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    Polymerizable catonic peptide monomers and polymers
    1.
    发明授权
    Polymerizable catonic peptide monomers and polymers 有权
    可聚合的共聚肽单体和聚合物

    公开(公告)号：US08981025B2

    公开(公告)日：2015-03-17

    申请号：US13369689

    申请日：2012-02-09

    申请人： Dana Craig Bookbinder ,  Paul Ernest Gagnon, Jr. ,  Arthur Winston Martin ,  Jodelle Karen Nelson ,  Shawn Michael O'Malley

    发明人： Dana Craig Bookbinder ,  Paul Ernest Gagnon, Jr. ,  Arthur Winston Martin ,  Jodelle Karen Nelson ,  Shawn Michael O'Malley

    IPC分类号： C08F222/38 ,  C08G69/10 ,  C08F220/70 ,  C08F220/58

    CPC分类号： C08F222/38 ,  C08F220/58 ,  C08F220/70 ,  C08G69/10

    摘要： Disclosed herein are functionalized cationic peptide monomers: peptide chains of two or more positively charged amino acids chosen from lysine or arginine, or derivatives thereof, which are functionalized, meaning that they are bound to one or more polymerization moieties. The functionalized cationic peptide monomers can be described by the formula Z-Xaan-Z1n1 wherein Z and Z1 are polymerization moieties and n1 is an integer of 0 or 1; Xaa is each independently an amino acid Lys or Arg and n is an integer from 2 to 10 and wherein at least one Xaa amino acid of Xaa is Lys, wherein the carboxyl terminus of the amino acid sequence is amidated. Functionalized cationic peptide monomers can be combined and polymerized to form cell culture surfaces.

    摘要翻译： 本文公开了官能化的阳离子肽单体：选自赖氨酸或精氨酸或其衍生物的两个或更多个带正电荷的氨基酸的肽链，其被官能化，意味着它们与一个或多个聚合部分结合。 官能化阳离子肽单体可以用式Z-Xanan-Z1n1描述，其中Z和Z 1是聚合部分，n 1是0或1的整数; Xaa各自独立地是氨基酸Lys或Arg，n是2至10的整数，并且其中Xaa的至少一个Xaa氨基酸是Lys，其中氨基酸序列的羧基末端被酰胺化。 官能化阳离子肽单体可以组合并聚合以形成细胞培养物表面。

    POLYMERIZABLE CATONIC PEPTIDE MONOMERS AND POLYMERS
    2.
    发明申请
    POLYMERIZABLE CATONIC PEPTIDE MONOMERS AND POLYMERS 有权
    可聚合的肽类单体和聚合物

    公开(公告)号：US20120208972A1

    公开(公告)日：2012-08-16

    申请号：US13369689

    申请日：2012-02-09

    申请人： Dana Craig Bookbinder ,  Paul Ernest Gagnon, JR. ,  Arthur Winston Martin ,  Jodelle Karen Nelson ,  Shawn Michael O'Malley

    发明人： Dana Craig Bookbinder ,  Paul Ernest Gagnon, JR. ,  Arthur Winston Martin ,  Jodelle Karen Nelson ,  Shawn Michael O'Malley

    IPC分类号： C08F222/38

    CPC分类号： C08F222/38 ,  C08F220/58 ,  C08F220/70 ,  C08G69/10

    摘要： Disclosed herein are functionalized cationic peptide monomers: peptide chains of two or more positively charged amino acids chosen from lysine or arginine, or derivatives thereof, which are functionalized, meaning that they are bound to one or more polymerization moieties. The functionalized cationic peptide monomers can be described by the formula Z-Xaan-Z1n1 wherein Z and Z1 are polymerization moieties and n1 is an integer of 0 or 1; Xaa is each independently an amino acid Lys or Arg and n is an integer from 2 to 10 and wherein at least one Xaa amino acid of Xaa is Lys, wherein the carboxyl terminus of the amino acid sequence is amidated. Functionalized cationic peptide monomers can be combined and polymerized to form cell culture surfaces.

    摘要翻译： 本文公开了官能化的阳离子肽单体：选自赖氨酸或精氨酸或其衍生物的两个或更多个带正电荷的氨基酸的肽链，其被官能化，意味着它们与一个或多个聚合部分结合。 官能化阳离子肽单体可以用式Z-Xanan-Z1n1描述，其中Z和Z 1是聚合部分，n 1是0或1的整数; Xaa各自独立地是氨基酸Lys或Arg，n是2至10的整数，并且其中Xaa的至少一个Xaa氨基酸是Lys，其中氨基酸序列的羧基末端被酰胺化。 官能化阳离子肽单体可以组合并聚合以形成细胞培养物表面。

    Modified substrates for protection of peptide-immobilized surfaces from gamma radiation degradation
    6.
    发明授权
    Modified substrates for protection of peptide-immobilized surfaces from gamma radiation degradation 有权

    公开(公告)号：US10144914B2

    公开(公告)日：2018-12-04

    申请号：US13032166

    申请日：2011-02-22

    申请人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    发明人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    IPC分类号： C12N5/00

    摘要： In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of anti-oxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation.

    MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION
    7.
    发明申请
    MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION 审中-公开
    用于保护肽固定表面的改性基质从伽马辐射降解

    公开(公告)号：US20110207219A1

    公开(公告)日：2011-08-25

    申请号：US13032166

    申请日：2011-02-22

    申请人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    发明人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0068 ,  C12N2533/20 ,  C12N2533/50

    摘要： In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of anti-oxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation.

    摘要翻译： 在本说明书和权利要求书中，公开了三种修饰的细胞培养基质，用于保护细胞培养技术中使用的肽模拟表面。 该方法能够保留与表面缀合的生物活性物质的功能。 特别地，玻连蛋白肽片段Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2能够促进未分化人的生长和增殖 使用3种不同的修饰的细胞培养物底物进行稳定后的胚胎干细胞以防止γ照射。 本发明公开的改性底物是（i）抗氧化剂分子的共价连接（通过封闭步骤）; （ii）使用包含抗氧化剂和稳定剂的涂层，其包含糖调节剂，糖，碳水化合物，聚（氨基酸），肽和亲水性聚合物; 和（iii）第三种方法，其中用于促进人类胚胎干细胞生长和增殖的生物活性序列中所含的相同甲硫氨酸也用作牺牲涂层以保护相同缀合序列免受γ照射的损伤。

    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA
    9.
    发明申请
    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA 有权
    用于化学定义培养基培养细胞的（甲基）丙烯酸酯表面

    公开(公告)号：US20090191632A1

    公开(公告)日：2009-07-30

    申请号：US12362974

    申请日：2009-01-30

    申请人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    发明人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    IPC分类号： C12N5/02

    CPC分类号： C12N5/0606 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50

    摘要： Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

    摘要翻译： 能够支持未分化的人类胚胎干细胞培养在化学成分确定的培养基中的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀（甲基）丙烯酸酯层共轭的肽。 可溶胀的（甲基）丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯，2-羧基丙烯酸烯丙酯和四（乙二醇）二甲基丙烯酸酯的组合物中的单体聚合而形成。 缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列，其中n是0至3的整数，并且其中Xaa是任何氨基酸。 此外，本文公开了可通过γ辐射灭菌的可溶胀（甲基）丙烯酸酯合成表面。