-
1.发明授权公开(公告)号：US06984499B2
公开(公告)日：2006-01-10
申请号：US08942369
申请日：1997-10-02
申请人： Chun-Ming Chen , Charles R. Carpenter , Haoyi Gu , Ali Naqui
发明人： Chun-Ming Chen , Charles R. Carpenter , Haoyi Gu , Ali Naqui
摘要： The present invention relates to a method of detecting the presence of target microorganisms in a biological sample and of simultaneously determining the susceptibility of the microorganisms to antimicrobial agents. The target microbial organisms may be urinary pathogens. The methods include the steps of providing a multicompartment assay device with at least one compartment containing a medium capable of sustaining the growth of total viable microorganisms, at least one compartment containing a medium capable of sustaining the growth of target microorganisms, and at least one compartment containing an antimicrobial susceptibility interpretation medium. A biological sample is placed in each compartment and the presence and antimicrobial susceptibility of the target microorganisms which may be present is determined by analyzing which compartments exhibit microbial growth.
摘要翻译： 本发明涉及一种检测生物样品中目标微生物的存在并同时测定微生物对抗微生物剂的敏感性的方法。 目标微生物可能是尿病原体。 所述方法包括以下步骤：提供具有至少一个隔室的步骤，所述隔室包含能够维持总存活微生物生长的培养基，至少一个含有能够维持靶微生物生长的培养基的隔室，以及至少一个室 含有抗微生物敏感性解释培养基。 将生物样品置于每个隔室中，通过分析哪些隔室呈现微生物生长来确定可能存在的目标微生物的存在和抗微生物敏感性。
-
2.发明授权Methods and devices for the detection of pathogenic microorganisms and their antimicrobial susceptibility 有权用于检测病原微生物及其抗菌药物敏感性的方法和装置公开(公告)号：US07384778B2
公开(公告)日：2008-06-10
申请号：US10340569
申请日：2003-01-09
申请人： Chun-Ming Chen , Charles Carpenter , Haoyi Gu , Ali Naqui , Gary Madsen , Thomas O'Connor, Jr. , Katherine Jean Esty
发明人： Chun-Ming Chen , Charles Carpenter , Haoyi Gu , Ali Naqui , Gary Madsen , Thomas O'Connor, Jr. , Katherine Jean Esty
CPC分类号： B01L3/50853 , B01L2300/045 , B01L2300/0809 , C12Q1/04 , C12Q1/18
摘要： The present invention provides devices and methods for determining the presence or absence of pathogens in a biological sample and for the concurrent determination of the antimicrobial susceptibility of pathogens present. The devices and methods of the present invention are able to make the determinations without pre-selection of target bacteria. Instead, all organisms from the sample are applied to the device. In wells where one or more antimicrobial agents are present only pathogens resistant to the antimicrobial agent grow. This allows the user to determine the presence of target pathogens and concurrently determine the resistance patterns of the organisms without the need to subculture the organisms. The present invention also provides methods of performing a business service of performing the determinations described above, and kits containing the devices and descriptions of the methods.
摘要翻译： 本发明提供用于确定生物样品中病原体的存在或不存在以及同时测定存在的病原体的抗微生物易感性的装置和方法。 本发明的装置和方法能够在不预先选择目标细菌的情况下进行测定。 相反，将来自样品的所有生物体施加到装置上。 在存在一种或多种抗微生物剂的孔中，只有抗微生物剂抗性的病原体生长。 这允许用户确定目标病原体的存在并且同时确定生物体的抗性模式，而不需要传代培养生物体。 本发明还提供了执行上述确定的业务服务的方法，以及包含所述设备的方法和所述方法的描述的套件的方法。
-
公开(公告)号：US06060266A
公开(公告)日：2000-05-09
申请号：US43960
申请日：1998-09-28
申请人： Ali Naqui , Erwin F. Workman, Jr. , Haoyi Gu , Mark W. Pierson
发明人： Ali Naqui , Erwin F. Workman, Jr. , Haoyi Gu , Mark W. Pierson
CPC分类号： C12M23/08 , C12M23/38 , C12M23/54 , C12M41/14 , F24J1/00 , Y10S435/848 , Y10S435/849 , Y10S435/975
摘要： A self-contained incubator for growth of microorganism kit and methods for use of such a kit are provided. The kit and methods may detect the presence of microorganisms and may utilie a microorganism growth and indicator medium provided in a sample container along with a heat source, preferably generating heat through chemical means, and optionally heat shields, allowing for on-site testing of a microorganism present in a sample. The sample container may also include a removable vessel cap that includes a barrier separating the sample from a material capable of disinfecting the sample, thereby preventing contact of the sample and the material for a desired time period. The vessel cap may also be used independently in other applications.
