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公开(公告)号：US09475847B2
公开(公告)日：2016-10-25
申请号：US13800233
申请日：2013-03-13
发明人： Daniel J. Altier , Jennifer K. Barry , Carol A. Hendrick , Lu Liu , Phillip A. Patten , Claudia D. Perez-Ortega , Eric J. Schepers , Weiping Xie , Nasser Yalpani , Jianzhou Zhao , Xiaohong Zhong , Genhai Zhu
IPC分类号： C07K14/225 , A01N37/46 , C07K14/195 , A01N63/02 , C12N15/82
CPC分类号： A01N37/46 , A01N63/02 , C07K14/195 , C07K14/225 , C07K2319/00 , C07K2319/21 , C12N15/8205 , C12N15/8207 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译： 提供了控制害虫的组成和方法。 该方法涉及用编码杀虫蛋白的核酸序列转化生物体。 特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。 因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。 组合物是杀虫的核酸和细菌物种的蛋白质。 这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，包括植物，作为分离其他同源（或部分同源的）基因的探针。 杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群并生产具有杀虫活性的组合物。
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公开(公告)号：US10563221B2
公开(公告)日：2020-02-18
申请号：US16001133
申请日：2018-06-06
发明人： Lu Liu , Jessica O'Rear , Young-Jun Park , Barbara Rosen , Ute Schellenberger , Eric Schepers , Jun-Zhi Wei , Weiping Xie , Nasser Yalpani , Xiaohong Zhong , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US20140033361A1
公开(公告)日：2014-01-30
申请号：US13800233
申请日：2013-03-13
发明人： Daniel J. Altier , Jennifer K. Barry , Carol A. Hendrick , Lu Liu , Phillip A. Patten , Claudia D. Perez-Ortega , Eric J. Schepers , Weiping Xie , Nasser Yalpani , Jianzhou Zhao , Xiaohong Zhong , Genhai Zhu
IPC分类号： C07K14/195 , C12N15/82 , A01N63/02
CPC分类号： A01N37/46 , A01N63/02 , C07K14/195 , C07K14/225 , C07K2319/00 , C07K2319/21 , C12N15/8205 , C12N15/8207 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译： 提供了控制害虫的组成和方法。 该方法涉及用编码杀虫蛋白的核酸序列转化生物体。 特别地，所述核酸序列可用于制备具有杀虫活性的植物和微生物。 因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。 组合物是杀虫的核酸和细菌物种的蛋白质。 这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，包括植物，作为分离其他同源（或部分同源的）基因的探针。 杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群并生产具有杀虫活性的组合物。
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公开(公告)号：US11840701B2
公开(公告)日：2023-12-12
申请号：US17660990
申请日：2022-04-27
发明人： Jennifer Kara Barry , James J English , Kevin A Fengler , Zhenglin Hou , Lu Liu , Eric Schepers , Jeffrey Sopa , Ingrid Udranszky
IPC分类号： C12N15/82 , C07K14/415 , A01N65/04
CPC分类号： C12N15/8286 , A01N65/04 , C07K14/415 , Y02A40/146
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US11208440B2
公开(公告)日：2021-12-28
申请号：US15557384
申请日：2016-03-03
发明人： Lu Liu , Jarred Kenneth Oral , Brad Poland , Ute Schellenberger
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity. Structure based methods for engineering insecticidal proteins of the disclosure to have modified physical properties are provided.
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公开(公告)号：US10667524B2
公开(公告)日：2020-06-02
申请号：US15022109
申请日：2014-09-11
发明人： Scott Diehn , James English , Lu Liu , Azalea Ong , Jarred Oral , Barbara Rosen , Ute Schellenberger , Ingrid Udranszky , Jun-Zhi Wei , Weiping Xie , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US10150956B2
公开(公告)日：2018-12-11
申请号：US15867385
申请日：2018-01-10
发明人： Ericka Bermudez , Andrew Mark Cigan , James J English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-Bin Liu , Azalea S Ong , Sergei Svitashev , Joshua K Young
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
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8.发明申请公开(公告)号：US20150184173A1
公开(公告)日：2015-07-02
申请号：US14398592
申请日：2013-05-01
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-bin Liu , Azalea Ong , Sergei Svitashev , Joshua K. Young
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
摘要翻译： 提供了包含具有大范围核酸酶活性的多核苷酸和多肽的组合物和方法。 还提供了具有大范围核酸酶序列的核酸构建体，酵母，植物，植物细胞，外植体，种子和颗粒。 提供了使用大范围核酸酶序列的各种方法。 这样的方法包括例如用于生产在宽温度范围内具有增加的活性的大范围核酸酶的方法，用于生产酵母，植物，植物细胞，外植体或包含具有增加的活性的大范围核酸酶的种子的方法。
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公开(公告)号：US20130125256A1
公开(公告)日：2013-05-16
申请号：US13748623
申请日：2013-01-24
发明人： Stephen M. Allen , Kanwarpal Singh Dhugga , Kevin Fengler , Howard P. Hershey , Lu Liu , Victor Llaca , Dale F. Loussaert , Xiaomu Niu , Haiyin Wang
IPC分类号： C12N15/82
CPC分类号： C12N15/8243 , C07K14/415 , C12N15/8227 , C12N15/8238 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated nucleic acid fragments encoding high affinity nitrate transport components. The invention also relates to the construction of recombinant DNA constructs encoding all or a portion of nitrate transport components, in sense or antisense orientation, wherein expression of the recombinant DNA construct may alter levels of the nitrate transport components in a transformed host cell.
摘要翻译： 本发明涉及编码高亲和力硝酸盐转运组分的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分硝酸盐转运组分的重组DNA构建体，其中重组DNA构建体的表达可改变转化的宿主细胞中硝酸盐转运组分的水平。
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公开(公告)号：US12116588B2
公开(公告)日：2024-10-15
申请号：US17973124
申请日：2022-10-25
发明人： Lu Liu , Amy Lum , Azalea S. Ong , Eric Schepers , Ingrid Udranszky , Xiaohong Zhong
IPC分类号： C12N15/82 , A01N63/50 , C07K14/415
CPC分类号： C12N15/8286 , A01N63/50 , C07K14/415 , C07K2319/00
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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