公开(公告)号：US6153880A

公开(公告)日：2000-11-28

申请号：US410379

申请日：1999-09-30

申请人： Charles William Russ, IV ,  Jerry T. Dowell ,  Steven M. Fischer

发明人： Charles William Russ, IV ,  Jerry T. Dowell ,  Steven M. Fischer

IPC分类号： H01J49/42

CPC分类号： H01J49/4215 ,  H01J49/067

摘要： A mass spectrometer method and apparatus improve resolution and sensitivity. A voltage supply is coupled to a mass filter for applying a radio frequency voltage and for operating at at least one selected frequency. An ion optical element such as an entrance lens is driven by a voltage supply that is phase coherent with the voltage applied to the mass filter. The ion beam is tailored so that the phase space relationship of the ions is more closely matched to the acceptance of the mass filter. The ions in the incoming beam are dynamically matched to the acceptance of the mass filter over each cycle of the voltage applied to the mass filter. The ion optical element may be a single entrance lens to which is applied only a single phase coherent voltage.

摘要翻译： 质谱仪方法和设备提高分辨率和灵敏度。 电压源耦合到质量过滤器以施加射频电压并且以至少一个所选择的频率进行操作。 诸如入射透镜的离子光学元件由与施加到质量过滤器的电压相位相干的电压源驱动。 定制离子束，使得离子的相位空间关系与质量过滤器的接受度更接近。 入射束中的离子与施加到质量过滤器的电压的每个周期动态地匹配质量过滤器的接受。 离子光学元件可以是仅施加单相相干电压的单个入射透镜。

公开(公告)号：US20120108448A1

公开(公告)日：2012-05-03

申请号：US12938953

申请日：2010-11-03

申请人： Frank E. Kuhlmann ,  Heloise Logan ,  Steven M. Fischer ,  Xiangdong Don Li

发明人： Frank E. Kuhlmann ,  Heloise Logan ,  Steven M. Fischer ,  Xiangdong Don Li

IPC分类号： C40B30/02 ,  G06F19/00

CPC分类号： H01J49/0036 ,  G16C20/20 ,  G16C20/90

摘要： Systems and method for curation of mass spectral libraries. In general, the systems and methods provided herein (a) obtain an experimentally derived mass spectrum of a compound of interest; (b) identify a peak in the mass spectrum that represent an experimental m/z value for an ion fragment of the compound of interest; (c) remove from the mass spectrum any peak that does not correspond to the compound of interest; and (d) replace the experimental m/z value for the peak identified in step (b) with a calculated theoretical m/z value for the ion fragment.

摘要翻译： 用于质谱图谱库的系统和方法。 通常，本文提供的体系和方法（a）获得目的化合物的实验衍生的质谱; （b）鉴定质谱中的峰，其代表感兴趣化合物的离子片段的实验m / z值; （c）从质谱中除去与目的化合物不符的峰; 和（d）用离子片段的计算理论m / z值代替步骤（b）中鉴定的峰的实验m / z值。

公开(公告)号：US07422865B2

公开(公告)日：2008-09-09

申请号：US10341310

申请日：2003-01-13

申请人： Steven M. Fischer

发明人： Steven M. Fischer

IPC分类号： C12Q1/37 ,  C07K1/00 ,  C07K17/06 ,  C07K17/08

CPC分类号： G01N33/6803 ,  C07K1/36 ,  C12Q1/37 ,  G01N33/6848 ,  Y10S530/815 ,  Y10S530/816

摘要： The present invention provides a new and improved method for reducing the complexity of a proteomic sample, and preferably also for allowing identification of proteins in the sample. In one aspect, the invention provides a highly efficient method for identifying proteins in a proteomic sample by characterizing a single N-terminal peptide per protein. In another aspect, the invention provides a method for identifying proteins in a proteomic sample by characterizing a single C-terminal peptide per protein. In another aspect, the present invention provides a method for quantitative determination of differential protein expression and/or modification in different samples. In another aspect, the invention relates to kits useful for conveniently performing a method in accordance with the invention.

