公开(公告)号：US09382563B2

公开(公告)日：2016-07-05

申请号：US14097168

申请日：2013-12-04

Applicant: Danisco US Inc.

Inventor： Toby M. Baldwin ,  Benjamin S. Bower ,  Nigel Dunn-Coleman ,  Suzanne E. Lantz ,  Michael J. Pepsin

IPC: C12N9/34 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C12P21/06 ,  C07H21/04 ,  C12P19/14 ,  C12N1/14 ,  C12N9/30 ,  C12P19/20 ,  C12P19/24 ,  C12P19/02 ,  C12P19/04

CPC classification number: C12P19/14 ,  C12N1/14 ,  C12N9/242 ,  C12N9/2428 ,  C12P19/02 ,  C12P19/04 ,  C12P19/20 ,  C12P19/24 ,  Y02E50/17 ,  Y02P20/52

Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity.

Abstract translation: 本发明涉及丝状真菌宿主细胞，特别是有用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶的木霉宿主细胞。

公开(公告)号：US20150284756A1

公开(公告)日：2015-10-08

申请号：US14688283

申请日：2015-04-16

Applicant: Danisco US Inc.

Inventor： Toby M. Baldwin ,  Benjamin S. Bower ,  Gopal K. Chotani ,  Nigel Dunn-Coleman ,  Oreste J. Lantero ,  Suzanne Lantz ,  Michael J. Pepsin ,  Jayarama K. Shetty ,  Bruce A. Strohm

IPC: C12P19/14 ,  C12P19/02 ,  C12P19/04

CPC classification number: C12P19/14 ,  C12N1/14 ,  C12N9/242 ,  C12N9/2428 ,  C12P19/02 ,  C12P19/04 ,  C12P19/20 ,  C12P19/24 ,  Y02E50/17 ,  Y02P20/52

Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

Abstract translation: 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。

公开(公告)号：US09005947B2

公开(公告)日：2015-04-14

申请号：US14158672

申请日：2014-01-17

Applicant: Danisco US Inc.

Inventor： Nigel Dunn-Coleman ,  Michael Ward

IPC: C12N9/42 ,  C12P21/06 ,  C12P21/04 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C11D3/386 ,  C12P7/14

CPC classification number: C12N9/2437 ,  C11D3/386 ,  C11D3/38645 ,  C12N9/244 ,  C12N9/2445 ,  C12N15/1137 ,  C12N2310/11 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01021 ,  Y02E50/17

Abstract: The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same.

Abstract translation: 本发明提供了一种命名为bgl7的新型的葡糖苷酶核酸序列和相应的BGL7氨基酸序列。 本发明还提供了包含编码BGL7的核酸序列，重组BGL7蛋白的表达载体和宿主细胞及其制备方法。

公开(公告)号：US20130122569A1

公开(公告)日：2013-05-16

申请号：US13664216

申请日：2012-10-30

Applicant: Danisco US Inc.

Inventor： Toby M. Baldwin ,  Benjamin S. Bower ,  Gopal K. Chotani ,  Nigel Dunn-Coleman ,  Oreste J. Lantero, JR. ,  Suzanne Lantz ,  Michael J. Pepsin ,  Jayarama K. Shetty ,  Bruce A. Strohm

IPC: C12N9/24

CPC classification number: C12P19/14 ,  C12N1/14 ,  C12N9/242 ,  C12N9/2428 ,  C12P19/02 ,  C12P19/04 ,  C12P19/20 ,  C12P19/24 ,  Y02E50/17 ,  Y02P20/52

Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

Abstract translation: 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。

公开(公告)号：US20140127757A1

公开(公告)日：2014-05-08

申请号：US14067609

申请日：2013-10-30

Applicant: Danisco US Inc.

Inventor： Toby M. Baldwin ,  Kathleen A. Clarkson ,  Nigel Dunn-Coleman ,  Suzanne E. Lantz

IPC: C12N9/30 ,  C12P19/02 ,  C12P19/14

CPC classification number: C12N9/242 ,  C12N9/2417 ,  C12N9/2428 ,  C12N15/8245 ,  C12P7/06 ,  C12P7/10 ,  C12P19/02 ,  C12P19/04 ,  C12P19/14 ,  C12P19/22 ,  C12P21/06 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to the co-expression and production of a heterologous alpha amylase and an endogenous glucoamylase in an Aspergillus strain and enzyme compositions including the same.

Abstract translation: 本发明涉及Aspergillus菌株中的异源α淀粉酶和内源性葡糖淀粉酶的共表达和产生以及包含其的酶组合物。

公开(公告)号：US08679818B2

公开(公告)日：2014-03-25

申请号：US13717115

申请日：2012-12-17

Applicant: Danisco US Inc.

