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公开(公告)号：US5217878A
公开(公告)日：1993-06-08
申请号：US162184
申请日：1988-02-29
IPC分类号： C12N1/20 , C12N1/21 , C12N9/54 , C12N15/00 , C12N15/09 , C12N15/57 , C12N15/75 , C12N15/87 , C12N15/90 , C12R1/07
摘要： Novel methods and novel industrial Bacillus strains are provided for enhanced production of serine protease. Plasmid constructs comprising the serine protease gene are introduced into a compatible host, generally a Bacillus already overproducing serine protease. Preferred host cells are those from Bacillus novo sp. PB92 and the preferred serine protease gene also originates from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature sensitive origin of replication in the plasmid construct and growing under selective conditions.
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2.发明授权
公开(公告)号：US5733723A
公开(公告)日：1998-03-31
申请号：US295082
申请日：1994-08-24
IPC分类号： C12N1/20 , C12N1/21 , C12N9/28 , C12N9/54 , C12N15/00 , C12N15/09 , C12N15/55 , C12N15/67 , C12N15/75 , C12N15/90 , C12R1/07 , C12Q1/68 , C12N15/10
CPC分类号： C12N9/2417 , C12N15/67 , C12N15/75 , C12N15/90 , C12N9/54
摘要： Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce said polypeptide. Preferred host strains are Bacillus novo species PB92 which produces a high-alkaline proteolytic enzyme and B. licheniformis strain T5 which produces a thermostable .alpha.-amylase, and mutants and variants of said strains. Preferred polypeptide encoding genes are the protease encoding gene obtainable from Bacillus PB92 and the .alpha.-amylase encoding gene obtainable from B. licheniformis strain T5.
摘要翻译： 提供了包含稳定保持在其染色体中的DNA序列的两个或多个拷贝的转化的原核宿主，所述DNA序列包含编码感兴趣多肽的基因，其中所述拷贝通过内源染色体DNA序列分离。 还提供了用于产生所述转化的宿主菌株的方法。 与已经产生所述多肽的宿主菌株相比，转化的宿主菌株能够增加目的多肽的产量。 优选的宿主菌株是芽胞杆菌新种PB92，其产生高碱性蛋白水解酶和地衣芽孢杆菌菌株T5，其产生热稳定的α-淀粉酶，以及所述菌株的突变体和变体。 优选的编码多肽的多肽是可从芽孢杆菌PB92获得的蛋白酶编码基因和可从地衣芽孢杆菌菌株T5获得的α-淀粉酶编码基因。
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3.发明授权
公开(公告)号：US6124097A
公开(公告)日：2000-09-26
申请号：US049867
申请日：1998-03-27
IPC分类号： C12N1/20 , C12N1/21 , C12N9/28 , C12N9/54 , C12N15/00 , C12N15/09 , C12N15/55 , C12N15/67 , C12N15/75 , C12N15/90 , C12R1/07 , C12Q1/68 , C12P21/02
CPC分类号： C12N9/2417 , C12N15/67 , C12N15/75 , C12N15/90 , C12N9/54
摘要： Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce said polypeptide. Preferred host strains are Bacillus novo species PB92 which produces a high-alkaline proteolytic enzyme and B. licheniformis strain T5 which produces a thermostable .alpha.-amylase, and mutants and variants of said strains. Preferred poly-peptide encoding genes are the protease encoding gene obtainable from Bacillus PB92 and the .alpha.-amylase encoding gene obtainable from B. licheniformis strain T5.
