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    Stable gene amplification in chromosomal DNA of prokaryotic microorganisms
    6.
    发明授权
    Stable gene amplification in chromosomal DNA of prokaryotic microorganisms 失效
    在原核生物的染色体DNA中稳定的基因扩增

    公开(公告)号：US5733723A

    公开(公告)日：1998-03-31

    申请号：US295082

    申请日：1994-08-24

    申请人： Christiaan A. G. van Eekelen ,  Johannes C. van der Laan ,  Leo J. S. M. Mulleners

    发明人： Christiaan A. G. van Eekelen ,  Johannes C. van der Laan ,  Leo J. S. M. Mulleners

    IPC分类号： C12N1/20 ,  C12N1/21 ,  C12N9/28 ,  C12N9/54 ,  C12N15/00 ,  C12N15/09 ,  C12N15/55 ,  C12N15/67 ,  C12N15/75 ,  C12N15/90 ,  C12R1/07 ,  C12Q1/68 ,  C12N15/10

    CPC分类号： C12N9/2417 ,  C12N15/67 ,  C12N15/75 ,  C12N15/90 ,  C12N9/54

    摘要： Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce said polypeptide. Preferred host strains are Bacillus novo species PB92 which produces a high-alkaline proteolytic enzyme and B. licheniformis strain T5 which produces a thermostable .alpha.-amylase, and mutants and variants of said strains. Preferred polypeptide encoding genes are the protease encoding gene obtainable from Bacillus PB92 and the .alpha.-amylase encoding gene obtainable from B. licheniformis strain T5.

    摘要翻译： 提供了包含稳定保持在其染色体中的DNA序列的两个或多个拷贝的转化的原核宿主，所述DNA序列包含编​​码感兴趣多肽的基因，其中所述拷贝通过内源染色体DNA序列分离。 还提供了用于产生所述转化的宿主菌株的方法。 与已经产生所述多肽的宿主菌株相比，转化的宿主菌株能够增加目的多肽的产量。 优选的宿主菌株是芽胞杆菌新种PB92，其产生高碱性蛋白水解酶和地衣芽孢杆菌菌株T5，其产生热稳定的α-淀粉酶，以及所述菌株的突变体和变体。 优选的编码多肽的多肽是可从芽孢杆菌PB92获得的蛋白酶编码基因和可从地衣芽孢杆菌菌株T5获得的α-淀粉酶编码基因。

    Stable gene amplification in chromosomal DNA of prokaryotic microorganisms
    7.
    发明授权
    Stable gene amplification in chromosomal DNA of prokaryotic microorganisms 失效
    在原核生物的染色体DNA中稳定的基因扩增

    公开(公告)号：US6124097A

    公开(公告)日：2000-09-26

    申请号：US049867

    申请日：1998-03-27

    申请人： Christiaan A. G. van Eekelen ,  Johannes C. Van Der Laan ,  Leo J. S. M. Mulleners

    发明人： Christiaan A. G. van Eekelen ,  Johannes C. Van Der Laan ,  Leo J. S. M. Mulleners

    IPC分类号： C12N1/20 ,  C12N1/21 ,  C12N9/28 ,  C12N9/54 ,  C12N15/00 ,  C12N15/09 ,  C12N15/55 ,  C12N15/67 ,  C12N15/75 ,  C12N15/90 ,  C12R1/07 ,  C12Q1/68 ,  C12P21/02

    CPC分类号： C12N9/2417 ,  C12N15/67 ,  C12N15/75 ,  C12N15/90 ,  C12N9/54

    摘要： Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce said polypeptide. Preferred host strains are Bacillus novo species PB92 which produces a high-alkaline proteolytic enzyme and B. licheniformis strain T5 which produces a thermostable .alpha.-amylase, and mutants and variants of said strains. Preferred poly-peptide encoding genes are the protease encoding gene obtainable from Bacillus PB92 and the .alpha.-amylase encoding gene obtainable from B. licheniformis strain T5.

    摘要翻译： 提供了包含稳定保持在其染色体中的DNA序列的两个或多个拷贝的转化的原核宿主，所述DNA序列包含编​​码感兴趣多肽的基因，其中所述拷贝通过内源染色体DNA序列分离。 还提供了用于产生所述转化的宿主菌株的方法。 与已经产生所述多肽的宿主菌株相比，转化的宿主菌株能够增加目的多肽的产量。 优选的宿主菌株是芽胞杆菌新种PB92，其产生高碱性蛋白水解酶和地衣芽孢杆菌菌株T5，其产生热稳定的α-淀粉酶，以及所述菌株的突变体和变体。 优选的多肽编码基因是可从芽孢杆菌PB92获得的蛋白酶编码基因和可从地衣芽孢杆菌菌株T5获得的α-淀粉酶编码基因。