摘要翻译： PCT No.PCT / US96 / 15525 Sec。 371日期：1998年9月28日 102（e）1998年9月28日PCT PCT 1996年9月27日PCT公布。 第WO97 / 12029号公报 日期1997年4月3日提供了用于生长微生物试剂盒的独立培养箱和使用这种试剂盒的方法。 试剂盒和方法可以检测微生物的存在，并且可以利用提供在样品容器中的微生物生长和指示剂介质以及热源，优选通过化学方法产生热量，并且可选地加热屏蔽，允许现场测试 存在于样品中的微生物。 样品容器还可以包括可移除的容器盖，其包括将样品与能够对样品进行消毒的材料隔离的屏障，从而防止样品和材料在期望的时间段内接触。 容器盖也可以在其它应用中独立使用。
-
4.发明授权
公开(公告)号：US5854011A
公开(公告)日：1998-12-29
申请号：US769512
申请日：1996-12-19
申请人： Chun-Ming Chen , Haoyi Gu
发明人： Chun-Ming Chen , Haoyi Gu
CPC分类号： C12Q1/37 , C12Q1/045 , C12Q2334/22 , C12Q2337/22
摘要： A composition and method for detecting the presence or amount of yeasts and molds in a test sample is presented. The composition contains a substrate and an inhibitor for an aminopeptidase. The substrate has a signal moiety capable of providing a detectable signal when cleaved by an aminopeptidase in yeasts or molds. The aminopeptidase inhibitor serves to reduce the endogenous aminopeptidase activity in the test sample. The method to detect yeasts or molds in a sample includes inoculating a test sample with the disclosed composition, incubating the sample and observing any detectable signal that indicates the presence of yeasts or molds.
摘要翻译： 提出了一种用于检测试样中酵母和霉菌的存在或数量的组合物和方法。 该组合物含有底物和用于氨基肽酶的抑制剂。 底物具有能够在酵母或霉菌中被氨肽酶切割时能够提供可检测信号的信号部分。 氨基肽酶抑制剂用于减少测试样品中的内源氨基肽酶活性。 检测样品中的酵母或霉菌的方法包括用所公开的组合物接种测试样品，培养样品并观察任何可检测的信号，该信号指示存在酵母或霉菌。
-
5.发明授权
公开(公告)号：US6022698A
公开(公告)日：2000-02-08
申请号：US156215
申请日：1998-09-18
申请人： Chun-Ming Chen , Haoyi Gu
发明人： Chun-Ming Chen , Haoyi Gu
CPC分类号： C12Q1/37 , C12Q1/045 , C12Q2334/22 , C12Q2337/22
摘要： A composition for use in a medium for detecting yeasts and molds in a test sample, the composition comprises a substrate for an aminopeptidase; and a signal moiety linked to the substrate, the signal moiety capable of providing a detectable signal when cleaved by aminopeptidase in yeasts or molds. Optionally, an aminopeptidase inhibitor is added to the composition, to reduce endogenous enzymatic activity in samples. A method for use of the composition to detect the presence or amount of yeasts and/or molds in a test sample comprises inoculating a medium comprising the composition of claim 1 with said test sample; incubating the inoculated medium under conditions suitable for the growth of yeasts or molds; and, observing any detectable signal, whereby the presence of a detectable signal indicates the presence of yeasts or molds in the test sample.
摘要翻译： 一种用于在测试样品中检测酵母和霉菌的培养基中的组合物，该组合物包含氨基肽酶的底物; 和与底物连接的信号部分，该信号部分能够在酵母或霉菌中被氨基肽酶切割时提供可检测的信号。 任选地，将氨基肽酶抑制剂加入到组合物中，以降低样品中的内源性酶活性。 使用组合物来检测测试样品中酵母和/或霉菌的存在或数量的方法包括将包含权利要求1的组合物的培养基与所述测试样品接种; 在适合于酵母或霉菌生长的条件下孵育接种的培养基; 并且观察任何可检测的信号，由此可检测信号的存在表明在测试样品中存在酵母或霉菌。
-
公开(公告)号：US07582472B2
公开(公告)日：2009-09-01
申请号：US10899217
申请日：2004-07-26
CPC分类号： B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L2300/0803 , B01L2300/0864 , B01L2400/0406
摘要： There is provided a device for partitioning a liquefied sample into discrete volumes. The device includes a bottom member; a top member disposed adjacent the bottom member; and at least one channel member disposed between the top and bottom members. The at least one channel member is at least partially defined by the top and bottom members and has first and second end portions. The first end portion of the at least one channel has an opening to receive liquid and the second end portion of the at least one channel has a reaction compartment and a vent opening. Accordingly, when the liquefied sample is introduced to the first end portion, capillary action assists in causing the liquefied sample to travel from the first end portion to the second end portion and at least a portion of the liquefied sample is caused to remain in the reaction compartment.
