公开(公告)号：US20100006439A1

公开(公告)日：2010-01-14

申请号：US12559895

申请日：2009-09-15

申请人： Donhee Ham ,  Robert Westervelt ,  Thomas Hunt ,  Yong Liu ,  Hakho Lee

发明人： Donhee Ham ,  Robert Westervelt ,  Thomas Hunt ,  Yong Liu ,  Hakho Lee

IPC分类号： B03C5/02

CPC分类号： G01R33/302 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/043 ,  G01N15/1031 ,  G01N24/08 ,  G01N24/10 ,  G01R33/307 ,  G01R33/34092 ,  G01R33/3415 ,  G01R33/465 ,  G01R33/4808 ,  G01R33/5604

摘要： Methods and apparatus for manipulation, detection, imaging, characterization, sorting and/or assembly of biological or other materials, involving an integration of CMOS or other semiconductor-based technology and microfluidics. In one implementation, various components relating to the generation of electric and/or magnetic fields are implemented on an IC chip that is fabricated using standard protocols. The generated electric and/or magnetic fields are used to manipulate and/or detect one or more dielectric and/or magnetic particles and distinguish different types of particles. A microfluidic system is fabricated either directly on top of the IC chip, or as a separate entity that is then appropriately bonded to the IC chip, to facilitate the introduction and removal of cells in a biocompatible environment, or other particles/objects of interest suspended in a fluid. The patterned electric and/or magnetic fields generated by the IC chip can trap and move biological cells or other objects inside the microfluidic system. Electric and/or magnetic field generating components also may be controlled using signals of various frequencies so as to detect one or more cells, particles or objects of interest, and even the type of particle or object of interest, by measuring resonance characteristics associated with interactions between samples and one or more of the field-generating devices. Such systems may be employed in a variety of biological and medical related applications, including cell sorting and tissue assembly.

摘要翻译： 用于操作，检测，成像，表征，分选和/或组装生物或其他材料的方法和装置，涉及CMOS或其他基于半导体的技术和微流体的集成。 在一个实现中，与使用标准协议制造的IC芯片上实现与电场和/或磁场的产生有关的各种组件。 产生的电场和/或磁场用于操纵和/或检测一个或多个电介质和/或磁性颗粒并区分不同类型的颗粒。 微流体系统直接制造在IC芯片的顶部上，或者作为独立的实体，然后适当地结合到IC芯片，以便于在生物相容性环境或其他感兴趣的其它颗粒/物体中引入和去除细胞悬浮 在流体中。 由IC芯片产生的图案化电场和/或磁场可以捕获并移动微流体系统内的生物细胞或其它物体。 可以使用各种频率的信号来控制电场和/或磁场产生组件，以便通过测量与相互作用相关联的共振特性来检测一个或多个感兴趣的细胞，颗粒或感兴趣的物体，甚至是感兴趣的粒子或物体的类型 样本和一个或多个场产生装置之间。 这样的系统可以用于各种生物和医学相关应用，包括细胞分选和组织组装。

公开(公告)号：US20090168162A1

公开(公告)日：2009-07-02

申请号：US12087532

申请日：2007-01-12

申请人： Donhee Ham ,  Robert Westervelt ,  Thomas Hunt ,  Yong Liu ,  Hakho Lee

发明人： Donhee Ham ,  Robert Westervelt ,  Thomas Hunt ,  Yong Liu ,  Hakho Lee

IPC分类号： G02B21/26 ,  G02B21/32

CPC分类号： G02B21/34 ,  B01J2219/00653 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/043 ,  G01N15/1463 ,  G02B21/32

摘要： Microscopy methods and apparatus for manipulation, detection, imaging, characterization, sorting and/or assembly of biological or other materials, involving an integration of CMOS or other semiconductor-based technology and microfluidics in connection with a microscope. In one implementation, a microscope including optics and a stage is outfitted with various components relating to the generation of electric and/or magnetic fields, which are implemented on an IC chip. A microfluidic system is fabricated either directly on top of the IC chip, or as a separate entity that is then appropriately bonded to the IC chip, to facilitate the introduction and removal of cells in a biocompatible environment, or other particles/objects of interest suspended in a fluid. The patterned electric and/or magnetic fields generated by the IC chip can trap and move biological cells or other objects inside the microfluidic system to facilitate viewing via the microscope.

