公开(公告)号：US20150086424A1

公开(公告)日：2015-03-26

申请号：US14479283

申请日：2014-09-06

Applicant: CyVek, Inc.

Inventor： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood ,  Jane M. Tu

IPC: G01N33/543 ,  B01L3/00

CPC classification number: B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0832 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2300/168 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/086 ,  G01N21/05 ,  G01N21/645 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2035/00158 ,  Y10T29/494

Abstract: A microfluidic assay device that defines a micro-fluidic flow channel (44) having a flow axis, in which a series of discrete, axially-spaced apart, transparent hollow flow elements (32) are secured in fixed position, each flow element having at least one axially-extending flow passage through its interior, assay capture agent fixed to the interior surface of the elements for capture of an analyte in liquid flowing through the interior of the flow elements, the device constructed to enable light to be transmitted out of the elements for reading of fluorescence from captured analyte, wherein: the exterior axially-extending surfaces of the flow elements are free of active capture agent, while at least part of the interior surfaces carry deposits of active capture agent exposed to flow through the elements.

Abstract translation: 一种限定具有流动轴线的微流体流动通道（44）的微流体测定装置，其中一系列离散的，轴向间隔开的透明中空流动元件（32）固定在固定位置，每个流动元件具有 至少一个轴向延伸的通过其内部的流动通道，测定捕获剂固定到元件的内表面，用于捕获流过流动元件内部的液体中的分析物，该装置构造成使得光能够从 用于从捕获的分析物读取荧光的元件，其中：流动元件的外部轴向延伸的表面没有活性捕获剂，而至少部分内表面携带暴露于流过元件的活性捕获剂的沉积物。

公开(公告)号：US09500645B2

公开(公告)日：2016-11-22

申请号：US14479283

申请日：2014-09-06

Applicant: CyVek, Inc.

Inventor： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood ,  Jane M. Tu

IPC: G01N15/06 ,  B01L3/00 ,  G01N35/00 ,  G01N1/10 ,  G01N33/543 ,  G01N21/05 ,  G01N21/64 ,  G01N21/03

CPC classification number: B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0832 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2300/168 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/086 ,  G01N21/05 ,  G01N21/645 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2035/00158 ,  Y10T29/494

Abstract: A microfluidic assay device that defines a micro-fluidic flow channel (44) having a flow axis, in which a series of discrete, axially-spaced apart, transparent hollow flow elements (32) are secured in fixed position, each flow element having at least one axially-extending flow passage through its interior, assay capture agent fixed to the interior surface of the elements for capture of an analyte in liquid flowing through the interior of the flow elements, the device constructed to enable light to be transmitted out of the elements for reading of fluorescence from captured analyte, wherein:the exterior axially-extending surfaces of the flow elements are free of active capture agent, while at least part of the interior surfaces carry deposits of active capture agent exposed to flow through the elements.

Abstract translation: 流动元件的外部轴向延伸的表面没有主动捕获剂，而至少部分内表面附着有暴露于流过元件的主动捕集剂的沉积物。