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    Production of polyunsaturated fatty acids by coexpression of acyl-CoA:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
    1.
    发明授权
    Production of polyunsaturated fatty acids by coexpression of acyl-CoA:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases 有权
    通过共表达酰基辅酶A生产多不饱和脂肪酸：溶血磷脂酰胆碱酰基转移酶和磷脂：二酰基甘油酰基转移酶

    公开(公告)号：US09416382B2

    公开(公告)日：2016-08-16

    申请号：US13916628

    申请日：2013-06-13

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Michael W Bostick ,  Narendra S Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

    IPC分类号： C12N1/21 ,  C12N1/15 ,  C12P7/64 ,  C12N15/81 ,  C12N9/10

    CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/815 ,  Y02P20/52

    摘要： Acyl-CoA:lysophosphatidylcholine acyltransferase [“LPCAT”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase [“PDAT”] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids [“PUFAs”].

    摘要翻译： 酰基-CoA：具有将酰基-CoA + 1-酰基-sn-甘油-3-磷酸胆碱转化成CoA + 1,2-二酰基-sn-甘油-3-磷酸胆碱（EC2.3）的能力的溶血磷脂酰胆碱酰基转移酶[“LPCAT” 本文公开的本文公开了与磷脂（例如磷脂酰胆碱）的sn-2位转移脂肪酰基的能力的磷脂：二酰基甘油酰基转移酶[“PDAT”]的过度表达的过表达; 到1,2-二酰基甘油[EC2.3.1.158]的sn-3位置，从而导致溶血磷脂和TAG。 这些酶在重组微生物宿主细胞中的共表达导致长链多不饱和脂肪酸的生产增加[“PUFA”]。

    Peroxisome biogenesis factor protein (PEX) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms
    3.
    发明授权
    Peroxisome biogenesis factor protein (PEX) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms 有权
    过氧化物酶体生物因子蛋白（PEX）改变多不饱和脂肪酸和油脂真核生物中总脂质含量的破坏

    公开(公告)号：US08822185B2

    公开(公告)日：2014-09-02

    申请号：US13780532

    申请日：2013-02-28

    申请人： E I Du Pont De Nemours and Company

    发明人： Seung-Pyo Hong ,  Pamela L Sharpe ,  Zhixiong Xue ,  Narendra S Yadav ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  C12P1/00 ,  C12N9/10 ,  C12N9/02 ,  C12N15/81

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/815 ,  C12P7/6472

    摘要： Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins, are disclosed. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted.

    摘要翻译： 公开了通过改变过氧化物酶体生物发生因子（Pex）蛋白的活性而实现的增加多脂不饱和脂肪酸（PUFA）在总脂质部分和产生PUFA的油酸真核生物的油馏分中的量的方法。 在产生PUFA的油性真菌菌株中染色体Pex3基因，Pex10p基因或Pex16p基因的破坏导致在总脂质分数中作为总脂肪酸的百分比和干细胞重量的百分比增加的PUFA的量 并且与其天然Pex蛋白未被破坏的亲本菌株相比，在菌株的油馏分中。

    MUTANT DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
    5.
    发明申请
    MUTANT DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS 有权
    MUTANT DELTA-5 DESATURASES及其在制备聚氨酯脂肪酸中的应用

    公开(公告)号：US20130122558A1

    公开(公告)日：2013-05-16

    申请号：US13736475

    申请日：2013-01-08

    申请人： E I Du Pont De Nemours And Company

    发明人： Quinn Qun Zhu ,  Dana M. Walters Pollak

    IPC分类号： C12N9/00 ,  C12P7/64

    CPC分类号： C12N9/0071 ,  C12P7/6427

    摘要： The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant Δ5 desaturases in oleaginous yeast, are disclosed.

    摘要翻译： 本发明涉及具有将dihomo-γ-亚麻酸[DGLA; 20：3ω-6]花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]，并且其在细胞色素b5样结构域的HPGG基序内具有至少一个突变。 公开了分离的核酸片段和包含编码Δ5去饱和酶的片段的重组构建体，以及在含油酵母中使用这些突变型Δ5去饱和酶制备长链多不饱和脂肪酸的方法。

    DOWN-REGULATION OF A POLYNUCLEOTIDE ENCODING A SOU2 SORBITOL UTILIZATION PROTEIN TO MODIFY LIPID PRODUCTION IN MICROBIAL CELLS
    8.
    发明申请
    DOWN-REGULATION OF A POLYNUCLEOTIDE ENCODING A SOU2 SORBITOL UTILIZATION PROTEIN TO MODIFY LIPID PRODUCTION IN MICROBIAL CELLS 有权
    编码SOU2 SORBITOL利用蛋白的多核苷酸的下调调节微生物细胞中的脂肪生产

    公开(公告)号：US20140220645A1

    公开(公告)日：2014-08-07

    申请号：US14132051

    申请日：2013-12-18

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Quinn Qun Zhu ,  Seung-Pyo Hong ,  Dongming Xie ,  Zhixiong Xue ,  Hyeryoung Yoon ,  Michael Dauner

    IPC分类号： C12P7/64

    CPC分类号： C12N9/0006 ,  C12N9/0036 ,  C12N15/815 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y101/01289 ,  Y02P20/52

    摘要： Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.

    摘要翻译： 本文公开了重组微生物细胞，其包含（i）编码Sou2山梨糖醇利用蛋白的内源多核苷酸序列的下调，和（ii）多不饱和脂肪酸（PUFA）生物合成途径。 编码Sou2山梨糖醇利用蛋白的多核苷酸序列的下调可以增加微生物细胞的脂质含量和/或降低由微生物细胞产生的糖醇的总量。 还公开了使用重组微生物细胞产生含有ω-3多不饱和脂肪酸如EPA的油的方法。