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公开(公告)号：US20240301486A1
公开(公告)日：2024-09-12
申请号：US18592885
申请日：2024-03-01
Applicant: Complete Genomics, Inc
Inventor： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
IPC: C12Q1/6874 , C07H21/04 , C07K1/04 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , G01N15/1404 , G01N15/1434
CPC classification number: C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , G01N15/1404 , G01N15/1434 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882
Abstract: Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
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公开(公告)号：US20180016628A1
公开(公告)日：2018-01-18
申请号：US15426930
申请日：2017-02-07
Applicant: Complete Genomics, Inc.
Inventor： Radoje Drmanac , Matthew J. Callow , Brian K. Hauser , George Yeung
CPC classification number: C12Q1/6837 , C12N15/1093 , C12Q1/682 , C12Q1/6869 , C12Q1/6874 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151 , C12Q2521/313 , C12Q2565/513 , C12Q2521/307
Abstract: The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
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公开(公告)号：US20170152554A1
公开(公告)日：2017-06-01
申请号：US15425791
申请日：2017-02-06
Applicant: Complete Genomics, Inc
Inventor： RADOJE DRMANAC , Matthew Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
Abstract: A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.
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公开(公告)号：US20240263230A1
公开(公告)日：2024-08-08
申请号：US18592843
申请日：2024-03-01
Applicant: Complete Genomics, Inc
Inventor： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
IPC: C12Q1/6874 , C07H21/04 , C07K1/04 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , G01N15/1404 , G01N15/1434
CPC classification number: C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , G01N15/1404 , G01N15/1434 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882
Abstract: Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
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公开(公告)号：US20220162694A1
公开(公告)日：2022-05-26
申请号：US17522708
申请日：2021-11-09
Applicant: Complete Genomics, Inc
Inventor： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
IPC: C12Q1/6874 , C12Q1/682 , C12Q1/6869 , C12Q1/6806 , C12Q1/6837 , C07H21/04 , C07K1/04 , G01N15/14
Abstract: Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer.
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Patent Agency Ranking
公开(公告)号：US20170175184A1
公开(公告)日：2017-06-22
申请号：US15442659
申请日：2017-02-25
Applicant: Complete Genomics, Inc.
Inventor： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
CPC classification number: C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
Abstract: The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.
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公开(公告)号：US20190316190A1
公开(公告)日：2019-10-17
申请号：US16189469
申请日：2018-11-13
Applicant: Complete Genomics, Inc.
Inventor： Radoje Drmanac , Matthew J. Callow , Brian K. Hauser , George Yeung
IPC: C12Q1/6837 , C12N15/10 , C12Q1/682 , C12Q1/6869 , C12Q1/6874
Abstract: The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
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公开(公告)号：US10351909B2
公开(公告)日：2019-07-16
申请号：US15442659
申请日：2017-02-25
Applicant: Complete Genomics, Inc.
Inventor： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
IPC: C12Q1/68 , C12Q1/6874 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C07H21/04 , C07K1/04 , G01N15/14 , C12Q1/6806
Abstract: The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.
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公开(公告)号：US09944984B2
公开(公告)日：2018-04-17
申请号：US15425791
申请日：2017-02-06
Applicant: Complete Genomics, Inc.
Inventor： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
CPC classification number: C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
Abstract: A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.
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公开(公告)号：US09650673B2
公开(公告)日：2017-05-16
申请号：US14714133
申请日：2015-05-15
Applicant: Complete Genomics, Inc.
Inventor： Radoje Drmanac , Matthew J. Callow , Snezana Drmanac , Brian K. Hauser , George Yeung
CPC classification number: C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
Abstract: Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
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