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公开(公告)号：US6136040A
公开(公告)日：2000-10-24
申请号：US256546
申请日：1999-02-24
IPC分类号： C07K14/50 , C12N15/85 , A01K67/027 , C12N15/00
CPC分类号： C12N15/8509 , A01K67/0276 , C07K14/50 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0375 , C12N2800/30
摘要： A unique animal model is disclosed which is useful for studying the role of FGF-9 activity in cardiovascular diseases. To provide this animal model, the FGF-9 gene is disrupted in mice, that is, null mutation in the mouse FGF-9 gene is engineered. This knockout mouse was produced by deleting the sequences immediately downstream of the initiation methionine in exon 1. Analysis of the FGF-9 null embryos demonstrates perinatal lethality with apparent pathology in lung and cardiac tissue.
摘要翻译： 公开了一种独特的动物模型，可用于研究FGF-9活性在心血管疾病中的作用。 为了提供这种动物模型，FGF-9基因在小鼠中被破坏，即小鼠FGF-9基因中的无效突变是被工程化的。 通过删除外显子1中起始甲硫氨酸的紧邻下游的序列产生该敲除小鼠.FGF-9无效胚胎的分析显示在肺和心脏组织中具有明显病理学的围产期致死性。
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公开(公告)号：US06942964B1
公开(公告)日：2005-09-13
申请号：US09610935
申请日：2000-07-06
CPC分类号： C12Q1/6813 , C12Q2565/137 , C12Q2527/125
摘要： A composition suitable for formulation of an enzymatic reaction mixture, the composition comprising a reaction component essential for an ex-vivo non-polymerase enzymatic reaction in which a substrate is catalyzed by an enzyme in a reaction mixture to form a product, and a tracer compatible with the enzyme, the composition being substantially free of the substrate.
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公开(公告)号：US09528143B2
公开(公告)日：2016-12-27
申请号：US11734616
申请日：2007-04-12
CPC分类号： C12Q1/6813 , C12Q2565/137 , C12Q2527/125
摘要： A composition suitable for formulation of an enzymatic reaction mixture, the composition comprising a reaction component essential for an ex-vivo non-polymerase enzymatic reaction in which a substrate is catalyzed by an enzyme in a reaction mixture to form a product, and a tracer compatible with the enzyme, the composition being substantially free of the substrate.
摘要翻译： 一种适于制备酶反应混合物的组合物，该组合物包含对于在反应混合物中催化底物以形成产物的离体非聚合酶酶反应所必需的反应组分，以及示踪剂相容性 与该酶组合物基本上不含底物。
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4.发明授权Cells expressing a substantial number of surface high affinity HBGF receptors but relatively few low affinity HBGF binding sites and system for assaying binding to HBGF receptor 失效表达大量表面高亲和力HBGF受体但相对较少的低亲和力HBGF结合位点的细胞和用于测定与HBGF受体结合的系统
公开(公告)号：US5270197A
公开(公告)日：1993-12-14
申请号：US631717
申请日：1990-12-20
申请人： Avner Yayon , David M. Ornitz , Michael Klagsbrun , Philip Leder
发明人： Avner Yayon , David M. Ornitz , Michael Klagsbrun , Philip Leder
IPC分类号： C07K14/50 , C07K14/71 , C12N9/16 , G01N33/50 , G01N33/74 , C12N5/10 , C12N15/85 , C12P21/00 , G01N33/566
CPC分类号： G01N33/5008 , C07K14/503 , C07K14/71 , C12N9/16 , G01N33/74 , C07K2319/034 , C07K2319/32 , C07K2319/61 , G01N2500/00
摘要： A homogeneous population of cells having on average (1) a number of cell surface low-affinity heparin-binding growth factor (HBGF) sites per cell less than 20% of the number of such binding sites found on wild-type CHO-K1 cells (ATCC Accession No. CCL61), and at least three times the number of cell surface high-affinity HBGF receptors per cell found on such CHO-K1 cells; and an assay system utilizing such cells.
摘要翻译： 平均每个细胞的细胞表面平均少量细胞表面低亲和力肝素结合生长因子（HBGF）位点的均匀群体少于在野生型CHO-K1细胞上发现的这种结合位点数目的20％ （ATCC登录号CCL61），并且在这种CHO-K1细胞上发现的每个细胞的细胞表面高亲和力HBGF受体的数目的至少三倍; 以及利用这种细胞的测定系统。
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5.发明授权
公开(公告)号：US5789182A
公开(公告)日：1998-08-04
申请号：US166717
申请日：1993-12-14
CPC分类号： G01N33/5008 , C07K14/503 , C07K14/71 , C12N9/16 , G01N33/74 , C07K2319/034 , C07K2319/32 , C07K2319/61 , G01N2500/00
摘要： A homogeneous population of cells having on average (1) a number of cell surface low-affinity heparin-binding growth factor (HBGF) sites per cell less than 20% of the number of such binding sites found on wild-type CHO-K1 cells (ATCC Accession No. CCL61), and at least three times the number of cell surface high-affinity HBGF receptors per cell found on such CHO-K1 cells; and an assay system utilizing such cells.