摘要翻译： 提供了包含稳定保持在其染色体中的DNA序列的两个或多个拷贝的转化的原核宿主，所述DNA序列包含编码感兴趣多肽的基因，其中所述拷贝通过内源染色体DNA序列分离。 还提供了用于产生所述转化的宿主菌株的方法。 与已经产生所述多肽的宿主菌株相比，转化的宿主菌株能够增加目的多肽的产量。 优选的宿主菌株是芽胞杆菌新种PB92，其产生高碱性蛋白水解酶和地衣芽孢杆菌菌株T5，其产生热稳定的α-淀粉酶，以及所述菌株的突变体和变体。 优选的多肽编码基因是可从芽孢杆菌PB92获得的蛋白酶编码基因和可从地衣芽孢杆菌菌株T5获得的α-淀粉酶编码基因。
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4.发明授权Transformed industrial bacillus strains and methods for making and using them 失效转化的工业芽孢杆菌菌株及其制备和使用方法
公开(公告)号：US6083718A
公开(公告)日：2000-07-04
申请号：US965019
申请日：1997-11-05
申请人： Johan Pieter Marinus Sanders , Johannes Abel Van Den Berg , Peter Michael Andreoli , Yvonne Johanna Vos , Jan Hendrik Van Ee , Leo J. S. M. Mulleners
发明人： Johan Pieter Marinus Sanders , Johannes Abel Van Den Berg , Peter Michael Andreoli , Yvonne Johanna Vos , Jan Hendrik Van Ee , Leo J. S. M. Mulleners
CPC分类号： C12N9/2417 , C12N15/63 , C12N15/75 , C12N9/54 , C12N9/6481 , C12N9/6483 , C12Y304/23004
摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
摘要翻译： 提供新颖的方法和新型工业单细胞微生物菌株，特别是工业芽孢杆菌菌株，用于增强内源和外源多肽的产生。 将含有表达目的多肽的基因的克隆载体引入相容宿主体内。 选择通过将载体整合到宿主细胞染色体中，以稳定的方式携带引入载体的转化宿主。 实现含有目标基因的载体的有效转移，所得到的工业菌株转化体是所需多肽产物的有效稳定的生产者。
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5.发明授权Transformed industrial bacillus strains and methods for making and using them 失效转化的工业芽孢杆菌菌株及其制备和使用方法
公开(公告)号：US5624829A
公开(公告)日：1997-04-29
申请号：US993785
申请日：1992-12-21
申请人： Johan P. M. Sanders , Johannes A. van den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. van Ee , Leo J. S. M. Mulleners
发明人： Johan P. M. Sanders , Johannes A. van den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. van Ee , Leo J. S. M. Mulleners
CPC分类号： C12N9/2417 , C12N15/63 , C12N15/75 , C12N9/54 , C12N9/6481 , C12N9/6483 , C12Y304/23004
摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
摘要翻译： 提供新颖的方法和新型工业单细胞微生物菌株，特别是工业芽孢杆菌菌株，用于增强内源和外源多肽的产生。 将含有表达目的多肽的基因的克隆载体引入相容宿主体内。 选择通过将载体整合到宿主细胞染色体中，以稳定的方式携带引入载体的转化宿主。 实现含有目标基因的载体的有效转移，所得到的工业菌株转化体是所需多肽产物的有效稳定的生产者。
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公开(公告)号：US5716807A
公开(公告)日：1998-02-10
申请号：US698785
申请日：1996-08-16
申请人： Johan Pieter Marinus Sanders , Johannes Abel Van den Berg , Peter Michael Andreoli , Yvonne Johanna Vos , Jan Hendrik van Ee , Leo J. S. M. Mulleners
发明人： Johan Pieter Marinus Sanders , Johannes Abel Van den Berg , Peter Michael Andreoli , Yvonne Johanna Vos , Jan Hendrik van Ee , Leo J. S. M. Mulleners
CPC分类号： C12N9/2417 , C12N15/63 , C12N15/75 , C12N9/54 , C12N9/6481 , C12N9/6483 , C12Y304/23004
摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
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公开(公告)号：US5238833A
公开(公告)日：1993-08-24
申请号：US701596
申请日：1991-05-13
申请人： Johan P. M. Sanders , Johannes A. Van Den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. Van EE , Leo J. S. M. Mulleners
发明人： Johan P. M. Sanders , Johannes A. Van Den Berg , Peter M. Andreoli , Yvonne J. Vos , Jan H. Van EE , Leo J. S. M. Mulleners
CPC分类号： C12N9/2417 , C12N15/63 , C12N15/75 , C12N9/54 , C12N9/6481
摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
摘要翻译： 提供新颖的方法和新型工业单细胞微生物菌株，特别是工业芽孢杆菌菌株，用于增强内源和外源多肽的产生。 将含有表达目的多肽的基因的克隆载体引入相容宿主体内。 选择通过将载体整合入宿主细胞染色体以稳定方式携带引入载体的转化宿主实现了含有目的基因的载体的有效转移，所得到的工业菌株转化体是有效的，所需多肽的稳定生产者 产品。
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