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公开(公告)号：US20080287311A1
公开(公告)日：2008-11-20
申请号：US11996496
申请日：2006-07-24
CPC分类号： C12N15/1075
摘要： A method is provided for selecting membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalised nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.
摘要翻译： 提供了从能够穿透或穿透脂质膜的肽展示文库中选择膜转位肽（MTP）的方法。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。
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公开(公告)号：US08267616B2
公开(公告)日：2012-09-18
申请号：US12308927
申请日：2007-07-11
申请人： Duncan McGregor
发明人： Duncan McGregor
IPC分类号： B25G3/20
CPC分类号： E05C3/043 , E05B3/00 , Y10T292/0961 , Y10T292/1084 , Y10T403/32606 , Y10T403/7051
摘要： A pivot joint includes a first component (12) having a projecting boss form (16) and a second component (11) having an opening (25) for receiving the boss form (16). A headed fastener (32) and washer (27) engage with the boss form (16), which includes engagement elements (17) that dig into the washer (27) when the joint is assembled.
摘要翻译： 枢转接头包括具有突出凸台形式（16）的第一部件（12）和具有用于接收凸台形式（16）的开口（25）的第二部件（11）。 头部紧固件（32）和垫圈（27）与凸台形式（16）接合，其包括当接头组装时挖入垫圈（27）的接合元件（17）。
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公开(公告)号：US07842476B2
公开(公告)日：2010-11-30
申请号：US10526479
申请日：2003-09-05
申请人： Duncan McGregor , Richard Odegrip , Kevin Fizgerald , Rosemarie Hederer , Bill Eldridge , Chris Ullman , Philip Kuhlman , David Coomber
发明人： Duncan McGregor , Richard Odegrip , Kevin Fizgerald , Rosemarie Hederer , Bill Eldridge , Chris Ullman , Philip Kuhlman , David Coomber
CPC分类号： C12N15/1075 , C07K2319/80
摘要： The invention provides a method for making in vitro peptide expression libraries, and for the isolation of nucleotide sequences encoding peptides of interest, wherein the peptides or proteins are specifically associated with the DNA encoding them through non-covalent protein:DNA binding. The method describes ways of making the library itself, DNA molecules encoding the library and uses of the expression library.
摘要翻译： 本发明提供了制备体外肽表达文库以及分离编码目的肽的核苷酸序列的方法，其中肽或蛋白质通过非共价蛋白质DNA结合与编码它们的DNA特异性结合。 该方法描述了制作库本身的方法，编码该库的DNA分子和表达库的用途。
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公开(公告)号：US08679781B2
公开(公告)日：2014-03-25
申请号：US12909420
申请日：2010-10-21
申请人： Duncan McGregor , Richard Odegrip , Kevin Fitzgerald , Rosemarie Hederer , Bill Eldridge , Chris Ullman , Philip Kuhlman , David Coomber
发明人： Duncan McGregor , Richard Odegrip , Kevin Fitzgerald , Rosemarie Hederer , Bill Eldridge , Chris Ullman , Philip Kuhlman , David Coomber
CPC分类号： C12N15/1075 , C07K2319/80
摘要： The invention provides a method for making in vitro peptide expression libraries, and for the isolation of nucleotide sequences encoding peptides of interest, wherein the peptides or proteins are specifically associated with the DNA encoding them through non-covalent protein:DNA binding. The method describes ways of making the library itself, DNA molecules encoding the library and uses of the expression library.
摘要翻译： 本发明提供了制备体外肽表达文库以及分离编码目的肽的核苷酸序列的方法，其中肽或蛋白质通过非共价蛋白质DNA结合与编码它们的DNA特异性结合。 该方法描述了制作库本身的方法，编码该库的DNA分子和表达库的用途。
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公开(公告)号：US20100047009A1
公开(公告)日：2010-02-25
申请号：US12308927
申请日：2007-07-11
申请人： Duncan McGregor
发明人： Duncan McGregor
CPC分类号： E05C3/043 , E05B3/00 , Y10T292/0961 , Y10T292/1084 , Y10T403/32606 , Y10T403/7051
摘要： A pivot joint includes a first component (12) having a projecting boss form (16) and a second component (11) having an opening (25) for receiving the boss form (16). A headed fastener (32) and washer (27) engage with the boss form (16), which includes engagement elements (17) that dig into the washer (27) when the joint is assembled.
摘要翻译： 枢转接头包括具有突出凸台形式（16）的第一部件（12）和具有用于接收凸台形式（16）的开口（25）的第二部件（11）。 头部紧固件（32）和垫圈（27）与凸台形式（16）接合，其包括当接头组装时挖入垫圈（27）的接合元件（17）。
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公开(公告)号：US07312074B1
公开(公告)日：2007-12-25
申请号：US09486882
申请日：1998-09-02
申请人： Duncan McGregor
发明人： Duncan McGregor
CPC分类号： C07K14/70567 , C07K2319/00 , C12N15/1075
摘要： There is described a method of isolating nucleotide sequences encoding target peptides from DNA libraries using DNA binding proteins to link the peptide to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest, or from synthetic DNA, and inserted into, or adjacent to, a DNA binding protein in an expression vector to create a chimeric fusion protein. Incorporation of the vector DNA into a carrier package, during expression of the chimeric fusion protein, results in the production of a peptide display carrier package (PDCP) displaying the DNA-bound fusion protein on the external surface of the carrier package. Employment of affinity purification techniques results in the PDCP particles containing sequences encoding the desired peptide to be selected and the desired nucleotide sequences obtained therefrom.