摘要翻译： 平均每个细胞的细胞表面平均少量细胞表面低亲和力肝素结合生长因子（HBGF）位点的均匀群体少于在野生型CHO-K1细胞上发现的这种结合位点数目的20％ （ATCC登录号CCL61），并且在这种CHO-K1细胞上发现的每个细胞的细胞表面高亲和力HBGF受体的数目的至少三倍; 以及利用这种细胞的测定系统。
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公开(公告)号：US5733893A
公开(公告)日：1998-03-31
申请号：US404655
申请日：1995-03-15
申请人： David M. Ornitz
发明人： David M. Ornitz
IPC分类号： G01N33/15 , A61K31/70 , A61K31/7028 , A61P9/00 , A61P35/00 , A61P43/00 , C07H15/04 , C12N15/09 , C12Q1/02 , G01N33/50 , A61K47/36 , A61K31/715 , A61K31/725 , C07H3/06
CPC分类号： G01N33/5011 , G01N33/5008 , G01N33/502 , G01N2333/61 , G01N2333/71
摘要： Disclosed are fibroblast growth factor (FGF) binding and FGF receptor activation, and a method of identifying small molecular weight compounds that interact with FGF to modulate its activity such as, e.g., activators and inhibitors. Illustrative small oligosaccharides, namely di- and tri-saccharides, are shown to be effective modulators of FGF binding and FGF receptor activation.
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公开(公告)号：US20080160518A1
公开(公告)日：2008-07-03
申请号：US11734616
申请日：2007-04-12
CPC分类号： C12Q1/6813 , C12Q2565/137 , C12Q2527/125
摘要： A composition suitable for formulation of an enzymatic reaction mixture, the composition comprising a reaction component essential for an ex-vivo non-polymerase enzymatic reaction in which a substrate is catalyzed by an enzyme in a reaction mixture to form a product, and a tracer compatible with the enzyme, the composition being substantially free of the substrate.
摘要翻译： 一种适于制备酶反应混合物的组合物，该组合物包含对于在反应混合物中催化底物以形成产物的离体非聚合酶酶反应所必需的反应组分，以及示踪剂相容性 与该酶组合物基本上不含底物。
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公开(公告)号：US07169584B2
公开(公告)日：2007-01-30
申请号：US11058663
申请日：2005-02-15
CPC分类号： C12Q1/6813 , C12Q2565/137 , C12Q2527/125
摘要： A composition suitable for formulation of an enzymatic reaction mixture, the composition comprising a reaction component essential for an ex-vivo non-polymerase enzymatic reaction in which a substrate is catalyzed by an enzyme in a reaction mixture to form a product, and a tracer compatible with the enzyme, the composition being substantially free of the substrate.
摘要翻译： 一种适于制备酶反应混合物的组合物，该组合物包含对于在反应混合物中催化底物以形成产物的离体非聚合酶酶反应所必需的反应组分，以及示踪剂相容性 与该酶组合物基本上不含底物。
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公开(公告)号：US5891655A
公开(公告)日：1999-04-06
申请号：US839186
申请日：1997-04-23
申请人： David M. Ornitz
发明人： David M. Ornitz
IPC分类号： G01N33/15 , A61K31/70 , A61K31/7028 , A61P9/00 , A61P35/00 , A61P43/00 , C07H15/04 , C12N15/09 , C12Q1/02 , G01N33/50 , G01N33/567
CPC分类号： G01N33/5011 , G01N33/5008 , G01N33/502 , G01N2333/61 , G01N2333/71
摘要： Disclosed are fibroblast growth factor (FGF) binding and FGF receptor activation, and a method of identifying small molecular weight compounds that interact with FGF to modulate its activity such as, e.g., activators and inhibitors. Illustrative small oligosaccharides, namely di- and tri-saccharides, are shown to be effective modulators of FGF binding and FGF receptor activation.
摘要翻译： 公开了成纤维细胞生长因子（FGF）结合和FGF受体活化，以及鉴定与FGF相互作用以调节其活性的小分子量化合物的方法，例如活化剂和抑制剂。 示例性的小寡糖，即二糖和三糖被证明是FGF结合和FGF受体活化的有效调节剂。
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