摘要翻译： 显微镜方法和用于操纵，检测，成像，表征，分选和/或组装生物或其他材料的装置，涉及CMOS或其他基于半导体的技术和与显微镜相结合的微流体的集成。 在一个实施方式中，包括光学器件和阶段的显微镜配备有与在IC芯片上实现的电场和/或磁场的产生相关的各种组件。 微流体系统直接制造在IC芯片的顶部上，或者作为独立的实体，然后适当地结合到IC芯片，以便于在生物相容性环境或其他感兴趣的其它颗粒/物体中引入和去除细胞悬浮 在流体中。 由IC芯片产生的图案化电场和/或磁场可捕获和移动微流体系统内的生物细胞或其它物体，以便于通过显微镜观察。

公开(公告)号：US20060020371A1

公开(公告)日：2006-01-26

申请号：US11105322

申请日：2005-04-13

申请人： Donhee Ham ,  Robert Westervelt ,  Thomas Hunt ,  Yong Liu ,  Hakho Lee

发明人： Donhee Ham ,  Robert Westervelt ,  Thomas Hunt ,  Yong Liu ,  Hakho Lee

IPC分类号： G05B99/00

CPC分类号： G01R33/302 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/043 ,  G01N15/1031 ,  G01N24/08 ,  G01N24/10 ,  G01R33/307 ,  G01R33/34092 ,  G01R33/3415 ,  G01R33/465 ,  G01R33/4808 ,  G01R33/5604

摘要： Methods and apparatus for manipulation, detection, imaging, characterization, sorting and/or assembly of biological or other materials, involving an integration of CMOS or other semiconductor-based technology and microfluidics. In one implementation, various components relating to the generation of electric and/or magnetic fields are implemented on an IC chip that is fabricated using standard protocols. The generated electric and/or magnetic fields are used to manipulate and/or detect one or more dielectric and/or magnetic particles and distinguish different types of particles. A microfluidic system is fabricated either directly on top of the IC chip, or as a separate entity that is then appropriately bonded to the IC chip, to facilitate the introduction and removal of cells in a biocompatible environment, or other particles/objects of interest suspended in a fluid. The patterned electric and/or magnetic fields generated by the IC chip can trap and move biological cells or other objects inside the microfluidic system. Electric and/or magnetic field generating components also may be controlled using signals of various frequencies so as to detect one or more cells, particles or objects of interest, and even the type of particle or object of interest, by measuring resonance characteristics associated with interactions between samples and one or more of the field-generating devices. Such systems may be employed in a variety of biological and medical related applications, including cell sorting and tissue assembly.

摘要翻译： 用于操作，检测，成像，表征，分选和/或组装生物或其他材料的方法和装置，涉及CMOS或其他基于半导体的技术和微流体的集成。 在一个实现中，与使用标准协议制造的IC芯片上实现与电场和/或磁场的产生有关的各种组件。 产生的电场和/或磁场用于操纵和/或检测一个或多个电介质和/或磁性颗粒并区分不同类型的颗粒。 微流体系统直接制造在IC芯片的顶部上，或者作为独立的实体，然后适当地结合到IC芯片，以便于在生物相容性环境或其他感兴趣的其它颗粒/物体中引入和去除细胞悬浮 在流体中。 由IC芯片产生的图案化电场和/或磁场可以捕获并移动微流体系统内的生物细胞或其它物体。 可以使用各种频率的信号来控制电场和/或磁场产生组件，以便通过测量与相互作用相关联的共振特性来检测一个或多个感兴趣的细胞，颗粒或感兴趣的物体，甚至是感兴趣的粒子或物体的类型 样本和一个或多个场产生装置之间。 这样的系统可以用于各种生物和医学相关应用，包括细胞分选和组织组装。

公开(公告)号：US20090220968A1

公开(公告)日：2009-09-03

申请号：US12224961

申请日：2007-03-09

申请人： David Issadore ,  Thomas Hunt ,  Kristi Adamson ,  Robert Westervelt ,  Rick Rogers

发明人： David Issadore ,  Thomas Hunt ,  Kristi Adamson ,  Robert Westervelt ,  Rick Rogers

