公开(公告)号：US5489988A

公开(公告)日：1996-02-06

申请号：US368502

申请日：1995-01-03

申请人： Donald E. Ackley ,  Michael Krihak ,  Chan-Long Shieh

发明人： Donald E. Ackley ,  Michael Krihak ,  Chan-Long Shieh

IPC分类号： G01N21/78 ,  G02B6/13 ,  G02B6/26 ,  G02B6/30 ,  G02B6/36 ,  G02B6/42 ,  G01N21/00 ,  G02B6/00

CPC分类号： G01N21/783 ,  G02B6/13 ,  G02B6/262 ,  G02B6/30 ,  G02B6/4214

摘要： A sensor (10,30,40,50,70) for detecting chemicals and changes in the surrounding environment utilizes a sol-gel sensor element (14,16,17,54,56,57) containing a chemical indicator. Grooves (12,13,24,52,53) are formed in a substrate (11,51). The grooves are filled with a sol-gel material having a chemical indicator, and the sol-gel is cured to adhere to the substrate (11,51). The grooves (12,13,24,52,53) are formed to facilitate optically coupling a fiber optic cable (46) to the sol-gel sensor element. Light is coupled from the fiber optic cable (46) to the sol-gel sensor element (14,16,17,54,56,57).

摘要翻译： 用于检测化学物质和周围环境变化的传感器（10,30,40,50,70）利用含有化学指示剂的溶胶 - 凝胶传感器元件（14,16,17,54,56,57）。 沟槽（12,13,24,52,53）形成在衬底（11,51）中。 用具有化学指示剂的溶胶 - 凝胶材料填充凹槽，并且溶胶 - 凝胶固化以粘附到基底（11,51）上。 凹槽（12,13,24,52,53）形成为便于将光纤电缆（46）光学耦合到溶胶 - 凝胶传感器元件。 光从光纤电缆（46）耦合到溶胶 - 凝胶传感器元件（14,16,17,54,56,57）。

公开(公告)号：US5945286A

公开(公告)日：1999-08-31

申请号：US956676

申请日：1997-10-23

申请人： Michael Krihak ,  Chan-Long Shieh

发明人： Michael Krihak ,  Chan-Long Shieh

IPC分类号： C12Q1/68

CPC分类号： B82Y30/00 ,  B82Y5/00 ,  C12Q1/6825 ,  B01J2219/00653

摘要： A molecular detection apparatus including an electrode, a peptide nucleic acid probe covalently bonded to the electrode and a protective layer covering portions of the electrode not having attached probes which prevents oxidation/reduction of intercalator molecules.

摘要翻译： 一种分子检测装置，包括电极，共价键合到电极上的肽核酸探针和覆盖电极部分的保护层，该电极不具有防止嵌入剂分子氧化/还原的附着探针。

公开(公告)号：US5985568A

公开(公告)日：1999-11-16

申请号：US923837

申请日：1997-09-04

申请人： Michael Krihak ,  Chan-Long Shieh

发明人： Michael Krihak ,  Chan-Long Shieh

IPC分类号： B01J19/00 ,  C40B40/06 ,  C40B60/14 ,  C12Q1/68 ,  C07H21/04 ,  C25D5/00

CPC分类号： C40B40/06 ,  B01J19/0046 ,  B82Y30/00 ,  C40B60/14 ,  B01J2219/00432 ,  B01J2219/00605 ,  B01J2219/00614 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/0072

摘要： A method of photoelectro-manipulation of target molecules including providing a photoconductive layer of material having a first and a second electrically conductive contact on a first surface thereof and a probe on an opposed second surface. A solution containing target molecules is positioned in electrical contact with the probe. A positive potential is connected between the solution and the first electrically conductive contact and a negative potential is connected between the solution and the second electrically conductive contact. A beam of light is directed through a portion of the photoconductive layer of material to complete an electrical circuit between one of the first and the second electrically conductive contacts and the solution, whereby target molecules in the solution are attracted to or repelled from the probe which is coupled into the electrical circuit by the beam of light.

摘要翻译： 包括在其第一表面上提供具有第一和第二导电接触的材料的光电导层和在相对的第二表面上的探针的光电操作靶分子的方法。 含有靶分子的溶液定位成与探针电接触。 溶液和第一导电接触点之间连接有正电位，并且在溶液和第二导电触点之间连接负电位。 光束被引导通过材料的光电导层的一部分，以完成第一和第二导电触点之间的电路和溶液，由此溶液中的目标分子被吸引或从探针排斥， 通过光束耦合到电路中。

公开(公告)号：US5810989A

公开(公告)日：1998-09-22

申请号：US923814

申请日：1997-09-04

申请人： Michael Krihak ,  Chan-Long Shieh ,  Hsing-Chung Lee

发明人： Michael Krihak ,  Chan-Long Shieh ,  Hsing-Chung Lee

IPC分类号： B01J19/00 ,  C07B61/00 ,  C07H21/00 ,  C07K1/04 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  C25D5/00 ,  C25B3/00 ,  C25D5/02

