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    Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
    1.
    发明授权
    Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays 有权

    公开(公告)号：US10786800B2

    公开(公告)日：2020-09-29

    申请号：US15477902

    申请日：2017-04-03

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

    IPC: G01N21/05 ,  B01J19/00 ,  G01N35/00 ,  G01N21/64 ,  B01L3/00 ,  G01N33/543 ,  G01N21/03

    Abstract: A method and system for heating and/or inspecting a portable microfluidic assay cartridge for performing an assay includes receiving the assay cartridge on a receiving region of a translatable table under automated control, heating the cartridge, during performance of the assay, with a planar radiant heater plate, the heater plate having an aperture through which an inspection axis extends, and/or inspecting the cartridge using an optical system constructed to inspect the cartridge along the inspection axis by reading a fluorescent light signal which passes through the aperture in the heater plate. In addition, the cartridge moves with movement of the translation table, and the heater plate and optical system may be stationary, and the inspection axis may be fixed.

    Microfluidic Assay Assemblies and Methods of Manufacture
    2.
    发明申请
    Microfluidic Assay Assemblies and Methods of Manufacture 审中-公开

    公开(公告)号：US20200047476A1

    公开(公告)日：2020-02-13

    申请号：US16570127

    申请日：2019-09-13

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam

    IPC: B32B37/10 ,  B32B37/00 ,  B32B37/02 ,  B01L3/00 ,  G01N21/05 ,  G01N21/64 ,  G01N33/543

    Abstract: A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.

    Portable Microfluidic Assay Devices and Methods of Manufacture and Use
    4.
    发明申请
    Portable Microfluidic Assay Devices and Methods of Manufacture and Use 有权
    便携式微流控测定装置及其制造和使用方法

    公开(公告)号：US20150083313A1

    公开(公告)日：2015-03-26

    申请号：US14479287

    申请日：2014-09-06

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam

    IPC: G01N33/53 ,  B32B37/02 ,  B32B38/00 ,  B32B37/00

    CPC classification number: B32B38/0004 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/168 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/086 ,  B32B37/0076 ,  B32B37/02 ,  G01N21/05 ,  G01N21/645 ,  G01N33/54366 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2035/00158 ,  Y10T29/494 ,  Y10T156/10 ,  Y10T156/1052

    Abstract: A method of forming a pneumatically controlled microfluidic device containing a micro-fluidic network that includes micro-elements in the form of micro-particles or micro-length hollow flow elements, and a pneumatic network that includes pneumatic micro-channels and micro-valve features enabling membrane valves to operate to control fluid conditions in the microfluidic network, including the steps (a) forming two portions, denominated fluidic layer, in which microfluidic channels are formed, and pneumatic layer, in which pneumatic micro-channels and micro-valve features are formed, each having a backing that is rigid in the plane of extent of the layers, (b) providing an intervening elastic membrane, and (c) permanently bonding both layers to opposite sides of the membrane, the permanent bonding of the membrane to the fluidic layer being effective to permanently enclose a set of inserted micro-elements in the fluidic network and relate the two layers to enable pneumatic control of fluid conditions in the microfluidic network.

    Abstract translation: 一种形成包含微流体网络的气动微流体装置的方法，微流体网络包括微粒或微长度中空流动元件形式的微元件，以及包括气动微通道和微阀特征的气动网络 使得膜阀能够操作以控制微流体网络中的流体状况，包括步骤（a）形成两个部分，其中形成有微流体通道的流体层和气动层，气动微通道和微阀特征 形成，每个具有在层的平面中刚性的背衬，（b）提供中间弹性膜，和（c）将两层永久地粘合到膜的相对侧，将膜永久粘合到 流体层有效地将一组插入的微元件永久地包围在流体网络中并且使两个层相关联以使得能够对流体进行气动控制 d微流控网络中的条件。

    Microfluidic assay assemblies and methods of manufacture
    5.
    发明授权
    Microfluidic assay assemblies and methods of manufacture 有权

    公开(公告)号：US10414143B2

    公开(公告)日：2019-09-17

    申请号：US16118985

    申请日：2018-08-31

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam

    IPC: B32B37/10 ,  B32B37/00 ,  B32B37/02 ,  B01L3/00 ,  G01N21/05 ,  G01N21/64 ,  G01N33/543 ,  G01N21/03 ,  G01N35/00

    Abstract: A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.