摘要翻译： 本发明提供了用于降低蛋白质组样品的复杂性的新的和改进的方法，并且优选还允许鉴定样品中的蛋白质。 在一个方面，本发明提供了通过对每个蛋白质表征单个N-末端肽来鉴定蛋白质组样品中的蛋白质的高效方法。 另一方面，本发明提供了通过对每个蛋白质表征单个C末端肽来鉴定蛋白质组样品中的蛋白质的方法。 另一方面，本发明提供了用于定量测定不同样品中差异蛋白表达和/或修饰的方法。 另一方面，本发明涉及可用于方便地执行根据本发明的方法的试剂盒。

公开(公告)号：US20080026479A1

公开(公告)日：2008-01-31

申请号：US11494383

申请日：2006-07-27

申请人： Patrick D. Perkins ,  Steven M. Fischer

发明人： Patrick D. Perkins ,  Steven M. Fischer

IPC分类号： G01N33/00

CPC分类号： G01N33/6821 ,  G01N33/6848 ,  Y10T436/24

摘要： A method of facilitating analysis of a peptide in a mass spectrometer comprising derivatizing the C-terminus of the peptide with an amino acid residue via a reaction with a carbodiimide reagent, yielding a derivative peptide, ionizing the derivative peptide with a double charge, and fragmenting the ionized derivative peptide in a mass spectrometry system, wherein binary fragments of the ionized derivative each include a charge, facilitating sequence analysis of the peptide.

摘要翻译： 一种促进质谱分析肽的方法，包括通过与碳二亚胺试剂的反应将肽的C末端与氨基酸残基衍生化，产生衍生肽，以双电荷离子化衍生肽，并将其断裂 质谱系统中的离子化衍生肽，其中电离衍生物的二元片段各自包含电荷，便于肽的序列分析。

公开(公告)号：US06849847B1

公开(公告)日：2005-02-01

申请号：US09146817

申请日：1998-09-04

申请人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： H01J49/04 ,  H01J49/16 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

摘要翻译： 公开了具有在环境压力下操作的基质辅助激光解吸电离（MALDI）源的质谱仪。 公开了该装置和方法以使用矩阵辅助激光解吸电离（MALDI）分析至少一个含有至少一种分析物的样品，该装置包括：本发明涉及用于将至少一种分析物离子化的装置和方法 用于递送到质量分析装置的样品，包括：（a）电离外壳，其包括经配置以将离子递送到质量分析装置的通道;（b）将所述电离外壳保持在大于100mTorr的环境压力 （c）保持器，其构造成用于在所述环境压力下将包含所述样品的基质保持在电离外壳中;（d）激光能量源，包括与电离外壳相关联的装置，用于将激光能量引导到由保持器保持的所述基体上 在环境压力下解吸并离子化样品中的至少一部分分析物，和（e）用于引导至少一个i的至少一部分的装置 通入分析物。 环境压力（AP-MALDI）源与各种质量分析仪兼容，特别是与质谱仪兼容，解决了与在真空下运行的常规MALDI源有关的许多问题。 描述了大气压力MALDI。 描述了有机分子或其片段，特别是生物分子，例如生物聚合物和生物体的分析。

公开(公告)号：US06486469B1

公开(公告)日：2002-11-26

申请号：US09429063

申请日：1999-10-29

申请人： Steven M. Fischer ,  Robert K. Crawford ,  Charles W. Russ, IV

发明人： Steven M. Fischer ,  Robert K. Crawford ,  Charles W. Russ, IV

IPC分类号： B01D5944

CPC分类号： H01J49/04

摘要： For delivery of ions from a higher pressure ion source to a mass analyzer operating at high vacuum, high pass ion filtration is effected within a dielectric capillary interface between the higher pressure ionization chamber and the lower pressure environment of a mass analyzer, by application of electrical potentials to end electrodes and to at least one electrode associated with the dielectric capillary between the ends, to create an end-to-end electric field generally opposing gas flow-assisted movement of ions from the upstream end to the downstream end, and to create a steeper voltage gradient along an upstream portion than along a downstream portion of the capillary. The voltage gradient along the steeper upstream portion of the capillary is sufficiently steep to cause ions having drift velocities below a lower limit to stall within the capillary. The respective potentials may be adjusted to increase the steepness of the upstream voltage gradient to increase the drift velocity lower limit.

