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公开(公告)号：US20050191646A1
公开(公告)日：2005-09-01
申请号：US10961341
申请日：2004-10-07
申请人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
发明人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G16B25/00 , G16B30/00 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
摘要翻译： 本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。 所述方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。 来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。 样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。 本发明还提供了一种终止标记核酸的方法。 在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。 因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。 在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。
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公开(公告)号：US20050158772A1
公开(公告)日：2005-07-21
申请号：US11021367
申请日：2004-12-23
申请人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
发明人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G16B25/00 , G16B30/00 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
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公开(公告)号：US06344316B1
公开(公告)日：2002-02-05
申请号：US08882649
申请日：1997-06-25
申请人： David J. Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen T. Cronin , Danny Lee , Huu M. Tran , Hajime Matsuzaki
发明人： David J. Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen T. Cronin , Danny Lee , Huu M. Tran , Hajime Matsuzaki
IPC分类号： C12Q168
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G06F19/20 , G06F19/22 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
摘要翻译： 本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。 所述方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。 来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。 样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。 本发明还提供了一种终止标记核酸的方法。 在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。 因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。 在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。
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公开(公告)号：US08470531B2
公开(公告)日：2013-06-25
申请号：US12633759
申请日：2009-12-08
申请人： Shoulian Dong , Junko Stevens , Danny Lee
发明人： Shoulian Dong , Junko Stevens , Danny Lee
CPC分类号： C12Q1/6846 , C12N15/115 , C12N2310/16 , C12P19/34 , C12Q1/6848 , G01N21/6486 , C12Q2549/125 , C12Q2527/127 , C12Q2527/107
摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.
摘要翻译： 本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不需要的扩增产物，有时称为二级扩增产物或假副产物。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。 还提供了用于加速某些公开方法的执行的套件。
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公开(公告)号：US20070295089A1
公开(公告)日：2007-12-27
申请号：US11761112
申请日：2007-06-11
申请人： Steven Velinsky , Danny Lee
发明人： Steven Velinsky , Danny Lee
CPC分类号： G01C17/30
摘要： A three-dimensional non-contacting angular motion sensor, based on magnetometry, has been developed for velocity feedback in the ball wheel mechanism, which serves as the drivetrain for a class of omnidirectional mobile platforms. More generally, this scheme tracks rigid-body rotation about a fixed point with an undefined axis of rotation. The approach involves tracking the time-varying magnetic field of a permanent disc magnet, embedded at the center of the sphere. This data is then used to determine the absolute orientation of the magnet axis. Finally, an approach based on the natural invariants of rigid-body motion is used to determine the instantaneous axis of rotation and the angular speed of the sphere about this axis.
摘要翻译： 基于磁力计的三维非接触式角运动传感器已被开发用于球轮机构中的速度反馈，其用作一类全向移动平台的传动系。 更一般地，该方案跟踪具有未定义的旋转轴线的固定点周围的刚体旋转。 该方法包括跟踪嵌入球体中心的永磁盘磁体的时变磁场。 然后将该数据用于确定磁体轴的绝对方向。 最后，使用基于刚体运动的自然不变量的方法来确定旋转的瞬时轴线和围绕该轴的球体的角速度。
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公开(公告)号：US06933453B1
公开(公告)日：2005-08-23
申请号：US10968112
申请日：2004-10-20
申请人： Danny Lee
发明人： Danny Lee
CPC分类号： H01H23/025 , H01H2219/0622
摘要： A switch includes a transparent housing, a control member, and several light emitting elements; the control member is held in and pivoted to the housing with a depressed portion being within an opening at one end of the housing; the housing has two side compartments on two sides of a middle compartment, and an annular protrusion around the opening on an outer side thereof; the light emitting elements are held in the side compartments of the housing with legs thereof projecting outwards through other end of the housing such that the switch is available with the light emitting elements therein, and such that a circle of light will show on the annular protrusion around the control member when the light emitting elements are powered.
摘要翻译： 开关包括透明壳体，控制构件和若干发光元件; 控制构件被保持在壳体中并且枢转到壳体，凹部在壳体的一端处于开口内; 壳体在中间隔室的两侧具有两个侧隔室，在其外侧上具有围绕开口的环形突起; 发光元件被保持在壳体的侧隔室中，其腿部通过壳体的另一端向外突出，使得开关可用于其中的发光元件，并且使得在环形突起上将示出一圈光 当发光元件被供电时，围绕控制构件。
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公开(公告)号：US08575071B2
公开(公告)日：2013-11-05
申请号：US12952563
申请日：2010-11-23
申请人： Patrick Lau , Danny Lee
发明人： Patrick Lau , Danny Lee
IPC分类号： C40B50/12
CPC分类号： C12N15/1068 , C12Q1/6855 , C40B50/06 , C12Q2537/163 , C12Q2533/107 , C12Q2525/301 , C12Q2525/197
摘要： Provided herein is a method of reducing adapter dimer formation comprising contacting a sample comprising target nucleic acid sequences with 5′ and 3′ adapters in the presence of one or more hairpin oligonucleotides. Also provided is a method of preparing a library of nucleic acid sequences comprising contacting first adapter oligonucleotides with a sample comprising target nucleic acid sequences under conditions to form first ligation products, contacting the sample with one or more hairpin oligonucleotides that binds to the first adapter oligonucleotides, and contacting the sample with second adapter oligonucleotides under conditions to bind to the first ligation products and form second ligation products, wherein the second ligation products form the library of nucleic acid sequences.
