公开(公告)号：US20240301486A1

公开(公告)日：2024-09-12

申请号：US18592885

申请日：2024-03-01

Applicant: Complete Genomics, Inc

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/6874 ,  C07H21/04 ,  C07K1/04 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N15/1434

CPC classification number: C12Q1/6874 ,  C07H21/04 ,  C07K1/047 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N15/1434 ,  C12Q2525/151 ,  C12Q2525/313 ,  C12Q2531/125 ,  C12Q2565/513 ,  Y10S977/778 ,  Y10S977/789 ,  Y10S977/792 ,  Y10S977/88 ,  Y10S977/882

Abstract: Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.

公开(公告)号：US20180223358A1

公开(公告)日：2018-08-09

申请号：US15862566

申请日：2018-01-04

Applicant: Complete Genomics, Inc. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang ,  Quan Ding

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  A61K47/00 ,  C07K16/2851 ,  C07K16/44 ,  C07K2317/73 ,  C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2525/186

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US20140213461A1

公开(公告)日：2014-07-31

申请号：US13965166

申请日：2013-08-12

Applicant: Complete Genomics, Inc.

Inventor： Radoje Drmanac ,  Matthew J. Callow

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6855 ,  C12Q2531/125 ,  C12Q2521/313 ,  C12Q2521/125 ,  C12Q2537/163 ,  C12Q2525/191 ,  C12Q2521/531 ,  C12Q2521/501 ,  C12Q2525/307 ,  C12Q2525/151

Abstract: The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.

Abstract translation: 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

公开(公告)号：US20240263230A1

公开(公告)日：2024-08-08

申请号：US18592843

申请日：2024-03-01

Applicant: Complete Genomics, Inc

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/6874 ,  C07H21/04 ,  C07K1/04 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N15/1434

CPC classification number: C12Q1/6874 ,  C07H21/04 ,  C07K1/047 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N15/1434 ,  C12Q2525/151 ,  C12Q2525/313 ,  C12Q2531/125 ,  C12Q2565/513 ,  Y10S977/778 ,  Y10S977/789 ,  Y10S977/792 ,  Y10S977/88 ,  Y10S977/882

Abstract: Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.

公开(公告)号：US20220162694A1

公开(公告)日：2022-05-26

申请号：US17522708

申请日：2021-11-09

Applicant: Complete Genomics, Inc

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/6874 ,  C12Q1/682 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6837 ,  C07H21/04 ,  C07K1/04 ,  G01N15/14

Abstract: Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer.

公开(公告)号：US20180016628A1

公开(公告)日：2018-01-18

申请号：US15426930

申请日：2017-02-07

Applicant: Complete Genomics, Inc.

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6837 ,  C12N15/1093 ,  C12Q1/682 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/125 ,  C12Q2525/313 ,  C12Q2525/151 ,  C12Q2521/313 ,  C12Q2565/513 ,  C12Q2521/307

Abstract: The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.

公开(公告)号：US20160194686A1

公开(公告)日：2016-07-07

申请号：US14961753

申请日：2015-12-07

Applicant: Complete Genomics, Inc.

Inventor： Radoje T. Drmanac ,  Matthew J. Callow

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2539/103 ,  C12Q2531/119

Abstract: The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.

Abstract translation: 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是多核苷酸阵列，其中多核苷酸经历了多轮扩增，以增加与单个多核苷酸分子相关的信号强度。

公开(公告)号：US20190316190A1

公开(公告)日：2019-10-17

申请号：US16189469

申请日：2018-11-13

Applicant: Complete Genomics, Inc.

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/6837 ,  C12N15/10 ,  C12Q1/682 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are “self-assembled” in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.

公开(公告)号：US10351909B2

公开(公告)日：2019-07-16

申请号：US15442659

申请日：2017-02-25

Applicant: Complete Genomics, Inc.

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  C07H21/04 ,  C07K1/04 ,  G01N15/14 ,  C12Q1/6806

Abstract: The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.

公开(公告)号：US09944984B2

公开(公告)日：2018-04-17

申请号：US15425791

申请日：2017-02-06

Applicant: Complete Genomics, Inc.

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/68 ,  C07H21/04 ,  C07K1/04 ,  G01N15/14

CPC classification number: C12Q1/6874 ,  C07H21/04 ,  C07K1/047 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2525/151 ,  C12Q2525/313 ,  C12Q2531/125 ,  C12Q2565/513 ,  G01N15/1404 ,  G01N15/1434 ,  Y10S977/778 ,  Y10S977/789 ,  Y10S977/792 ,  Y10S977/88 ,  Y10S977/882 ,  C12Q2521/307 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.