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    RECOMBINANT MICROBIAL CELLS THAT PRODUCE AT LEAST 28% EICOSAPENTAENOIC ACID AS DRY CELL WEIGHT
    2.
    发明申请
    RECOMBINANT MICROBIAL CELLS THAT PRODUCE AT LEAST 28% EICOSAPENTAENOIC ACID AS DRY CELL WEIGHT 有权
    产生至少28％的重要脂肪酸作为干细胞重量的重组微生物细胞

    公开(公告)号：US20140186906A1

    公开(公告)日：2014-07-03

    申请号：US14132022

    申请日：2013-12-18

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Quinn Qun Zhu ,  Seung-Pyo Hong ,  Pamela L. Sharpe ,  Narendra S. Yadav ,  Xiaochun Fan ,  Michael Dauner

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6427 ,  C12N1/16 ,  C12N9/1029 ,  C12P7/6463 ,  C12P7/6472 ,  C12Y203/01023 ,  Y02P20/52

    摘要： Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.

    摘要翻译： 本文公开了重组微生物细胞，其产生包含以干细胞重量的重量百分比测量的至少28％的二十碳五烯酸（EPA）的油。 这些细胞可以包含编码活性酰基-CoA：溶血卵磷脂酰胆碱酰基转移酶（LPCAT）的多核苷酸序列，其包含膜结合的O-酰基转移酶基序中的至少一个氨基酸突变。 此外，细胞可以包含编码Sou2山梨糖醇利用蛋白的内源多核苷酸序列和/或编码磷脂：二酰基甘油酰基转移酶（PDAT），δ-12去饱和酶，二氢-γ-亚麻酸的一种或多种多核苷酸的下调 （DGLA）合酶多酶，δ-8去饱和酶，丙二酰辅酶A合成酶（MCS）或酰基辅酶A：溶血磷脂酸酰基转移酶（LPAAT）。 还公开了使用重组微生物细胞产生含有ω-3多不饱和脂肪酸如EPA的油的方法。

    MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES
    3.
    发明申请
    MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES 有权
    突变型ACYL-CoA：LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES

    公开(公告)号：US20130337538A1

    公开(公告)日：2013-12-19

    申请号：US13916644

    申请日：2013-06-13

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Michael W. Bostick ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

    IPC分类号： C12N9/10

    CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/815 ,  Y02P20/52

    摘要： Mutant acyl-CoA:lysophosphatidylcholine acyltransferases [“LPCATs”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant LPCATs, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant LPCATs in oleaginous yeast, are also disclosed.

    摘要翻译： 具有将酰基-CoA + 1-酰基-sn-甘油基-3-磷酸胆碱转化为CoA + 1,2-二酰基-sn-甘油-3-磷酸胆碱（EC）的能力的突变型酰基-CoA：溶血磷脂酰胆碱酰基转移酶[“LPCAT”] 2.3.1.23）。 还公开了分离的核酸片段和包含编码突变体LPCAT的片段的重组构建体，以及在含油酵母中使用这些突变体LPCATs制备长链多不饱和脂肪酸的方法[“PUFA”]。

    PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
    5.
    发明申请
    PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES 有权
    通过ACYL-CoA的共表达生产聚氨酯脂肪酸：赖氨酸磷脂酰胆碱酯酶和磷脂酰胆碱：二乙酰胆碱酯酶

    公开(公告)号：US20130337514A1

    公开(公告)日：2013-12-19

    申请号：US13916628

    申请日：2013-06-13

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Michael W. Bostick ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  C12N15/81

    CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/815 ,  Y02P20/52

    摘要： Acyl-CoA:lysophosphatidylcholine acyltransferase [“LPCAT”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase [“PDAT”] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids [“PUFAs”].

    摘要翻译： 酰基-CoA：具有将酰基-CoA + 1-酰基-sn-甘油-3-磷酸胆碱转化成CoA + 1,2-二酰基-sn-甘油-3-磷酸胆碱（EC2.3）的能力的溶血磷脂酰胆碱酰基转移酶[“LPCAT” 本文公开的本文公开了与磷脂（例如磷脂酰胆碱）的sn-2位转移脂肪酰基的能力的磷脂：二酰基甘油酰基转移酶[“PDAT”]的过度表达的过表达; 到1,2-二酰基甘油[EC2.3.1.158]的sn-3位置，从而导致溶血磷脂和TAG。 这些酶在重组微生物宿主细胞中的共表达导致长链多不饱和脂肪酸的生产增加[“PUFA”]。