IPC分类号： G01N33/53 ,  H05B6/80 ,  H05B6/72 ,  C12Q1/68 ,  C12M1/34

CPC分类号： H05B6/72 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/0822 ,  B01L2300/1866 ,  G01N1/44

摘要： Irradiation methods and apparatus configured to deliver power, via electromagnetic fields at a variety of frequencies and power levels, in a localized fashion to a target area. In one example, an electromagnetic field generator is disposed on a substrate and configured to deliver power via electromagnetic energy to a thin region proximate to (above) a surface of the substrate, wherein electromagnetic field intensity decreases significantly beyond the thin region. Such methods and apparatus are particularly useful in a wide variety of processes involving chemical and/or physical interactions in connection with a sample of interest located in the thin region. In different aspects, irradiator apparatus may be configured as disposable devices, and/or used in combination with one or more microfluidic or sensing components, for a variety of medical/laboratory/diagnostic methods and instrumentation implementations.

摘要翻译： 该照射方法和装置被配置成以局部方式通过各种频率和功率水平的电磁场向目标区域传递电力。 在一个示例中，电磁场发生器设置在基板上并且被配置为经由电磁能传递电力到靠近（高于）基板表面的薄区域，其中电磁场强度显着地减小到薄区域之外。 这种方法和装置在涉及与位于薄区域中的感兴趣样品相关的化学和/或物理相互作用的各种各样的方法中特别有用。 在不同方面，辐射器装置可被配置为一次性装置，和/或与一个或多个微流体或感测部件组合使用，用于各种医疗/实验室/诊断方法和仪器实施。

公开(公告)号：US20100255556A1

公开(公告)日：2010-10-07

申请号：US12602586

申请日：2008-06-26

申请人： Thomas Hunt ,  David Issadore ,  Robert Westervelt

发明人： Thomas Hunt ,  David Issadore ,  Robert Westervelt

IPC分类号： C12N13/00 ,  C12M1/42

CPC分类号： B01L3/502792 ,  B01L3/502761 ,  B01L2200/0626 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/043

摘要： The present disclosure relates generally to methods and apparatus for manipulating, detecting, imaging, and/or identifying particles, fluids, or other objects via electromagnetic fields, including methods and apparatus for identifying, sorting, splitting, coalescing, and/or reacting such particles, fluids, or other objects. Certain aspects of the invention are generally directe to methods and devices for producing electric or magnetic fields, e.g., from one or more field-generating components (200) (for example, arranged in an array), to control or manipulate a particle, fluid, or other object. For example, a fluidic droplet may be identified, sorted, separated, split, fused or coalesced, mixed, charged, sensed, determined, etc., using various systems and methods as described herein. In some cases, a particle, a fluidic species (e.g., a droplet), or another object may be contained or constrained by one or more layers of fluid. Other aspects of the invention are directed to methods of making such devices, methods of promoting the making or use of such devices, or the like.

摘要翻译： 本公开总体上涉及用于通过电磁场操纵，检测，成像和/或识别颗粒，流体或其它物体的方法和装置，包括用于识别，分选，聚结和/或反应这种颗粒的方法和装置 ，流体或其他物体。 本发明的某些方面通常涉及用于产生电场或磁场的方法和装置，例如，来自一个或多个场产生部件（200）（例如，排列成阵列），以控制或操纵颗粒，流体 ，或其他对象。 例如，使用本文所述的各种系统和方法，可以识别，分类，分离，分离，融合或聚结，混合，充电，感测，确定等流体液滴。 在一些情况下，颗粒，流体物质（例如，液滴）或另一物体可以被一层或多层流体包含或约束。 本发明的其它方面涉及制造这种装置的方法，促进制造或使用这种装置的方法等。

公开(公告)号：US20090219647A1

公开(公告)日：2009-09-03

申请号：US12085193

申请日：2006-11-06

申请人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski

发明人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski

IPC分类号： G11B5/58 ,  B01D57/02

CPC分类号： B03C5/005 ,  B03C5/026 ,  B82B3/00 ,  C12N13/00 ,  G01Q80/00 ,  G11B9/14 ,  G11B9/1409 ,  G11B11/002

摘要： Dielectrophoretic (DEP) tweezers apparatus and methods for various applications, including particle trapping. Two electrodes are disposed on or otherwise constitute an elongated object forming a tip. A voltage is applied across these electrodes to produce a non-uniform electromagnetic field proximate to the tip thereby creating a dielectrophoretic trap. Once trapped, a particle may be moved to desired locations via manipulation of the elongated object or the medium in which the particle is located. Multiple DEP tweezers apparatus may be arranged to form arrays of tips capable of respectively generating local electromagnetic fields confined to the tips. Such DEP arrays may be employed in nanofabrication processes involving nanolithography or nano-manipulation, as well as data storage and retrieval applications.

