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    Method for identifying genes encoding secreted or membrane-associated proteins
    1.
    发明授权
    Method for identifying genes encoding secreted or membrane-associated proteins 失效
    用于鉴定编码分泌或膜相关蛋白的基因的方法

    公开(公告)号：US5952171A

    公开(公告)日：1999-09-14

    申请号：US752307

    申请日：1996-11-19

    申请人： Sean Anthony McCarthy ,  David Paul Gearing ,  Douglas Adam Levinson

    发明人： Sean Anthony McCarthy ,  David Paul Gearing ,  Douglas Adam Levinson

    IPC分类号： C12N15/10 ,  C12N15/85 ,  C12Q1/68

    CPC分类号： C12Q1/6811 ,  C12N15/1034 ,  C12N15/1051 ,  C12N15/85 ,  C12N2800/60 ,  C12N2830/38

    摘要： The invention features a method for identifying a cDNA nucleic acid encoding a mammalian protein having a signal sequence, which method includes the following steps: (a) providing library of mammalian cDNA; (b) ligating the library of mammalian cDNA to DNA encoding alkaline phosphatase lacking both a signal sequence and a membrane anchor sequence to form ligated DNA; (c) transforming bacterial cells with the ligated DNA to create a bacterial cell clone library; (d) isolating DNA comprising the mammalian cDNA from at least one clone in the bacterial cell clone library; (e) separately transfecting DNA isolated from clones in step (d) into mammalian cells which do not express alkaline phosphatase to create a mammalian cell clone library wherein each clone in the mammalian cell clone library corresponds to a clone in the bacterial cell clone library; (f) identifying a clone in the mammalian cell clone library which express alkaline phosphatase; (g) identifying the clone in the bacterial cell clone library corresponding to the clone in the mammalian cell clone library identified in step (f); and (h) isolating and sequencing a portion of the mammalian cDNA present in the bacterial cell library clone identified in step (g) to identify a mammalian cDNA encoding a mammalian protein having a signal sequence.

    摘要翻译： 本发明的特征在于鉴定编码具有信号序列的哺乳动物蛋白质的cDNA核酸的方法，该方法包括以下步骤：（a）提供哺乳动物cDNA文库; （b）将哺乳动物cDNA文库与编码缺乏信号序列和膜锚定序列的碱性磷酸酶的DNA连接以形成连接的DNA; （c）用连接的DNA转化细菌细胞以产生细菌细胞克隆文库; （d）从细菌细胞克隆文库中的至少一个克隆分离包含哺乳动物cDNA的DNA; （e）将步骤（d）中从克隆分离的DNA分别转染到不表达碱性磷酸酶的哺乳动物细胞中以产生哺乳动物细胞克隆文库，其中哺乳动物细胞克隆文库中的每个克隆对应于细菌细胞克隆文库中的克隆; （f）鉴定表达碱性磷酸酶的哺乳动物细胞克隆文库中的克隆; （g）鉴定对应于步骤（f）中鉴定的哺乳动物细胞克隆文库中克隆的细菌细胞克隆文库中的克隆; 和（h）分离和测序存在于步骤（g）中鉴定的细菌细胞库克隆中的哺乳动物cDNA的一部分，以鉴定编码具有信号序列的哺乳动物蛋白质的哺乳动物cDNA。

    Compositions and methods for the treatment and diagnosis of immune disorders
    2.
    发明申请
    Compositions and methods for the treatment and diagnosis of immune disorders 审中-公开
    用于治疗和诊断免疫疾病的组合物和方法

