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公开(公告)号：US5726014A
公开(公告)日：1998-03-10
申请号：US123936
申请日：1993-09-17
IPC分类号： C12N15/09 , C07K14/035 , C07K14/47 , C12N15/10 , C12N15/67 , C12Q1/68 , C12Q1/70 , C12P19/34 , G01N33/566
CPC分类号： C12Q1/6811 , C07K14/47 , C12N15/1048 , C12N15/67 , C12Q1/6883 , C12Q1/705
摘要： The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
摘要翻译： 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。
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公开(公告)号：US6010849A
公开(公告)日：2000-01-04
申请号：US482080
申请日：1995-06-07
CPC分类号： C12Q1/701 , C07K14/47 , C12N15/1048 , C12N15/67 , C12Q1/6811 , C12Q1/6813 , C12Q1/6876 , C12Q1/6883 , C12Q1/705 , H01L2924/14
摘要： The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
摘要翻译： 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。
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公开(公告)号：US06869765B2
公开(公告)日：2005-03-22
申请号：US09993346
申请日：2001-11-13
CPC分类号： C12Q1/701 , C07K14/47 , C12N15/1048 , C12N15/67 , C12Q1/6811 , C12Q1/6813 , C12Q1/6876 , C12Q1/6883 , C12Q1/705 , H01L2924/14 , C12Q2525/161 , C12Q2525/131 , C12Q2537/161 , C12Q2522/101 , C12Q2521/301
摘要： The present invention defines a DNA: protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
摘要翻译： 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。
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4.发明授权
公开(公告)号：US5869241A
公开(公告)日：1999-02-09
申请号：US475228
申请日：1995-06-07
CPC分类号： C12Q1/701 , C07K14/47 , C12N15/1048 , C12N15/67 , C12Q1/6811 , C12Q1/6813 , C12Q1/6876 , C12Q1/6883 , C12Q1/705 , H01L2924/14
摘要： The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
摘要翻译： 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。
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公开(公告)号：US5578444A
公开(公告)日：1996-11-26
申请号：US171389
申请日：1993-12-20
IPC分类号： C07K14/47 , C12N15/10 , C12N15/67 , C12Q1/68 , C12Q1/70 , C07H21/02 , C12N15/00 , G01N33/574
CPC分类号： C12Q1/701 , C07K14/47 , C12N15/1048 , C12N15/67 , C12Q1/6811 , C12Q1/6813 , C12Q1/6876 , C12Q1/6883 , C12Q1/705 , H01L2924/14
摘要： The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
摘要翻译： 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。
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公开(公告)号：US06384208B1
公开(公告)日：2002-05-07
申请号：US09354947
申请日：1999-07-15
IPC分类号： C07H2104
CPC分类号： C12Q1/701 , C07K14/47 , C12N15/1048 , C12N15/67 , C12Q1/6811 , C12Q1/6813 , C12Q1/6876 , C12Q1/6883 , C12Q1/705 , H01L2924/14 , C12Q2525/161 , C12Q2525/131 , C12Q2537/161 , C12Q2522/101 , C12Q2521/301
摘要： The present invention defines a DNA: protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.
摘要翻译： 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。
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公开(公告)号：US5744131A
公开(公告)日：1998-04-28
申请号：US476876
申请日：1995-06-07
IPC分类号： C07K14/47 , C12N15/09 , C12N15/10 , C12N15/67 , C12Q1/68 , C12Q1/70 , A61K31/74 , G01N33/558 , G01N33/566
CPC分类号： C12N15/67 , C07K14/47 , C12N15/1048 , C12Q1/6811 , C12Q1/6883 , C12Q1/705
摘要： The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.
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公开(公告)号：US5716780A
公开(公告)日：1998-02-10
申请号：US484499
申请日：1995-06-07
CPC分类号： C12N15/67 , C07K14/47 , C12N15/1048 , C12Q1/6811 , C12Q1/6883 , C12Q1/705
摘要： The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.
摘要翻译： 本发明定义了一种用于筛选合成或生物化合物文库以测定其结合特异性DNA测试序列的能力的测定法。 该测定还可用于确定DNA结合分子对于任何特定DNA序列的序列特异性和相对DNA结合亲和力。 本文还描述了测定的潜在应用，包括：1）通过大量筛选合成或生物化合物（即发酵液）文库来检测铅化合物或新药物; 2）由使用该测定法确定序列特异性的分子的同源或异源亚基组成的序列特异性DNA结合药物的设计; 和3）使用通过测定作为共价连接的部分确定序列特异性的分子，以有助于核酸或其它大分子聚合物与核酸序列的结合。
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公开(公告)号：US5738990A
公开(公告)日：1998-04-14
申请号：US475221
申请日：1995-06-07
CPC分类号： C12N15/67 , C07K14/47 , C12N15/1048 , C12Q1/6811 , C12Q1/6883 , C12Q1/705
摘要： The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.
摘要翻译： 本发明定义了一种用于筛选合成或生物化合物文库以测定其结合特异性DNA测试序列的能力的测定法。 该测定还可用于确定DNA结合分子对于任何特定DNA序列的序列特异性和相对DNA结合亲和力。 本文还描述了测定的潜在应用，包括：1）通过大量筛选合成或生物化合物（即发酵液）文库来检测铅化合物或新药物; 2）由使用该测定法确定序列特异性的分子的同源或异源亚基组成的序列特异性DNA结合药物的设计; 和3）使用通过测定作为共价连接的部分确定序列特异性的分子，以有助于核酸或其它大分子聚合物与核酸序列的结合。
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10.发明授权
公开(公告)号：US5693463A
公开(公告)日：1997-12-02
申请号：US996783
申请日：1992-12-23
IPC分类号： C07K14/47 , C12N15/09 , C12N15/10 , C12N15/67 , C12Q1/68 , C12Q1/70 , C07H21/02 , C12N15/00 , G01N33/574
CPC分类号： C12N15/67 , C07K14/47 , C12N15/1048 , C12Q1/6811 , C12Q1/6883 , C12Q1/705
摘要： The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.
摘要翻译： 本发明定义了一种用于筛选合成或生物化合物文库以测定其结合特异性DNA测试序列的能力的测定法。 该测定还可用于确定DNA结合分子对于任何特定DNA序列的序列特异性和相对DNA结合亲和力。 本文还描述了测定的潜在应用，包括：1）通过大量筛选合成或生物化合物（即发酵液）文库来检测铅化合物或新药物; 2）由使用该测定法确定序列特异性的分子的同源或异源亚基组成的序列特异性DNA结合药物的设计; 和3）使用通过测定作为共价连接的部分确定序列特异性的分子，以有助于核酸或其它大分子聚合物与核酸序列的结合。
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