摘要翻译： 提供了用于将液化样品分成离散体积的装置。 该装置包括底部构件; 邻近所述底部构件设置的顶部构件; 以及设置在顶部和底部构件之间的至少一个通道构件。 至少一个通道构件至少部分地由顶部构件和底部构件限定，并具有第一和第二端部。 所述至少一个通道的第一端部具有用于接收液体的开口，并且所述至少一个通道的第二端部具有反应室和通风口。 因此，当液化样品被引入到第一端部时，毛细管作用有助于使液化样品从第一端部移动到第二端部，并且使至少一部分液化样品保持在反应中 隔间
-
公开(公告)号：US20050048597A1
公开(公告)日：2005-03-03
申请号：US10899217
申请日：2004-07-26
申请人： Kenneth Smith , Haoyi Gu , Scott Wagner , Scott Clark , Chun-Ming Chen
发明人： Kenneth Smith , Haoyi Gu , Scott Wagner , Scott Clark , Chun-Ming Chen
CPC分类号： B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L2300/0803 , B01L2300/0864 , B01L2400/0406
摘要： There is provided a device for partitioning a liquefied sample into discrete volumes. The device includes a bottom member; a top member disposed adjacent the bottom member; and at least one channel member disposed between the top and bottom members. The at least one channel member is at least partially defined by the top and bottom members and has first and second end portions. The first end portion of the at least one channel has an opening to receive liquid and the second end portion of the at least one channel has a reaction compartment and a vent opening. Accordingly, when the liquefied sample is introduced to the first end portion, capillary action assists in causing the liquefied sample to travel from the first end portion to the second end portion and at least a portion of the liquefied sample is caused to remain in the reaction compartment.
摘要翻译： 提供了用于将液化样品分成离散体积的装置。 该装置包括底部构件; 邻近所述底部构件设置的顶部构件; 以及设置在顶部和底部构件之间的至少一个通道构件。 至少一个通道构件至少部分地由顶部构件和底部构件限定，并具有第一和第二端部。 所述至少一个通道的第一端部具有用于接收液体的开口，并且所述至少一个通道的第二端部具有反应室和通风口。 因此，当液化样品被引入到第一端部时，毛细管作用有助于使液化样品从第一端部移动到第二端部，并且使至少一部分液化样品保持在反应中 隔间
-
公开(公告)号：US5620865A
公开(公告)日：1997-04-15
申请号：US335149
申请日：1994-11-04
申请人： Chun-Ming Chen , Haoyi Gu
发明人： Chun-Ming Chen , Haoyi Gu
IPC分类号： C12Q1/10 , C12Q1/04 , C12Q1/54 , G01N33/569
CPC分类号： C12Q1/10 , Y10S435/885
摘要： Medium for growing Enterococci so that detection may be obtained within 24 hours.
摘要翻译： 培养肠球菌，以便可以在24小时内获得检测。
-
公开(公告)号：US07534600B2
公开(公告)日：2009-05-19
申请号：US11434287
申请日：2006-05-15
申请人： Haoyi Gu
发明人： Haoyi Gu
摘要： The invention provides devices and methods for rapid quantification of microorganisms and cells. In one embodiment, the devices comprise a container having an upper compartment and a lower compartment, wherein the upper compartment is connected to a passage through which a fluid sample can flow and enter the upper compartment, and wherein the lower compartment is connected to a passage through which a fluid sample can flow and exit the lower compartment; a mounting structure upon which a filter can be positioned; a filter, wherein the filter separates the upper compartment from the lower compartment, wherein the filter comprises a chromogen; a first template covering the filter, wherein the first template comprises a predetermined number of holes through which a liquid sample can flow through to discrete locations on the filter; and an absorbent pad under the filter and in contact with the filter.
摘要翻译： 本发明提供用于快速定量微生物和细胞的装置和方法。 在一个实施例中，装置包括具有上隔室和下隔室的容器，其中上隔室连接到流体样品可以流过并进入上隔室的通道，并且其中下隔室连接到通道 流体样品可以流过并离开下隔室; 安装结构，过滤器可以定位在其上; 过滤器，其中过滤器将上隔室与下隔室分离，其中过滤器包括色原体; 覆盖所述过滤器的第一模板，其中所述第一模板包括预定数量的孔，液体样本可以穿过所述孔流过所述过滤器上的离散位置; 以及在过滤器下方并与过滤器接触的吸收垫。
-
-
-
-
-
-
-
-
-
搜索结果
10 条记录 (耗时 0.027 秒)
