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    Genomic DNA fragment of Streptococcus pneumontiae, hybridization probe, amplification primer, reagent and method for the detection of Streptococcus pneumoniae
    2.
    发明授权
    Genomic DNA fragment of Streptococcus pneumontiae, hybridization probe, amplification primer, reagent and method for the detection of Streptococcus pneumoniae 失效
    肺炎链球菌基因组DNA片段，杂交探针，扩增引物，肺炎链球菌检测试剂及方法

    公开(公告)号：US5776691A

    公开(公告)日：1998-07-07

    申请号：US419765

    申请日：1995-04-10

    申请人： Jean-Pierre Claverys ,  Claude Mabilat

    发明人： Jean-Pierre Claverys ,  Claude Mabilat

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689

    摘要： The invention relates to a fragment of the genomic DNA of Streptococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Screptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.

    摘要翻译： 本发明涉及肺炎链球菌的基因组DNA的片段，能够与肺炎链球菌的基因组DNA特异性杂交的探针，用于扩增，聚合的特异性引物，基因组DNA，试剂和方法 与探针一起使用，以及任选的底漆，用于特异性检测生物样品中的肺炎链球菌。 本发明的探针是与肺炎链球菌的基因组DNA的共有序列的至少一部分具有至少70％同源性的核苷酸序列，该共有序列选自核苷酸序列SEQ ID NO：2，SEQ ID NO： ，SEQ ID NO 4，以及它们各自的互补序列。

    DNA fragments, probes and amplification primers of the 65 kD antigen of mycobacteria
    3.
    发明授权
    DNA fragments, probes and amplification primers of the 65 kD antigen of mycobacteria 失效
    分子细菌65 kD抗原的DNA片段，探针和扩增引物

    公开(公告)号：US5589585A

    公开(公告)日：1996-12-31

    申请号：US105168

    申请日：1993-08-12

    申请人： Claude Mabilat ,  Jean-Claude Pechere

    发明人： Claude Mabilat ,  Jean-Claude Pechere

    IPC分类号： A61K39/00 ,  C07K14/35 ,  C12N15/31 ,  C12P21/08 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  C07K14/35 ,  A61K39/00

    摘要： Nucleotide fragment of DNA, whose nucleotide sequence is included in the gene of the species of the genus Mycobacterium, coding for the 65-kD mycobacterial antigen, containing regions which are homologous in practically all species of the genus Mycobacterium, and at least one species-specific variable region, characterized in that said fragment is chosen from fragments whose nucleotide sequences possess at least 70% homology, and preferably at least 85% homology, with a predetermined sequence or its complementary sequence, said predetermined sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinurn.

    摘要翻译： DNA的核苷酸片段，其核苷酸序列包括在分枝杆菌属的种类的基因中，编码65-kD分枝杆菌抗原，其含有几乎所有分枝杆菌属物种中同源的区域，以及至少一种物种 - 特征在于所述片段选自其核苷酸序列与预定序列或其互补序列具有至少70％同源性，优选至少85％同源性的片段，所述预定序列从核苷酸438开始并以 编码所有分枝杆菌种类的所述抗原的所述基因的核苷酸751除了结核分枝杆菌，牛分枝杆菌BCG，鸟分枝杆菌，副结核分枝杆菌，马铃薯，马尔莫森，麻疯树，马萨诸塞州 和M. marinurn。

    DNA fragments of mycobacteria, amplification primers hybridization probes, reagents and method for the detection of mycobacteria
    4.
    发明授权
    DNA fragments of mycobacteria, amplification primers hybridization probes, reagents and method for the detection of mycobacteria 失效
    分枝杆菌的DNA片段，扩增引物杂交探针，试剂和检测分枝杆菌的方法

    公开(公告)号：US5849901A

    公开(公告)日：1998-12-15

    申请号：US698948

    申请日：1996-08-16

    申请人： Claude Mabilat ,  Jean-Claude Pechere

    发明人： Claude Mabilat ,  Jean-Claude Pechere

    IPC分类号： A61K39/00 ,  C07K14/35 ,  C12N15/31 ,  C12P21/08 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  C07K14/35 ,  A61K39/00

    摘要： Nucleotide fragment of DNA, whose nucleotide sequence is included in the gene of the species of the genus Mycobacterium, coding for the 65-kD mycobacterial antigen, containing regions which are homologous in practically all species of the genus Mycobacterium, and at least one species-specific variable region, characterized in that said fragment is chosen from fragments whose nucleotide sequences possess at least 70% homology, and preferably at least 85% homology, with a predetermined sequence or its complementary sequence, said predetermined sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinum.

    摘要翻译： DNA的核苷酸片段，其核苷酸序列包括在分枝杆菌属的种类的基因中，编码65-kD分枝杆菌抗原，其含有几乎所有分枝杆菌属物种中同源的区域，以及至少一种物种 - 特征在于所述片段选自其核苷酸序列与预定序列或其互补序列具有至少70％同源性，优选至少85％同源性的片段，所述预定序列从核苷酸438开始并以 编码所有分枝杆菌种类的所述抗原的所述基因的核苷酸751除了结核分枝杆菌，牛分枝杆菌BCG，鸟分枝杆菌，副结核分枝杆菌，马铃薯，马尔莫森，麻疯树，马萨诸塞州 和M. marinum。

    Device for the capture of target molecules, and capturing process using the device
    5.
    发明授权
    Device for the capture of target molecules, and capturing process using the device 失效
    用于捕获目标分子的装置，以及使用该装置的捕获过程

    公开(公告)号：US5723344A

    公开(公告)日：1998-03-03

    申请号：US264996

    申请日：1994-06-24

    申请人： Claude Mabilat ,  Philippe Cros ,  Bernard Mandrand ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    发明人： Claude Mabilat ,  Philippe Cros ,  Bernard Mandrand ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC分类号： G01N33/543 ,  C12Q1/68 ,  G01N33/544

    CPC分类号： G01N33/54353 ,  G01N33/54366 ,  Y10S435/975 ,  Y10S436/807

    摘要： Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption. When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.