摘要翻译： 介电泳（DEP）镊子用于各种应用的设备和方法，包括颗粒捕获。 两个电极设置在或以其他方式构成形成尖端的细长物体。 在这些电极之间施加电压以在尖端附近产生不均匀的电磁场，从而产生介电电泳阱。 一旦被捕获，颗粒可以通过操纵细长物体或颗粒所在的介质而移动到所需位置。 多个DEP镊子装置可以被布置成形成能够分别产生局限于尖端的局部电磁场的尖端阵列。 这种DEP阵列可以用于涉及纳米光刻或纳米操作的纳米制造工艺，以及数据存储和检索应用。

公开(公告)号：US08262885B2

公开(公告)日：2012-09-11

申请号：US12085193

申请日：2006-11-06

申请人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski

发明人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski

IPC分类号： B01D57/02

CPC分类号： B03C5/005 ,  B03C5/026 ,  B82B3/00 ,  C12N13/00 ,  G01Q80/00 ,  G11B9/14 ,  G11B9/1409 ,  G11B11/002

摘要： Dielectrophoretic (DEP) tweezers apparatus and methods for various applications, including particle trapping. Two electrodes are disposed on or otherwise constitute an elongated object forming a tip. A voltage is applied across these electrodes to produce a non-uniform electromagnetic field proximate to the tip thereby creating a dielectrophoretic trap. Once trapped, a particle may be moved to desired locations via manipulation of the elongated object or the medium in which the particle is located. Multiple DEP tweezers apparatus may be arranged to form arrays of tips capable of respectively generating local electromagnetic fields confined to the tips. Such DEP arrays may be employed in nanofabrication processes involving nanolithography or nano-manipulation, as well as data storage and retrieval applications.

摘要翻译： 介电泳（DEP）镊子用于各种应用的设备和方法，包括颗粒捕获。 两个电极设置在或以其他方式构成形成尖端的细长物体。 在这些电极之间施加电压以在尖端附近产生不均匀的电磁场，从而产生介电电泳阱。 一旦被捕获，颗粒可以通过操纵细长物体或颗粒所在的介质而移动到所需位置。 多个DEP镊子装置可以被布置成形成能够分别产生局限于尖端的局部电磁场的尖端阵列。 这种DEP阵列可以用于涉及纳米光刻或纳米操作的纳米制造工艺，以及数据存储和检索应用。

公开(公告)号：US08021532B2

公开(公告)日：2011-09-20

申请号：US12388660

申请日：2009-02-19

申请人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski ,  Jonathan Aguilar ,  Keith Brown

发明人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski ,  Jonathan Aguilar ,  Keith Brown

IPC分类号： B03C5/02

CPC分类号： B03C5/005 ,  B03C5/026 ,  G01Q80/00

摘要： Dielectrophoretic (DEP) tweezers apparatus and methods for various applications, including particle trapping. Multiple electrodes (e.g., two or three) are disposed on or otherwise constitute an elongated object forming a tip. Exemplary electrode configurations include, but are not limited to, coaxial and triaxial arrangements. A voltage is applied across these electrodes to produce a non-uniform electromagnetic field proximate to the tip thereby creating a dielectrophoretic trap. Once trapped, a particle may be moved to desired locations via manipulation of the elongated object or the medium in which the particle is located. Multiple DEP tweezers apparatus may be arranged to form arrays of tips capable of respectively generating local electromagnetic fields confined to the tips. Such DEP arrays may be employed in nanofabrication processes involving nanolithography or nano-manipulation, as well as data storage and retrieval applications.

