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公开(公告)号：US20100222267A1
公开(公告)日：2010-09-02
申请号：US12664454
申请日：2008-06-13
摘要： The present invention provides a recombinant protein comprising the sequence of a transferrin mutant, wherein Ser415 is mutated to an amino acid which does not allow glycosylation at Asn413 and/or wherein Thr613 is mutated to an amino acid which does not allow glycosylation as Asn611. It also provides polynucleotides encoding the same and methods of making and using said recombinant protein.
摘要翻译： 本发明提供了包含转铁蛋白突变体序列的重组蛋白，其中Ser415突变为不允许Asn413上的糖基化和/或其中Thr613突变为不允许糖基化作为Asn611的氨基酸的氨基酸。 它还提供了编码其的多核苷酸以及制备和使用所述重组蛋白的方法。
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公开(公告)号：US08969064B2
公开(公告)日：2015-03-03
申请号：US13474313
申请日：2012-05-17
IPC分类号： C12N1/00 , C12P21/02 , C12P21/06 , C12N15/64 , C12N15/66 , C12N15/00 , C12N9/90 , C07K14/395 , C07K14/79 , C12N15/81
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
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公开(公告)号：US08252551B2
公开(公告)日：2012-08-28
申请号：US10584486
申请日：2004-12-23
IPC分类号： C12P21/02
CPC分类号： C12N15/81
摘要： The present invention provides a 2 μm-family plasmid comprising a polynucleotide sequence insertion, deletion and/or substitution between the first base after the last functional codon of at least one of either a REP2 gene or an FLP gene and the last base before the FRT site in an inverted repeat adjacent to said gene.
摘要翻译： 本发明提供了包含在REP2基因或FLP基因中的至少一个的最后功能密码子之后的第一碱基与FRT前的最后碱基之间的多核苷酸序列插入，缺失和/或置换的2μm家族质粒 位于与所述基因相邻的反向重复位点。
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公开(公告)号：US20080261861A1
公开(公告)日：2008-10-23
申请号：US10584486
申请日：2004-12-23
CPC分类号： C12N15/81
摘要： The present invention provides a 2 μm-family plasmid comprising a polynucleotide sequence insertion, deletion and/or substitution between the first base after the last functional codon of at least one of either a REP2 gene or an FLP gene and the last base before the FRT site in an inverted repeat adjacent to said gene.
摘要翻译： 本发明提供了包含在REP2基因或FLP基因中的至少一个的最后一个功能密码子之后的第一碱基与FRT前的最后碱基之间的多核苷酸序列插入，缺失和/或置换的2个家族质粒 位于与所述基因相邻的反向重复位点。
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公开(公告)号：US20080193977A1
公开(公告)日：2008-08-14
申请号：US11722539
申请日：2005-12-23
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
摘要翻译： 本发明提供了一种生产所需蛋白质（例如所需异源蛋白质）的方法，包括：（a）提供宿主细胞，其包含编码包含第一伴侣蛋白序列的蛋白质的第一重组基因，编码第二分子伴侣蛋白的第二重组基因 包含第二伴侣蛋白和第三基因的序列的蛋白质，例如编码所需蛋白质（例如所需异源蛋白质）的第三重组基因，其中第一和第二伴侣不同; 和（b）在培养基中培养宿主细胞以获得第一，第二和第三基因的表达。
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公开(公告)号：US09057061B2
公开(公告)日：2015-06-16
申请号：US11722539
申请日：2005-12-23
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
摘要翻译： 本发明提供了一种生产所需蛋白质（例如所需异源蛋白质）的方法，包括：（a）提供宿主细胞，其包含编码包含第一伴侣蛋白序列的蛋白质的第一重组基因，编码第二分子伴侣蛋白的第二重组基因 包含第二伴侣蛋白和第三基因的序列的蛋白质，例如编码所需蛋白质（例如所需异源蛋白质）的第三重组基因，其中第一和第二伴侣不同; 和（b）在培养基中培养宿主细胞以获得第一，第二和第三基因的表达。
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公开(公告)号：US20120231503A1
公开(公告)日：2012-09-13
申请号：US13474317
申请日：2012-05-17
IPC分类号： C12P21/00 , C12P21/08 , C12N9/72 , C12N1/19 , C12N9/50 , C12N9/04 , C12N9/18 , C12N15/63 , C12N9/74
CPC分类号： C12N15/80
摘要： The present disclosure relates to a method for producing heterologous protein including: (c) providing a host cell comprising a 2 μm-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (d) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (e) purifying the thus expressed heterologous protein from the cultured host cell or the culture medium.
