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    Method to measure a t cell response and its uses to qualify antigen-presenting cells
    3.
    发明申请
    Method to measure a t cell response and its uses to qualify antigen-presenting cells 审中-公开
    测量t细胞应答的方法及其用于鉴定抗原呈递细胞的方法

    公开(公告)号：US20060160153A1

    公开(公告)日：2006-07-20

    申请号：US10537500

    申请日：2003-12-02

    申请人： Jean-Pierre Abastado ,  Nadege Bercovici ,  Marc Ernstoff ,  Alice Givan ,  Alessandra Nardin ,  Margarita Magguilli born Salcedo ,  Paul Wallace

    发明人： Jean-Pierre Abastado ,  Nadege Bercovici ,  Marc Ernstoff ,  Alice Givan ,  Alessandra Nardin ,  Margarita Magguilli born Salcedo ,  Paul Wallace

    IPC分类号： G01N33/567

    CPC分类号： G01N33/5094 ,  G01N33/505 ,  G01N33/56972

    摘要： A method to characterize a T-cell response of a final population of T-lymphocytes resulting from the co-incubation of an initial population of T lymphocytes with a composition of antigen-presenting cells (APCs), said method comprising the steps of a) simultaneous measuring on a single cell basis at least two parameters: (i) proliferation of T lymphocytes and (ii) presence of a T cell antigen receptor on the surface of T lymphocytes and/or presence of at least one biological molecule produced by T lymphocytes, and the attribution of a positive or a negative value to each of these parameters, and b) classification of the final T-lymphocytes population into 2n different subsets of T lymphocytes, n being the number of parameters, each subset being characterized by a positive or a negative value respectively to each parameter and the determination of the proportion of T lymphocytes present in each subset with respect to the number of T lymphocytes in the final population, with said proportion being characteristic of the T-cell response. Use of the method for batch release assay and potency assay.

    摘要翻译： 表征由初始T淋巴细胞群与抗原呈递细胞（APC）的组合共孵育的最终T淋巴细胞群的T细胞应答的方法，所述方法包括以下步骤：a） 在单细胞基础上同时测量至少两个参数：（i）T淋巴细胞的增殖和（ii）T淋巴细胞表面上存在T细胞抗原受体和/或由T淋巴细胞产生的至少一种生物分子的存在 ，以及对这些参数中的每一个的正值或负值的归属，以及b）将最终T淋巴细胞群分类为T淋巴细胞的2个不同子集，n是参数的数目 每个子集分别以每个参数的正值或负值表示，并确定每个子集中存在的T淋巴细胞相对于最终群体中T淋巴细胞数目的比例，其中sa id比例是T细胞应答的特征。 使用批次释放测定法和效力测定法。

    Altered major histocompatibility complex (MHC) determinant and methods of using the determinant
    7.
    发明授权
    Altered major histocompatibility complex (MHC) determinant and methods of using the determinant 失效
    改变主要组织相容性复合体（MHC）的决定因素和使用决定簇的方法

    公开(公告)号：US6153408A

    公开(公告)日：2000-11-28

    申请号：US370476

    申请日：1995-01-09

    申请人： Jean-Pierre Abastado ,  Estelle Mottez ,  Philippe Kourilsky ,  Armanda Casrouge ,  David Ojcius ,  Yu-Chun Lone

    发明人： Jean-Pierre Abastado ,  Estelle Mottez ,  Philippe Kourilsky ,  Armanda Casrouge ,  David Ojcius ,  Yu-Chun Lone

    IPC分类号： A61K38/00 ,  C07K14/74 ,  C07K19/00 ,  A61K39/385 ,  C12N15/62 ,  C12N15/85

    CPC分类号： C07K14/70539 ,  A61K38/00 ,  C07K2319/00

    摘要： An altered MHC class I determinant comprises .alpha..sub.1, .alpha..sub.2, .alpha..sub.3, .beta..sub.2 -microglobulin (.beta..sub.2 m) polypeptide domains encoded by a mammalian MHC class I locus in which the .alpha..sub.3 domain is covalently linked to the .beta..sub.2 M domain. An altered MHC class II determinant comprises .alpha..sub.1, .alpha..sub.2, .beta..sub.1, and .beta..sub.2 polypeptide domains encoded by a mammalian MHC class II locus, in which the domains are covalently linked to form a polypeptide comprising the .beta..sub.2 -.alpha..sub.2 -.alpha..sub.1 -.beta..sub.1 domains in sequence. The altered MHC class I and class II determinants can be associated with an antigen to elicit an immune response. The invention can be used in the immunization or treatment of diseases such as AIDS, multiple sclerosis, lupus erythematosus, toxic shock or snake bite.

    摘要翻译： 改变的MHC I类决定簇包含由哺乳动物MHC I类基因座编码的α1，α2，α3，β2-微球蛋白（β2m）多肽结构域，其中α3结构域与β2M结构域共价连接。 改变的MHC II类决定簇包含由哺乳动物MHC II类基因座编码的α1，α2，β1和β2多肽结构域，其中所述结构域共价连接以形成包含β2-α2- 1-β1结构域序列。 改变的MHC I类和II类决定簇可以与抗原相关以引发免疫应答。 本发明可用于免疫或治疗AIDS，多发性硬化症，红斑狼疮，中毒性休克或蛇咬伤等疾病。

    Altered major histocompatibility complex (MHC) determinant and method of using the determinant
    8.
    发明授权
    Altered major histocompatibility complex (MHC) determinant and method of using the determinant 失效
    改变主要组织相容性复合体（MHC）的决定因素和使用决定簇的方法

    公开(公告)号：US5976551A

    公开(公告)日：1999-11-02

    申请号：US484905

    申请日：1995-06-07

    申请人： Estelle Mottez ,  Jean-Pierre Abastado ,  Philippe Kourilsky

    发明人： Estelle Mottez ,  Jean-Pierre Abastado ,  Philippe Kourilsky

    IPC分类号： A61K38/00 ,  A61P37/02 ,  C07K14/74 ,  A61K39/385 ,  C07K16/74

    CPC分类号： C07K14/70539 ,  A61K38/00 ,  C07K2319/00

    摘要： An altered MHC class II determinant comprises .alpha..sub.1, .alpha..sub.2, .beta..sub.1, .beta..sub.2, domains of a mammalian MHC class II locus in which the domains are covalently linked to form a construct comprising the .beta..sub.2 -.alpha..sub.2 -.alpha..sub.1 .beta..sub.1 domains in sequence. The altered class II determinants can be associated with an antigen to elicit an immune response. In addition, the altered determinants may be used to prepare antibodies. The antibodies so produced have various diagnostic and therapeutic uses.

    摘要翻译： 改变的MHC II类决定簇包含α1，α2，β1，β2，哺乳动物MHC II类基因座的结构域，其中所述结构域共价连接以形成包含β2-α2-α1β1 域序列。 改变的II类决定簇可以与抗原相关以引发免疫应答。 此外，改变的决定簇可用于制备抗体。 所产生的抗体具有各种诊断和治疗用途。