摘要翻译： 介电泳（DEP）镊子用于各种应用的设备和方法，包括颗粒捕获。 多个电极（例如，两个或三个）设置在或以其他方式构成形成尖端的细长物体。 示例性电极配置包括但不限于同轴和三轴布置。 在这些电极之间施加电压以在尖端附近产生不均匀的电磁场，从而产生介电电泳阱。 一旦被捕获，颗粒可以通过操纵细长物体或颗粒所在的介质而移动到所需位置。 多个DEP镊子装置可以被布置成形成能够分别产生局限于尖端的局部电磁场的尖端阵列。 这种DEP阵列可以用于涉及纳米光刻或纳米操作的纳米制造工艺，以及数据存储和检索应用。

公开(公告)号：US20090211910A1

公开(公告)日：2009-08-27

申请号：US12388660

申请日：2009-02-19

申请人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski ,  Jonathan Aguilar ,  Keith Brown

发明人： Thomas Hunt ,  Robert Westervelt ,  Ania Bleszynski ,  Jonathan Aguilar ,  Keith Brown

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： B03C5/005 ,  B03C5/026 ,  G01Q80/00

摘要： Dielectrophoretic (DEP) tweezers apparatus and methods for various applications, including particle trapping. Multiple electrodes (e.g., two or three) are disposed on or otherwise constitute an elongated object forming a tip. Exemplary electrode configurations include, but are not limited to, coaxial and triaxial arrangements. A voltage is applied across these electrodes to produce a non-uniform electromagnetic field proximate to the tip thereby creating a dielectrophoretic trap. Once trapped, a particle may be moved to desired locations via manipulation of the elongated object or the medium in which the particle is located. Multiple DEP tweezers apparatus may be arranged to form arrays of tips capable of respectively generating local electromagnetic fields confined to the tips. Such DEP arrays may be employed in nanofabrication processes involving nanolithography or nano-manipulation, as well as data storage and retrieval applications.

摘要翻译： 介电泳（DEP）镊子用于各种应用的设备和方法，包括颗粒捕获。 多个电极（例如，两个或三个）设置在或以其他方式构成形成尖端的细长物体。 示例性电极配置包括但不限于同轴和三轴布置。 在这些电极之间施加电压以在尖端附近产生不均匀的电磁场，从而产生介电电泳阱。 一旦被捕获，颗粒可以通过操纵细长物体或颗粒所在的介质而移动到所需位置。 多个DEP镊子装置可以被布置成形成能够分别产生局限于尖端的局部电磁场的尖端阵列。 这种DEP阵列可以用于涉及纳米光刻或纳米操作的纳米制造工艺，以及数据存储和检索应用。

公开(公告)号：US20080007752A1

公开(公告)日：2008-01-10

申请号：US11789206

申请日：2007-04-24

申请人： Lakhbir Gandhi ,  Robert Westervelt ,  Kenneth Schmidt ,  Dongpei Su ,  Alfred Abkarian ,  Mark Raley

发明人： Lakhbir Gandhi ,  Robert Westervelt ,  Kenneth Schmidt ,  Dongpei Su ,  Alfred Abkarian ,  Mark Raley

IPC分类号： G06F15/00

CPC分类号： H04N1/58

摘要： A system and method for trapping in electrophotographic color printing and related technologies for printing or display in which the final image is an overlay of multiple components subject to alignment errors. Trapping is based on the cyan (C), magenta (M), and black (K) planes. There are four steps as follows: detect object edges on each of the four color planes; detect coincident and opposing edge transitions on each pair of planes (CM, CK, and KM); determine which plane to trap, i.e., to extend object across edge; and generate trap on that plane using a simple trap generation rule and a single trap generation rule.

摘要翻译： 用于捕获电子照相彩色打印的系统和方法以及用于打印或显示的相关技术，其中最终图像是受到对准误差的多个部件的覆盖。 捕获是基于青色（C），品红色（M）和黑色（K）面。 有四个步骤如下：检测四个颜色平面中的每一个上的物体边缘; 检测每对平面（CM，CK和KM）上的重合和相对的边缘过渡; 确定要捕获的平面，即，跨越边缘延伸物体; 并使用简单的陷阱生成规则和单个陷阱生成规则在该平面上生成陷阱。