    公开(公告)号：US20090041752A1

    公开(公告)日：2009-02-12

    申请号：US11888354

    申请日：2007-07-31

    申请人： Douglas Adam Levinson ,  Clare M. Lloyd ,  Sean A. McCarthy

    发明人： Douglas Adam Levinson ,  Clare M. Lloyd ,  Sean A. McCarthy

    IPC分类号： A61K39/395 ,  A61K38/00 ,  A61P9/00

    CPC分类号： C07K14/47

    摘要： The present invention relates to methods and compositions for the treatment and diagnosis of immune disorders, especially T helper lymphocyte-related disorders, and also for the treatment of mast cell-related processes and disorders, ischemic disorders and injuries, including ischemic renal disorders and injuries. For example, genes which are differentially expressed within and among T helper (TH) cells and TH cell subpopulations, which include, but are not limited to TH0, TH1 and TH2 cell subpopulations are identified. Genes are also identified via the ability of their gene products to interact with gene products involved in the differentiation, maintenance and effector function of such TH cells and TH cell subpopulations. The genes identified can be used diagnostically or as targets for therapeutic intervention. In this regard, the present invention provides methods for the identification and therapeutic use of compounds as treatments of immune disorders, especially TH cell subpopulation-related disorders. Additionally, methods are provided for the diagnostic evaluation and prognosis of TH cell subpopulation-related disorders, for the identification of subjects exhibiting a predisposition to such conditions, for monitoring patients undergoing clinical evaluation for the treatment of such disorders, and for monitoring the efficacy of compounds used in clinical trials. Methods are also provided for the treatment of symptoms associated with mast cell-related processes or disorders and ischemic disorders and injuries using the genes, gene products and antibodies of the invention.

    摘要翻译： 本发明涉及用于治疗和诊断免疫疾病，特别是T辅助淋巴细胞相关病症的方法和组合物，还涉及治疗肥大细胞相关过程和病症，缺血性疾病和损伤，包括缺血性肾病和损伤 。 例如，识别在T辅助细胞（TH）细胞和TH细胞亚群内部和之间差异表达的基因，其包括但不限于TH0，TH1和TH2细胞亚群。 基因也通过其基因产物与涉及TH细胞和TH细胞亚群的分化，维持和效应子功能的基因产物相互作用的能力进行鉴定。 所鉴定的基因可以诊断使用或作为治疗干预的靶标。 在这方面，本发明提供了用于鉴定和治疗性使用化合物作为免疫疾病，特别是TH细胞亚群相关病症治疗的方法。 另外，提供了用于TH细胞亚群相关病症的诊断评估和预后的方法，用于鉴定表现出对这些病症倾向的受试者，用于监测接受临床评估治疗这种病症的患者，以及用于监测 化合物用于临床试验。 还提供了使用本发明的基因，基因产物和抗体治疗与肥大细胞相关过程或病症相关的症状以及缺血性疾病和损伤的方法。

    COMPOSITIONS AND METHODS FOR THE TREATMENT AND DIAGNOSIS OF IMMUNE DISORDERS
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR THE TREATMENT AND DIAGNOSIS OF IMMUNE DISORDERS 审中-公开
    用于治疗和诊断免疫缺陷病的组合物和方法

    公开(公告)号：US20110123548A1

    公开(公告)日：2011-05-26

    申请号：US12729438

    申请日：2010-03-23

    申请人： Douglas Adam Levinson ,  Clare M. Lloyd ,  Sean A. McCarthy

    发明人： Douglas Adam Levinson ,  Clare M. Lloyd ,  Sean A. McCarthy

    IPC分类号： A61K39/395 ,  A61K38/17 ,  A61K48/00 ,  A61P9/10 ,  A61P13/12

    CPC分类号： C07K14/47

    摘要： The present invention relates to methods and compositions for the treatment and diagnosis of immune disorders, especially T helper lymphocyte-related disorders, and also for the treatment of mast cell-related processes and disorders, ischemic disorders and injuries, including ischemic renal disorders and injuries. For example, genes which are differentially expressed within and among T helper (TH) cells and TH cell subpopulations, which include, but are not limited to TH0, TH1 and TH2 cell subpopulations are identified. Genes are also identified via the ability of their gene products to interact with gene products involved in the differentiation, maintenance and effector function of such TH cells and TH cell subpopulations. The genes identified can be used diagnostically or as targets for therapeutic intervention. In this regard, the present invention provides methods for the identification and therapeutic use of compounds as treatments of immune disorders, especially TH cell subpopulation-related disorders. Additionally, methods are provided for the diagnostic evaluation and prognosis of TH cell subpopulation-related disorders, for the identification of subjects exhibiting a predisposition to such conditions, for monitoring patients undergoing clinical evaluation for the treatment of such disorders, and for monitoring the efficacy of compounds used in clinical trials. Methods are also provided for the treatment of symptoms associated with mast cell-related processes or disorders and ischemic disorders and injuries using the genes, gene products and antibodies of the invention.

