HELICASE DEPENDENT ISOTHERMAL AMPLIFICATION USING NICKING ENZYMES
    10.
    发明申请
    HELICASE DEPENDENT ISOTHERMAL AMPLIFICATION USING NICKING ENZYMES 有权
    使用镍化合物进行依赖相同的等温放大

    公开(公告)号:US20130210019A1

    公开(公告)日:2013-08-15

    申请号:US13817174

    申请日:2011-08-16

    IPC分类号: C12Q1/68

    摘要: The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.

    摘要翻译: 本发明涉及用于扩增模板核酸的方法,其中所述方法包括在切口内切核酸酶存在下使用解旋酶依赖性扩增(HDA)反应扩增所述模板核酸,并且其中所述模板核酸包含识别的序列 通过所述切口内切核酸酶或由所述切口内切核酸酶识别的序列在HDA反应期间引入模板核酸。 本发明还涉及用于扩增核酸的试剂盒,其包含切口内切核酸酶,解旋酶和DNA聚合酶。