摘要翻译： 为了将离子从较高压力的离子源输送到在高真空下操作的质量分析仪，在高压离子化室和质量分析仪的较低压力环境之间的介电毛细管界面内，通过施加电 将端电极和与末端之间的介电毛细管相关联的至少一个电极的电位产生一个端对端电场，通常相对于从上游端到下游端的离子的气流辅助运动，并产生 沿着上游部分比沿着毛细管的下游部分更陡峭的电压梯度。 沿着毛细管的较陡的上游部分的电压梯度足够陡峭，导致具有低于下限的漂移速度的离子在毛细管内停止。 可以调节各个电位以增加上游电压梯度的陡度以增加漂移速度下限。

公开(公告)号：US06359275B1

公开(公告)日：2002-03-19

申请号：US09352467

申请日：1999-07-14

申请人： James L. Bertsch ,  Steven M. Fischer ,  Julie B. Riccomini

发明人： James L. Bertsch ,  Steven M. Fischer ,  Julie B. Riccomini

IPC分类号： H01J4904

CPC分类号： H01J49/0404

摘要： A conduit for conducting ions from a high pressure ion source to a mass analyzer in mass spectrometry apparatus includes a capillary tube in which the lumenal surface of the bore near at least one end is a surface of an electrically conductive material. In one embodiment the conduit is constructed of a dielectric material and has an electrically conductive coating on an end portion of the lumenal surface.

摘要翻译： 用于将离子从高压离子源引导到质谱分析仪的质谱分析仪的导管包括毛细管，其中在至少一端附近的孔的内腔表面是导电材料的表面。 在一个实施例中，导管由电介质材料构成，并且在腔表面的端部上具有导电涂层。

公开(公告)号：USD334958S

公开(公告)日：1993-04-20

申请号：US567557

申请日：1990-08-15

申请人： Steven M. Fischer

设计人： Steven M. Fischer

公开(公告)号：US08507285B2

公开(公告)日：2013-08-13

申请号：US10388088

申请日：2003-03-13

申请人： Dean R. Thompson ,  Steven M. Fischer

发明人： Dean R. Thompson ,  Steven M. Fischer

IPC分类号： G01N33/00

CPC分类号： G01N33/6848 ,  G01N30/72 ,  H01J49/0045 ,  Y10T436/143333 ,  Y10T436/24

摘要： A method of identifying a biopolymer in a sample that includes one or more biopolymers. The biopolymers may be polypeptides, polynucleotides, or polysaccharides. The method makes use of mass spectral datasets. A first dataset includes measured masses of the one or more biopolymers that are in the sample. A second dataset includes measured masses of a collection of fragments of the one or more biopolymers. The method selects a mass from the first dataset and then matches masses from the second dataset with the selected mass. The matched masses represent fragments of the biopolymer with the selected mass. Once the masses in the second dataset have been matched they are compared to determine a monomer sequence for the biopolymer with the selected mass. The method may be repeated with additional masses in the first dataset.

摘要翻译： 鉴定样品中包含一种或多种生物聚合物的生物聚合物的方法。 生物聚合物可以是多肽，多核苷酸或多糖。 该方法利用质谱数据集。 第一数据集包括样品中一种或多种生物聚合物的测量质量。 第二数据集包括所述一种或多种生物聚合物的片段的集合的测量质量。 该方法从第一个数据集中选择一个质量，然后从具有所选质量的第二个数据集中匹配质量。 匹配的质量表示具有所选质量的生物聚合物的片段。 一旦第二数据集中的质量已经匹配，就比较它们以确定具有所选质量的生物聚合物的单体序列。 该方法可以在第一数据集中以附加质量重复。

公开(公告)号：US20080014603A1

公开(公告)日：2008-01-17

申请号：US11484912

申请日：2006-07-12

申请人： Steven M. Fischer ,  Christine A. Miller

发明人： Steven M. Fischer ,  Christine A. Miller

IPC分类号： C12Q1/37

CPC分类号： G01N33/6848 ,  B01L3/5027 ,  G01N27/447 ,  G01N30/7233 ,  G01N33/6851 ,  H01J49/04

摘要： A method of analyzing a sample comprising multiple protein species is provided. The proteins are separated by species such that the multiple protein species emerge in a sequential order and are then digested in the sequential order in which they emerge from the separation process. The digested proteins are introduced into a mass spectrometer in the same sequential order so that, within a given time window, the digested proteins introduced into the mass spectrometer are covariant.

摘要翻译： 提供了分析包含多种蛋白质种类的样品的方法。 蛋白质被物种分离，使得多个蛋白质物质以顺序排列出来，然后以从分离过程中出现的顺序消化。 将消化的蛋白质以相同的顺序引入质谱仪，使得在给定的时间窗口内，引入质谱仪的消化蛋白质是共变的。