    摘要翻译： 用于捕获靶分子以用于检测和/或测定靶分子的装置，包括其上固定有配体的固体支持体，所述配体以由聚合物与多个共价键共价偶联得到的缀合物形式提供 的配体分子。 聚合物是N-乙烯基吡咯烷酮共聚物，并且通过吸附将缀合物固定在固体载体上。 当配体能够与目标物形成复合物时，该装置对于给定的靶标是特异性的。 此外，当该装置另外包含能够与配体一起形成络合物的双功能试剂，另一方面，另外，与靶标配位的配体固定的载体构成通用捕获系统。

    Nucleotide fragment of the 23S ribosomal RNA of mycobacteria, derived probes and primers, reagent and detection method
    6.
    发明授权
    Nucleotide fragment of the 23S ribosomal RNA of mycobacteria, derived probes and primers, reagent and detection method 失效
    分枝杆菌23S核糖体RNA的核苷酸片段，衍生探针和引物，试剂和检测方法

    公开(公告)号：US5703217A

    公开(公告)日：1997-12-30

    申请号：US403762

    申请日：1995-03-23

    申请人： Claude Mabilat ,  Richard Christen

    发明人： Claude Mabilat ,  Richard Christen

    IPC分类号： C07K14/35 ,  C12N15/31 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C07K14/35 ,  C12Q1/689

    摘要： Bacteria of the genus Mycobacterium can be detected and/or identified by methods using probes including fragments of a variable region of a 23S ribosomal RNA of a species of the genus Mycobacterium or probes including DNA fragments that are obtained by reverse transcription of the RNA or that form the RNA by transcription. Primers for the reverse transcription of a variable region of a 23S ribosomal RNA sequence of mycobacteria include nucleotide sequences of a 23S RNA of a species of the genus Mycobacterium.

    摘要翻译： PCT No.PCT / FR94 / 00929 Sec。 371日期：1995年3月23日 102（e）1995年3月23日PCT PCT 1994年7月22日PCT公布。 第WO95 / 03412号公报 日期1995年2月2日可以通过使用包括分枝杆菌属的23S核糖体RNA的可变区的片段的探针或包括通过逆转录获得的DNA片段的探针的方法来检测和/或鉴定细菌属的细菌 的RNA或通过转录形成RNA。 用于分枝杆菌的23S核糖体RNA序列的可变区的逆转录的引物包括分枝杆菌属的23S RNA的核苷酸序列。

    Detection of bacteria of genus Listeria using nucleic probe hybridization techniques
    7.
    发明授权
    Detection of bacteria of genus Listeria using nucleic probe hybridization techniques 失效
    使用核酸探针杂交技术检测利斯特氏菌属细菌

    公开(公告)号：US6090551A

    公开(公告)日：2000-07-18

    申请号：US875296

    申请日：1997-09-29

    申请人： Claude Mabilat ,  Brunehild Sallen

    发明人： Claude Mabilat ,  Brunehild Sallen

    IPC分类号： C12N15/09 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/01 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/689

    摘要： A single-stranded nucleotide fragment belonging to a variable region of the ribosomal RNA 23S of species of the genus mycobacterium. Probes and primers with sequences belonging to those of the single-stranded nucleotide fragments, a reagent and a method for identifying the mycobacterial species.

    摘要翻译： PCT No.PCT / FR96 / 00202 Sec。 371日期：1997年9月29日 102（e）1997年9月29日PCT PCT 1996年2月7日PCT公布。 出版物WO96 / 24686 日期：1996年8月15日属于分枝杆菌属的核糖体RNA 23S的可变区的单链核苷酸片段。 具有属于单链核苷酸片段的序列的探针和引物，试剂和鉴定分枝杆菌物种的方法。

    Nucleic acid isolation
    10.
    发明授权
    Nucleic acid isolation 失效
    核酸分离

    公开(公告)号：US06737235B1

    公开(公告)日：2004-05-18

    申请号：US08945731

    申请日：1997-11-10

    申请人： Philippe Cros ,  Abdelhamid Elaissari ,  Claude Mabilat ,  Christian Pichot ,  Marc Rodrigue ,  Lise Santoro

    发明人： Philippe Cros ,  Abdelhamid Elaissari ,  Claude Mabilat ,  Christian Pichot ,  Marc Rodrigue ,  Lise Santoro

    IPC分类号： C07H2104

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

    摘要： A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45° C.; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10−2 M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10−2 M is achieved.

    摘要翻译： 公开了一种从样品中分离水相核酸的方法，包括在颗粒基质上的核酸吸附步骤。 该方法包括一种吸附剂制备步骤（a），其包括由水性连续相组成的溶胶和包含通过聚合（1）第一水溶性丙烯酰胺或丙烯酰胺衍生物单体制备的官能化颗粒聚合物的分散颗粒基质相（ 2）至少一种交联剂和（3）至少一种第二水溶性阳离子和功能性单体，所述聚合物具有25-45℃的预定的较低临界溶解度温度（LCST）。 接触步骤（b），其中所述吸附剂与含有所述核酸的样品接触; 吸附步骤（c）其中，为了进行接触步骤（b），为反应介质选择至少一个参数，所述参数为不高于7的pH，不高于10 -2的离子强度 M，温度低于聚合物LCST; 分离步骤（d），其中分散相与连续相分离，任选地在已经观察到发生吸附之后; 和解吸步骤（e），其中通过增加离子强度直到达到高于10μM的离子强度，从核粒子脱离。