    摘要翻译： 本发明涉及用于治疗和诊断免疫疾病，特别是T辅助淋巴细胞相关病症的方法和组合物，还涉及治疗肥大细胞相关过程和病症，缺血性疾病和损伤，包括缺血性肾病和损伤 。 例如，识别在T辅助细胞（TH）细胞和TH细胞亚群内部和之间差异表达的基因，其包括但不限于TH0，TH1和TH2细胞亚群。 基因也通过其基因产物与涉及TH细胞和TH细胞亚群的分化，维持和效应子功能的基因产物相互作用的能力进行鉴定。 所鉴定的基因可以诊断使用或作为治疗干预的靶标。 在这方面，本发明提供了用于鉴定和治疗性使用化合物作为免疫疾病，特别是TH细胞亚群相关病症治疗的方法。 另外，提供了用于TH细胞亚群相关病症的诊断评估和预后的方法，用于鉴定表现出对这些病症倾向的受试者，用于监测接受临床评估治疗这种病症的患者，以及用于监测 化合物用于临床试验。 还提供了使用本发明的基因，基因产物和抗体治疗与肥大细胞相关过程或病症相关的症状以及缺血性疾病和损伤的方法。

    Compositions and methods for the treatment and diagnosis of immune disorders
    6.
    发明授权
    Compositions and methods for the treatment and diagnosis of immune disorders 有权
    用于治疗和诊断免疫疾病的组合物和方法

    公开(公告)号：US07172750B2

    公开(公告)日：2007-02-06

    申请号：US10252131

    申请日：2002-09-20

    申请人： Douglas Adam Levinson

    发明人： Douglas Adam Levinson

    IPC分类号： A61K49/00 ,  A61K39/395 ,  A61K39/40 ,  C07H21/04

    CPC分类号： A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2227/50 ,  A01K2267/03 ,  A01K2267/0325 ,  A01K2267/0368 ,  A01K2267/0381 ,  A01K2267/0387 ,  A61K38/00 ,  B60G13/003 ,  C07K14/70503 ,  C07K16/2803 ,  C07K19/00 ,  C07K2319/00 ,  C12N9/6467 ,  C12N9/6472 ,  C12N15/8509 ,  C12N2800/30 ,  C12N2830/00 ,  C12Q1/6809 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S424/81 ,  Y10S530/868

    摘要： The present invention relates to methods and compositions for the treatment and diagnosis of immune disorders, especially T helper lymphocyte-related disorders. For example, genes which are differentially expressed within and among T helper (TH) cells and TH cell subpopulations, which include, but are not limited to TH0, TH1 and TH2 cell subpopulations are identified. Genes are also identified via the ability of their gene products to interact with gene products involved in the differentiation, maintenance and effector function of such TH cells and TH cell subpopulations. The genes identified can be used diagnostically or as targets for therapeutic intervention. In this regard, the present invention provides methods for the identification and therapeutic use of compounds as treatments of immune disorders, especially TH cell subpopulation-related disorders. Additionally, methods are provided for the diagnostic evaluation and prognosis of TH cell subpopulation-related disorders, for the identification of subjects exhibiting a predisposition to such conditions, for monitoring patients undergoing clinical evaluation for the treatment of such disorders, and for monitoring the efficacy of compounds used in clinical trials.