Inventor： Nigel Dunn-Coleman ,  Michael Ward

IPC: C12N9/42 ,  C12P21/06 ,  C12P21/04 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C11D3/386

CPC classification number: C12N9/2437 ,  C11D3/386 ,  C11D3/38645 ,  C12N9/244 ,  C12N9/2445 ,  C12N15/1137 ,  C12N2310/11 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01021 ,  Y02E50/17

Abstract: The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same.

Abstract translation: 本发明提供了一种命名为bgl7的新型的葡糖苷酶核酸序列和相应的BGL7氨基酸序列。 本发明还提供了包含编码BGL7的核酸序列，重组BGL7蛋白的表达载体和宿主细胞及其制备方法。

公开(公告)号：US09961909B2

公开(公告)日：2018-05-08

申请号：US14795152

申请日：2015-07-09

Applicant: Danisco US Inc.

Inventor： Nigel Dunn-Coleman ,  Michael Ward

IPC: C12N1/20 ,  A21D8/04 ,  C11D3/386 ,  C12N9/42 ,  C12P7/14 ,  C12N15/113

CPC classification number: A21D8/042 ,  C11D3/38645 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N15/1137 ,  C12N2310/11 ,  C12P7/14 ,  C12Y302/01021 ,  Y02E50/17

Abstract: The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.

公开(公告)号：US09428780B2

公开(公告)日：2016-08-30

申请号：US14688283

申请日：2015-04-16

Applicant: Danisco US Inc.

Inventor： Toby M. Baldwin ,  Benjamin S. Bower ,  Gopal K. Chotani ,  Nigel Dunn-Coleman ,  Oreste J. Lantero ,  Suzanne Lantz ,  Michael J. Pepsin ,  Jayarama K. Shetty ,  Bruce A. Strohm

IPC: C12P19/14 ,  C12N1/14 ,  C12N9/30 ,  C12N9/34 ,  C12P19/20 ,  C12P19/24 ,  C12P19/02 ,  C12P19/04

CPC classification number: C12P19/14 ,  C12N1/14 ,  C12N9/242 ,  C12N9/2428 ,  C12P19/02 ,  C12P19/04 ,  C12P19/20 ,  C12P19/24 ,  Y02E50/17 ,  Y02P20/52

Abstract: The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

Abstract translation: 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。

公开(公告)号：US20140087023A1

公开(公告)日：2014-03-27

申请号：US13924330

申请日：2013-06-21

Applicant: Danisco US Inc.

Inventor： Gang DUAN ,  Nigel Dunn-Coleman ,  Jayarma K. Shetty ,  Alex Sung ,  Ying Qian

IPC: A23J1/12

CPC classification number: A23J1/125 ,  A23J3/346 ,  A23K20/147 ,  A23L7/107 ,  A23L7/198

Abstract: The present invention relates to a process for producing a rice protein concentrate from a rice substrate comprising, enzymatically hydrolyzing a rice substrate with an enzyme having granular starch hydrolyzing (GSH) activity and a second starch hydrolyzing enzyme at a temperature at or below 72° C. and at a pH of about 3.0 to 6.5 to obtain a solubilized starch fraction and a residue fraction which includes insoluble protein, and separating the solubilized starch fraction from the residue to obtain a rice protein concentrate. The rice protein concentrate may be used in animal feed and human food formulations.

Abstract translation: 本发明涉及一种从水稻基质制备大米蛋白浓缩物的方法，包括在72℃或低于72℃的温度下，用具有颗粒状淀粉水解（GSH）活性的酶和第二淀粉水解酶酶水解水稻基质 并且在约3.0至6.5的pH下，得到溶解的淀粉级分和包含不溶性蛋白质的残余物级分，并将残留的溶解的淀粉部分分离，得到大米蛋白质浓缩物。 米蛋白浓缩物可用于动物饲料和人类食物制剂中。

公开(公告)号：US08658406B2

公开(公告)日：2014-02-25

申请号：US13920043

申请日：2013-06-17

Applicant: Danisco US Inc.

Inventor： Nigel Dunn-Coleman ,  Frits Goedegebuur ,  Michael Ward ,  Jian Yao

IPC: C12P7/06 ,  C12Q1/68 ,  C12Q1/34 ,  C12N9/42 ,  C12N15/00 ,  C12N1/20 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04 ,  C12N1/00

CPC classification number: C12N9/2437 ,  C11D3/38 ,  C11D3/38636 ,  C11D3/38645 ,  C12N9/2445 ,  C12N15/1137 ,  C12N2310/11 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12Y302/01004 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

Abstract translation: 本发明提供了一种命名为egl7的新型内切葡聚糖酶核酸序列和相应的EGVII氨基酸序列。 本发明还提供了包含编码EGVII的核酸序列，重组EGVII蛋白的表达载体和宿主细胞及其制备方法。