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  C07H21/00 ,  C07K1/045 ,  C07K1/047 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00614 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00711 ,  B01J2219/00713 ,  B01J2219/00722 ,  C07B2200/11 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14

摘要： A method of photoelectro-synthesizing probe arrays including the steps of providing a photoconductive layer of material having a layer of electrically conductive material on a first surface thereof and a solution of a plurality of a first oligonucleotide modified monomer positioned in electrical contact with an opposing second surface thereof such that a potential is connected therebetween. A beam of light is directed through a portion of the photoconductive layer of material to complete an electrical circuit between the layer of electrically conductive material and the solution through the portion of the photoconductive layer, whereby the monomers in the solution are electropolymerized on a surface area which is coupled into the electrical circuit by the beam of light.

摘要翻译： 一种光电合成探针阵列的方法，包括以下步骤：在其第一表面上提供具有导电材料层的材料的光电导层和多个第一寡核苷酸改性单体的溶液，所述第一寡核苷酸改性单体与相对的第二 表面，使得它们之间连接有电位。 光束被引导通过材料的光电导层的一部分，以完成在导电材料层与溶液之间的光电导层的部分之间的电路，由此溶液中的单体在表面积上电聚合 其通过光束耦合到电路中。

公开(公告)号：US07829272B2

公开(公告)日：2010-11-09

申请号：US11753533

申请日：2007-05-24

申请人： Eugene Tu ,  Donald E. Ackley ,  Anita Forster ,  Michael Krihak

发明人： Eugene Tu ,  Donald E. Ackley ,  Anita Forster ,  Michael Krihak

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12Q1/02

CPC分类号： A61K31/711 ,  Y10T428/2984

摘要： A method of generating pathogen detecting liposomes includes a step of providing molecular beacons with fluorescing components. The molecular beacons include either strands of RNA or DNA and the fluorescing components include an emitter and a quencher. The method further uses nanodroplet technology to encapsulate the molecular beacons within a lipid membrane. Subsequently, receptors are assembled in association with the membrane.

摘要翻译： 产生病原体检测脂质体的方法包括向分子信标提供荧光成分的步骤。 分子信标包括RNA或DNA的链，荧光成分包括发射体和猝灭剂。 该方法进一步使用纳米微滴技术将分子信标封装在脂质膜内。 随后，受体与膜结合而组装。

公开(公告)号：US20110033840A1

公开(公告)日：2011-02-10

申请号：US12906475

申请日：2010-10-18

申请人： Eugene Tu ,  Donald E. Ackley ,  Anita Forster ,  Michael Krihak

发明人： Eugene Tu ,  Donald E. Ackley ,  Anita Forster ,  Michael Krihak

IPC分类号： C12Q1/70 ,  C07H21/04

CPC分类号： A61K31/711 ,  Y10T428/2984

摘要： A method of generating pathogen detecting liposomes includes a step of providing molecular beacons with fluorescing components. The molecular beacons include either strands of RNA or DNA and the fluorescing components include an emitter and a quencher. The method further uses nanodroplet technology to encapsulate the molecular beacons within a lipid membrane. Subsequently, receptors are assembled in association with the membrane.

摘要翻译： 产生病原体检测脂质体的方法包括向分子信标提供荧光成分的步骤。 分子信标包括RNA或DNA的链，荧光成分包括发射体和猝灭剂。 该方法进一步使用纳米微滴技术将分子信标封装在脂质膜内。 随后，受体与膜结合而组装。

公开(公告)号：US20090148828A1

公开(公告)日：2009-06-11

申请号：US11753533

申请日：2007-05-24

申请人： Eugene Tu ,  Donald E. Ackley ,  Anita Forster ,  Michael Krihak

发明人： Eugene Tu ,  Donald E. Ackley ,  Anita Forster ,  Michael Krihak

IPC分类号： C12Q1/70

CPC分类号： A61K31/711 ,  Y10T428/2984

摘要： A method of generating pathogen detecting liposomes includes a step of providing molecular beacons with fluorescing components. The molecular beacons include either strands of RNA or DNA and the fluorescing components include an emitter and a quencher. The method further uses nanodroplet technology to encapsulate the molecular beacons within a lipid membrane. Subsequently, receptors are assembled in association with the membrane.

摘要翻译： 产生病原体检测脂质体的方法包括向分子信标提供荧光成分的步骤。 分子信标包括RNA或DNA的两条链，荧光成分包括发射体和猝灭剂。 该方法进一步使用纳米微滴技术将分子信标封装在脂质膜内。 随后，受体与膜结合而组装。