    摘要翻译： 本发明涉及用于治疗和诊断免疫疾病，特别是T辅助淋巴细胞相关疾病的方法和组合物。 例如，识别在T辅助细胞（TH）细胞和TH细胞亚群内部和之间差异表达的基因，其包括但不限于TH0，TH1和TH2细胞亚群。 基因也通过其基因产物与涉及TH细胞和TH细胞亚群的分化，维持和效应子功能的基因产物相互作用的能力进行鉴定。 所鉴定的基因可以诊断使用或作为治疗干预的靶标。 在这方面，本发明提供了用于鉴定和治疗性使用化合物作为免疫疾病，特别是TH细胞亚群相关病症治疗的方法。 另外，提供了用于TH细胞亚群相关病症的诊断评估和预后的方法，用于鉴定表现出对这些病症倾向的受试者，用于监测接受临床评估治疗这种病症的患者，以及用于监测 化合物用于临床试验。

    Secreted neural adhesion proteins
    7.
    发明授权
    Secreted neural adhesion proteins 失效
    分泌神经粘附蛋白

    公开(公告)号：US06395872B1

    公开(公告)日：2002-05-28

    申请号：US09991326

    申请日：2001-11-21

    申请人： Sean Anthony McCarthy ,  David Paul Gearing ,  Douglas Adam Levinson

    发明人： Sean Anthony McCarthy ,  David Paul Gearing ,  Douglas Adam Levinson

    IPC分类号： C07K700

    CPC分类号： C12Q1/6811 ,  C12N15/1034 ,  C12N15/1051 ,  C12N15/85 ,  C12N2800/60 ,  C12N2830/38

    摘要： The invention features a method for identifying a cDNA nucleic acid encoding a mammalian protein having a signal sequence, which method includes the following steps: (a) providing library of mammalian cDNA; (b) ligating the library of mammalian cDNA to DNA encoding alkaline phosphatase lacking both a signal sequence and a membrane anchor sequence to form ligated DNA; 8 transforming bacterial cells with the ligated DNA to create a bacterial cell clone library; (d) isolating DNA comprising the mammalian cDNA from at least one clone in the bacterial cell clone library; (e) separately transfecting DNA isolated from clones in step (d) into mammalian cells which do not express alkaline phosphatase to create a mammalian cell clone library wherein each clone in the mammalian cell clone library corresponds to a clone in the bacterial cell clone library; (f) identifying a clone in the mammalian cell clone library which expresses alkaline phosphatase; (g) identifying the clone in the bacterial cell clone library corresponding to the clone in the mammalian cell clone library identified in step (f); and (h) isolating and sequencing a portion of the mammalian cDNA present in the bacterial cell library clone identified in step (g) to identify a mammalian cDNA encoding a mammalian protein having a signal sequence.

    摘要翻译： 本发明的特征在于鉴定编码具有信号序列的哺乳动物蛋白质的cDNA核酸的方法，该方法包括以下步骤：（a）提供哺乳动物cDNA文库; （b）将哺乳动物cDNA文库与编码缺乏信号序列和膜锚定序列的碱性磷酸酶的DNA连接以形成连接的DNA; 8用连接的DNA转化细菌细胞以产生细菌细胞克隆文库; （d）从细菌细胞克隆文库中的至少一个克隆分离包含哺乳动物cDNA的DNA; （e）将步骤（d）中从克隆分离的DNA分别转染到不表达碱性磷酸酶的哺乳动物细胞中以产生哺乳动物细胞克隆文库，其中哺乳动物细胞克隆文库中的每个克隆对应于细菌细胞克隆文库中的克隆; （f）鉴定表达碱性磷酸酶的哺乳动物细胞克隆文库中的克隆; （g）鉴定对应于步骤（f）中鉴定的哺乳动物细胞克隆文库中克隆的细菌细胞克隆文库中的克隆; 和（h）分离和测序存在于步骤（g）中鉴定的细菌细胞库克隆中的哺乳动物cDNA的一部分，以鉴定编码具有信号序列的哺乳动物蛋白质的哺乳动物cDNA。