    Microfluidic devices and methods of manufacture and use
    6.
    发明授权
    Microfluidic devices and methods of manufacture and use 有权

    公开(公告)号：US10252263B2

    公开(公告)日：2019-04-09

    申请号：US14955785

    申请日：2015-12-01

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

    IPC: B29C65/00 ,  B29C65/02 ,  B32B37/00 ,  B32B38/00 ,  B65C3/26 ,  B32B38/04 ,  B32B27/00 ,  G01N33/00 ,  G01N15/06 ,  G01N33/48 ,  B01L3/00 ,  C12M1/34 ,  C12M3/00 ,  F15C1/06 ,  G01N21/05 ,  G01N21/64 ,  G01N33/543 ,  B32B37/14 ,  B32B37/18 ,  G01N21/03

    Abstract: Microfluidic devices are provided for conducting fluid assays, for example biological assays, that have the ability to move fluids through multiple channels and pathways in a compact, efficient, and low cost manner. Discrete flow detection elements, preferably extremely short hollow flow elements, with length preferably less than 700 micron, preferably less than 500 micron, and internal diameter preferably of between about 50+/−25 micron, are provided with capture agent, and are inserted into microfluidic channels by tweezer or vacuum pick-and-place motions at fixed positions in which they are efficiently exposed to fluids for conducting assays. Close-field electrostatic attraction is employed to define the position of the elements and enable ready withdrawal of the placing instruments. The microfluidic devices feature flow elements, channels, valves, and on-board pumps that are low cost to fabricate accurately, are minimally invasive to the fluid path and when implemented for the purpose, can produce multiplex assays on a single portable assay cartridge (chip) that have low coefficients of variation. Novel methods of construction, assembly and use of these features are presented, including co-valent bonding of selected regions of faces of surface-activatable bondable materials, such as PDMS to PDMS and PDMS to glass, while contiguous portions of one flexible sheet completes and seals flow channels, fixes the position of inserted analyte-detection elements in the channels, especially short hollow flow elements through which sample and reagent flow, and other portions form flexible valve membranes and diaphragms of pumps. A repeated make-and-break-contact manufacturing protocol prevents such bonding to interfere with moving the integral valve diaphragm portions from their valve seats defined by the opposed sheet member, which the flexible sheet material engages. Preparation of two subassemblies, each having a backing of relatively rigid material, followed by their assembly face-to-face in a permanent bond is shown. Hollow detection flow elements are shown fixed in channels, that provide by-pass flow paths of at least 50% of the flow capacity through the elements; in preferred implementations, as much as 100% or more. Metallized polyester film is shown to have numerous configurations and advantages in non-permanently bonded constructions. A method of preparing detection elements for an assay comprises batch coating detection elements, or hollow flow elements by mixing and picking and placing the elements in flow channels of a microfluidic device, capturing the flow elements by bonding two opposed layers while sealing the flow channels.

    Microfluidic assay assemblies and methods of manufacture
    9.
    发明授权
    Microfluidic assay assemblies and methods of manufacture 有权

    公开(公告)号：US11292237B2

    公开(公告)日：2022-04-05

    申请号：US16570127

    申请日：2019-09-13

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam

    IPC: B32B37/10 ,  B32B37/00 ,  B32B37/02 ,  B01L3/00 ,  G01N21/05 ,  G01N21/64 ,  G01N33/543 ,  G01N21/03 ,  G01N35/00

    Abstract: A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.

    PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
    10.
    发明授权
    PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use 有权

    公开(公告)号：US10209250B2

    公开(公告)日：2019-02-19

    申请号：US15638526

    申请日：2017-06-30

    Applicant: CyVek, Inc.

    Inventor： Martin A. Putnam ,  John H. Leamon ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

    IPC: G01N33/543 ,  B01L3/00 ,  G01N21/05 ,  G01N21/64 ,  G01N21/03 ,  G01N35/00

    Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent. In some embodiments, the network may have at least two microfluidic channels, each channel of the two channels arranged to receive portions of the same fluid and to be fluidicly isolatable from each other, the device having at least two micro-particles disposed in fixed position in the network channels, the micro-particles being functionalized with a capture agent, one of the micro-particles in one of the channels being functionalized with an antibody or antigen capture agent and another of the micro-particles in another of the channels being functionalized with a nucleic acid capture agent. The method may also include detecting both protein and nucleic acid present in an input sample using the respectively functionalized micro particles. In some embodiments, the micro particles may be micro-length tubes or glass nano reactors.

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