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    Production of polyunsaturated fatty acids by coexpression of acyl-CoA:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
    3.
    发明授权
    Production of polyunsaturated fatty acids by coexpression of acyl-CoA:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases 有权
    通过共表达酰基辅酶A生产多不饱和脂肪酸：溶血磷脂酰胆碱酰基转移酶和磷脂：二酰基甘油酰基转移酶

    公开(公告)号：US09416382B2

    公开(公告)日：2016-08-16

    申请号：US13916628

    申请日：2013-06-13

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Michael W Bostick ,  Narendra S Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

    IPC分类号： C12N1/21 ,  C12N1/15 ,  C12P7/64 ,  C12N15/81 ,  C12N9/10

    CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/815 ,  Y02P20/52

    摘要： Acyl-CoA:lysophosphatidylcholine acyltransferase [“LPCAT”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase [“PDAT”] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids [“PUFAs”].

    摘要翻译： 酰基-CoA：具有将酰基-CoA + 1-酰基-sn-甘油-3-磷酸胆碱转化成CoA + 1,2-二酰基-sn-甘油-3-磷酸胆碱（EC2.3）的能力的溶血磷脂酰胆碱酰基转移酶[“LPCAT” 本文公开的本文公开了与磷脂（例如磷脂酰胆碱）的sn-2位转移脂肪酰基的能力的磷脂：二酰基甘油酰基转移酶[“PDAT”]的过度表达的过表达; 到1,2-二酰基甘油[EC2.3.1.158]的sn-3位置，从而导致溶血磷脂和TAG。 这些酶在重组微生物宿主细胞中的共表达导致长链多不饱和脂肪酸的生产增加[“PUFA”]。

    Nucleic acids encoding mutant acyl-CoA:lysophosphatidylcholine acyltransferases
    6.
    发明授权
    Nucleic acids encoding mutant acyl-CoA:lysophosphatidylcholine acyltransferases 有权
    编码突变型酰基辅酶A的核酸：溶血磷脂酰胆碱酰基转移酶

    公开(公告)号：US09347075B2

    公开(公告)日：2016-05-24

    申请号：US13916644

    申请日：2013-06-13

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Michael W Bostick ,  Narendra S Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

    IPC分类号： C07H21/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/81 ,  C12P7/64 ,  C12N9/10

    CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/815 ,  Y02P20/52

    摘要： Mutant acyl-CoA:lysophosphatidylcholine acyltransferases [“LPCATs”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant LPCATs, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant LPCATs in oleaginous yeast, are also disclosed.

    摘要翻译： 具有将酰基-CoA + 1-酰基-sn-甘油基-3-磷酸胆碱转化为CoA + 1,2-二酰基-sn-甘油-3-磷酸胆碱（EC）的能力的突变型酰基-CoA：溶血磷脂酰胆碱酰基转移酶[“LPCAT”] 2.3.1.23）。 还公开了分离的核酸片段和包含编码突变体LPCAT的片段的重组构建体，以及在含油酵母中使用这些突变体LPCATs制备长链多不饱和脂肪酸的方法[“PUFA”]。

    PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
    7.
    发明申请
    PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES 有权
    通过ACYL-CoA的共表达生产聚氨酯脂肪酸：赖氨酸磷脂酰胆碱酯酶和磷脂酰胆碱：二乙酰胆碱酯酶

    公开(公告)号：US20130337514A1

    公开(公告)日：2013-12-19

    申请号：US13916628

    申请日：2013-06-13

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Michael W. Bostick ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  C12N15/81

    CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/815 ,  Y02P20/52

    摘要： Acyl-CoA:lysophosphatidylcholine acyltransferase [“LPCAT”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase [“PDAT”] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids [“PUFAs”].

    摘要翻译： 酰基-CoA：具有将酰基-CoA + 1-酰基-sn-甘油-3-磷酸胆碱转化成CoA + 1,2-二酰基-sn-甘油-3-磷酸胆碱（EC2.3）的能力的溶血磷脂酰胆碱酰基转移酶[“LPCAT” 本文公开的本文公开了与磷脂（例如磷脂酰胆碱）的sn-2位转移脂肪酰基的能力的磷脂：二酰基甘油酰基转移酶[“PDAT”]的过度表达的过表达; 到1,2-二酰基甘油[EC2.3.1.158]的sn-3位置，从而导致溶血磷脂和TAG。 这些酶在重组微生物宿主细胞中的共表达导致长链多不饱和脂肪酸的生产增加[“PUFA”]。

    MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES
    8.
    发明申请
    MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES 有权
    突变型ACYL-CoA：LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES

    公开(公告)号：US20130337538A1

    公开(公告)日：2013-12-19

    申请号：US13916644

    申请日：2013-06-13

    申请人： E I DU PONT DE NEMOURS AND COMPANY

    发明人： Michael W. Bostick ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

    IPC分类号： C12N9/10

    CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/815 ,  Y02P20/52

    摘要： Mutant acyl-CoA:lysophosphatidylcholine acyltransferases [“LPCATs”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant LPCATs, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant LPCATs in oleaginous yeast, are also disclosed.

    摘要翻译： 具有将酰基-CoA + 1-酰基-sn-甘油基-3-磷酸胆碱转化为CoA + 1,2-二酰基-sn-甘油-3-磷酸胆碱（EC）的能力的突变型酰基-CoA：溶血磷脂酰胆碱酰基转移酶[“LPCAT”] 2.3.1.23）。 还公开了分离的核酸片段和包含编码突变体LPCAT的片段的重组构建体，以及在含油酵母中使用这些突变体LPCATs制备长链多不饱和脂肪酸的方法[“PUFA”]。