摘要翻译： 本公开内容涉及用于产生异源蛋白质的方法，包括：（c）提供包含2μm家族质粒的宿主细胞，所述质粒包含编码包含伴侣蛋白序列的蛋白质的基因和编码异源蛋白质的基因 ; （d）在允许表达编码伴侣蛋白的基因和编码异源蛋白质的基因的条件下，在培养基中培养宿主细胞; （e）从培养的宿主细胞或培养基纯化如此表达的异源蛋白质。
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公开(公告)号：US20120231505A1
公开(公告)日：2012-09-13
申请号：US13474313
申请日：2012-05-17
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
摘要翻译： 本发明提供了一种生产所需蛋白质（例如所需异源蛋白质）的方法，包括：（a）提供宿主细胞，其包含编码包含第一伴侣蛋白序列的蛋白质的第一重组基因，编码第二分子伴侣蛋白的第二重组基因 包含第二伴侣蛋白和第三基因的序列的蛋白质，例如编码所需蛋白质（例如所需异源蛋白质）的第三重组基因，其中第一和第二伴侣不同; 和（b）在培养基中培养宿主细胞以获得第一，第二和第三基因的表达。
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公开(公告)号：US20110313133A1
公开(公告)日：2011-12-22
申请号：US13201123
申请日：2010-02-11
申请人： Christopher John Arthur Finnis , Joanna Mary Hay , Esben Peter Friis , Jason Cameron , Darrell Sleep
发明人： Christopher John Arthur Finnis , Joanna Mary Hay , Esben Peter Friis , Jason Cameron , Darrell Sleep
CPC分类号： C07K14/765 , C07K14/76 , C07K2319/00 , G06F19/12
摘要： Based on the three-dimensional structure of albumin, the inventors have designed variant polypeptides (muteins) which have one or more cysteine residues with a free thiol group (hereinafter referred to as “thio-albumin”). The variant polypeptide may be conjugated through the sulphur atom of the cysteine residue to a conjugation partner such as a bioactive compound.
摘要翻译： 基于白蛋白的三维结构，本发明人设计了具有一个或多个具有游离巯基的半胱氨酸残基的变体多肽（突变蛋白）（以下称为“硫代白蛋白”））。 变体多肽可以通过半胱氨酸残基的硫原子缀合到缀合配偶体例如生物活性化合物。
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公开(公告)号：US20110020865A1
公开(公告)日：2011-01-27
申请号：US11993335
申请日：2006-06-22
CPC分类号： C12N15/81 , C07K14/395 , C12P21/00
摘要： The present invention provides a host cell suitable for enhanced production of a protein product of choice characterised in that the host cell is genetically modified to cause over-expression of two or more helper proteins selected from a DnaJ-like protein (such as JEM1), an Hsp70 family protein (such as LHS1) and SIL1 wherein at least one of the over-expressed two or more helper proteins is selected from JEM1, LHS1 and SIL1, and wherein the DnaJ-like protein is not SCJ1.
摘要翻译： 本发明提供了适合于增强产生所选蛋白质产物的宿主细胞，其特征在于宿主细胞被遗传修饰以引起选自DnaJ样蛋白（例如JEM1）的两种或更多种辅助蛋白的过表达， Hsp70家族蛋白（例如LHS1）和SIL1，其中至少一种过表达的两种或更多种辅助蛋白选自JEM1，LHS1和SIL1，并且其中DnaJ样蛋白不是SCJ1。
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