摘要翻译： 描述了使用DNA结合蛋白从DNA文库分离编码靶肽的核苷酸序列以将肽连接到编码它的序列的方法。 从编码目标蛋白的细胞或合成的DNA制备DNA文库，并将其插入到表达载体中的DNA结合蛋白中或与DNA结合蛋白相邻，以产生嵌合融合蛋白。 在嵌合融合蛋白的表达期间将载体DNA并入载体包中导致在载体包装的外表面上显示DNA结合融合蛋白的肽显示载体包装（PDCP）的制备。 使用亲和纯化技术导致含有编码要选择的所需肽的序列的PDCP颗粒和由其获得的所需核苷酸序列。
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公开(公告)号：US20140154241A1
公开(公告)日：2014-06-05
申请号：US13991178
申请日：2011-12-05
申请人： Duncan McGregor , William Eldridge , Simon Robins , Marie Fernie , Tricia White , Stuart Pritchard , Susan King
发明人： Duncan McGregor , William Eldridge , Simon Robins , Marie Fernie , Tricia White , Stuart Pritchard , Susan King
IPC分类号： C07K16/18
CPC分类号： C07K16/18 , C07K14/461 , C07K2317/20 , C07K2317/565 , C07K2319/31
摘要： A modified igNAR peptide sequence derived from a wild-type igNAR peptide sequence is diversified by mutating the amino acid sequence at 50% or more of the amino acids in the CDR3 loop region and optionally at 50% or more of the amino acids in the CDR3 loop region. The modified igNAR peptide may have the sequence of SEQ ID NO: 8, 10 or 50 to 85. The modified igNAR peptides have binding activity against albumin protein sequences, such as human serum albumin. These modified igNAR peptides may have utility in extending the in vivo half-life of biological molecules e.g. therapeutic agents, and so may be used in medicine.
摘要翻译： 衍生自野生型igNAR肽序列的修饰的igNAR肽序列通过在CDR3环区域中的任何氨基酸的50％或更多的氨基酸序列突变并且任选地在CDR3中的50％或更多个的氨基酸中变异 循环区域。 修饰的igNAR肽可以具有SEQ ID NO：8,10或50至85的序列。修饰的igNAR肽对白蛋白蛋白序列如人血清白蛋白具有结合活性。 这些修饰的igNAR肽可用于延长生物分子的体内半衰期，例如， 治疗剂等也可用于医药。
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公开(公告)号：US08735146B2
公开(公告)日：2014-05-27
申请号：US11985355
申请日：2007-11-13
申请人： Duncan McGregor
发明人： Duncan McGregor
CPC分类号： C07K14/70567 , C07K2319/00 , C12N15/1075
摘要： There is described a method of isolating nucleotide sequences encoding target peptides from DNA libraries using DNA binding proteins to link the peptide to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest, or from synthetic DNA, and inserted into, or adjacent to, a DNA binding protein in an expression vector to create a chimeric fusion protein. Incorporation of the vector DNA into a carrier package, during expression of the chimeric fusion protein, results in the production of a peptide display carrier package (PDCP) displaying the DNA-bound fusion protein on the external surface of the carrier package. Employment of affinity purification techniques results in the PDCP particles containing sequences encoding the desired peptide to be selected and the desired nucleotide sequences obtained therefrom.
摘要翻译： 描述了使用DNA结合蛋白从DNA文库分离编码靶肽的核苷酸序列以将肽连接到编码它的序列的方法。 从编码目标蛋白的细胞或合成的DNA制备DNA文库，并将其插入到表达载体中的DNA结合蛋白中或与DNA结合蛋白相邻，以产生嵌合融合蛋白。 在嵌合融合蛋白的表达期间将载体DNA并入载体包中导致在载体包装的外表面上显示DNA结合融合蛋白的肽显示载体包装（PDCP）的制备。 使用亲和纯化技术导致含有编码要选择的所需肽的序列的PDCP颗粒和由其获得的所需核苷酸序列。
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公开(公告)号：US08557744B2
公开(公告)日：2013-10-15
申请号：US11996496
申请日：2006-07-24
CPC分类号： C12N15/1075
摘要： A method is selects membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalized nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.
摘要翻译： 一种方法是从能够穿透或穿透脂质膜的肽展示文库中选择膜 - 位移肽（MTP）。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。
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公开(公告)号：US20080220981A1
公开(公告)日：2008-09-11
申请号：US11985355
申请日：2007-11-13
申请人： Duncan McGregor
发明人： Duncan McGregor
CPC分类号： C07K14/70567 , C07K2319/00 , C12N15/1075
摘要： There is described a method of isolating nucleotide sequences encoding target peptides from DNA libraries using DNA binding proteins to link the peptide to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest, or from synthetic DNA, and inserted into, or adjacent to, a DNA binding protein in an expression vector to create a chimeric fusion protein. Incorporation of the vector DNA into a carrier package, during expression of the chimeric fusion protein, results in the production of a peptide display carrier package (PDCP) displaying the DNA-bound fusion protein on the external surface of the carrier package. Employment of affinity purification techniques results in the PDCP particles containing sequences encoding the desired peptide to be selected and the desired nucleotide sequences obtained therefrom.
摘要翻译： 描述了使用DNA结合蛋白从DNA文库分离编码靶肽的核苷酸序列以将肽连接到编码它的序列的方法。 从编码目标蛋白的细胞或合成的DNA制备DNA文库，并将其插入到表达载体中的DNA结合蛋白中或与DNA结合蛋白相邻，以产生嵌合融合蛋白。 在嵌合融合蛋白的表达期间将载体DNA并入载体包中导致在载体包装的外表面上显示DNA结合融合蛋白的肽显示载体包装（PDCP）的制备。 使用亲和纯化技术导致含有编码要选择的所需肽的序列的PDCP颗粒和由其获得的所需核苷酸序列。
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