠之间植入多个基质特别适用于内分泌结构的生长，包括肝，胰腺和肾上腺。 基质结构优选由生物可降解的人造聚合物形成。 还可以使用胶原蛋白和不可生物降解的材料，并且可以用增强细胞附着的材料覆盖基质结构。 诸如血管生成因子的材料可以掺入基质中并在植入包含细胞的基质之前植入，或者该材料可以并入含有基质的细胞中。 附着于基质的细胞可以在植入前在体外培养。 含有不同类型细胞的基质结构可以彼此并置。