摘要翻译： 本文提供了减少适配体二聚体形成的方法，包括在一种或多种发夹寡核苷酸存在下使包含靶核酸序列的样品与5'和3'衔接子接触。 还提供了制备核酸序列文库的方法，包括在条件下使第一衔接子寡核苷酸与包含靶核酸序列的样品接触以形成第一连接产物，使样品与一种或多种结合第一衔接子寡核苷酸的发夹寡核苷酸接触 并且在与第一连接产物结合的条件下使样品与第二衔接子寡核苷酸接触并形成第二连接产物，其中第二连接产物形成核酸序列文库。
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公开(公告)号：US5599442A
公开(公告)日：1997-02-04
申请号：US665170
申请日：1996-06-14
申请人： Colin McRae , Danny Lee , Peter Avotins
发明人： Colin McRae , Danny Lee , Peter Avotins
CPC分类号： B03D1/012 , B03D2201/02 , B03D2203/02
摘要： New and improved processes and compositions for use in flotation recovery of metal values from base metal zinc sulfide ores are disclosed. The collector compositions for froth flotation of metal sulfide minerals comprise the combination of combination of 1) at least one allylalkylthionocarbamate compound having the formula ##STR1## wherein R is a C.sub.2 to C.sub.8 alkyl radical, preferably R is an isobutyl group, and 2) a xanthate having the formula ##STR2## wherein R' is a C.sub.2 to C.sub.8 alkyl radical, preferably a C.sub.4 radical. More preferably, the C.sub.4 radical of the xanthate is a butyl or isopropyl group. Preferably, the concentration of allylalkylthionocarbamate to xanthate of a ratio of from about 1:99 to about 50:50 percent by weight.
摘要翻译： 公开了用于从贱金属硫化锌矿石浮选回收金属值的新的和改进的方法和组合物。 用于泡沫浮选金属硫化物矿物的收集器组合物包括1）至少一种具有式“IMAGE”的烯丙基烷基硫代氨基甲酸酯化合物的组合，其中R是C 2至C 8烷基，优选R是异丁基，以及2） 具有式“IMAGE”的黄原酸酯，其中R'是C 2至C 8烷基，优选C 4基团。 更优选地，黄原酸酯的C4基团是丁基或异丙基。 优选地，烯丙基烷基硫代氨基甲酸酯对黄原酸酯的浓度为约1:99至约50:50重量％。
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公开(公告)号：US07868610B2
公开(公告)日：2011-01-11
申请号：US11761112
申请日：2007-06-11
申请人： Steven A. Velinsky , Danny Lee
发明人： Steven A. Velinsky , Danny Lee
IPC分类号： G01B7/30
CPC分类号： G01C17/30
摘要： A motion tracking sensor apparatus configured for real-time, three-dimensional angular motion tracking that can determine the absolute orientation, axis of rotation and angular speed of a body rotating about a point, which is fixed relative to the sensor, without contacting the rotating body. The sensor obtains measurements of the three-dimensional magnetic field of a dipole fixed on the body. In one embodiment, a permanent magnet is embedded in the center of the rotating body, and the time-varying magnetic field is measured as the magnet rotates, and then the instantaneous magnet orientation from the field data is determined. The apparatus is particularly suited for use with devices that are based on a sphere rotating in a cradle such as spherical motors or spherical variable transmissions.
摘要翻译： 一种运动跟踪传感器装置，被配置用于实时三维角运动跟踪，其可以确定绕相对于传感器固定的点旋转的绝对取向，旋转轴线和角速度，而不接触旋转 身体。 传感器获得固定在人体上的偶极子的三维磁场的测量结果。 在一个实施例中，将永磁体嵌入在旋转体的中心，并且当磁体旋转时测量时变磁场，然后确定来自场数据的瞬时磁体取向。 该装置特别适用于基于在支架中旋转的球体的装置，例如球面马达或球面可变传动装置。
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公开(公告)号：US06670727B2
公开(公告)日：2003-12-30
申请号：US10021241
申请日：2001-12-19
申请人： Danny Lee , Jui-Pin Huang
发明人： Danny Lee , Jui-Pin Huang
IPC分类号： H01H326
CPC分类号： H01H3/503 , H01H23/025 , H01H43/00 , Y10T307/944 , Y10T307/957
摘要： A timed switch has a housing member, an output connecting leg connected to an appliance, and a circuit board including both a timing device and an electromagnet. The output connecting leg is electrically connected to the circuit board, and has a front end movable member disposed behind the front wall of the housing member. A control button is pivoted to the outer side of the front wall from a middle with an upper and a lower pushing protrusions passing through the front wall to abut an upper part and a lower part of the movable member respectively. A first, and a second input connecting legs are provided, which are connected to the power, and the second further to the circuit board. A power-on end of the button is pushed to make the upper part, and the lower part of the movable member to contact the first leg and the electromagnet respectively; thus, the appliance and the circuit board are energized, and the electromagnet attracts the lower part of the movable member thereto so as to make the upper part continue to contact the first leg to complete the circuit. At the same time, the timing device is actuated such that the appliance can be automatically turned off at a preselected time after it has been actuated.
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