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公开(公告)号：US5276268A
公开(公告)日：1994-01-04
申请号：US736316
申请日：1991-07-29
申请人： Eckhard Strauch , Walter Arnold , Renate Alijah , Wolfgang Wohlleben , Alfred Puhler , Peter Eckes , Gunter Donn , Eugen Uhlmann , Friedrich Hein , Friedrich Wengenmayer
发明人： Eckhard Strauch , Walter Arnold , Renate Alijah , Wolfgang Wohlleben , Alfred Puhler , Peter Eckes , Gunter Donn , Eugen Uhlmann , Friedrich Hein , Friedrich Wengenmayer
CPC分类号： C12N9/1029 , C12N15/64 , C12N15/8209 , C12N15/8277 , C12P41/007
摘要： The phosphinothricin (PTC)-resistance gene isolated from the genome of Streptomyces viridochromogenes DSM 40736 is, after adaptation to the codon usage in plants, synthesized and incorporated into gene structures which make plants resistant to PTC after expression therein.
摘要翻译： 从植物链霉菌（Streptomyces viridochromogenes）DSM 40736的基因组中分离出的膦丝菌素（PTC） - 抗性基因，在适应于植物中的密码子使用之后，合成并掺入基因结构中，使得植物在其表达后能耐PTC。
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公开(公告)号：US5879903A
公开(公告)日：1999-03-09
申请号：US479717
申请日：1995-06-07
申请人： Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
发明人： Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
CPC分类号： C12P41/007 , C12N15/64 , C12N15/8209 , C12N15/8277 , C12N9/1029
摘要： Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.
摘要翻译： 选择链霉菌（Streptomyces viridochromogenes）DSM 4112用于抗磷酰基丙氨酰 - 丙氨酸（PTT）的抗性导致PTT抗性选择。 通过用BamHI切割，克隆尺寸为4.0kb的片段和选择PTT抗性，从这些选择物的总DNA获得载有膦丝菌蛋白（PTC）抗性基因的DNA片段。 该基因适用于生产耐PTC抗性植物，以及作为抗性标记物和L-型外消旋PTC的选择性N-乙酰化。
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公开(公告)号：US5637489A
公开(公告)日：1997-06-10
申请号：US487695
申请日：1995-06-07
申请人： Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
发明人： Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
CPC分类号： C12N9/1029 , C12N15/64 , C12N15/8209 , C12N15/8277 , C12P41/007
摘要： Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.
摘要翻译： 选择链霉菌（Streptomyces viridochromogenes）DSM 4112用于抗磷酰基丙氨酰 - 丙氨酸（PTT）的抗性导致PTT抗性选择。 通过用BamHI切割，克隆尺寸为4.0kb的片段和选择PTT抗性，从这些选择物的总DNA获得载有膦丝菌蛋白（PTC）抗性基因的DNA片段。 该基因适用于生产耐PTC抗性植物，以及作为抗性标记物和L-型外消旋PTC的选择性N-乙酰化。
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公开(公告)号：US5273894A
公开(公告)日：1993-12-28
申请号：US795275
申请日：1991-11-20
申请人： Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
发明人： Eckhard Strauch , Wolfgang Wohlleben , Walter Arnold , Renate Alijah , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt , Susanne Grabley , Dieter Brauer , Klaus Bartsch
CPC分类号： C12P41/007 , C12N15/64 , C12N15/8209 , C12N15/8277 , C12N9/1029
摘要： Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.
摘要翻译： 选择链霉菌（Streptomyces viridochromogenes）DSM 4112用于抗磷酰基丙氨酰 - 丙氨酸（PTT）的抗性导致PTT抗性选择。 通过用BamHI切割，克隆尺寸为4.0kb的片段和选择PTT抗性，从这些选择物的总DNA获得载有膦丝菌蛋白（PTC）抗性基因的DNA片段。 该基因适用于生产耐PTC抗性植物，以及作为抗性标记物和L-型外消旋PTC的选择性N-乙酰化。
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5.发明授权Deacetylase genes for the production of phosphinothricin or phosphin-othricyl-alanyl-alanine, process for their isolation, and their use 失效用于生产膦丝菌素或次膦酰基 - 丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途
公开(公告)号：US5767371A
公开(公告)日：1998-06-16
申请号：US459255
申请日：1995-06-02
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H5/00 , C12N5/14 , C12N15/31 , C12N15/32
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use. The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途。 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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6.发明授权Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use 失效用于生产膦丝菌素或膦酰丙酰丙氨酸 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途
公开(公告)号：US5767370A
公开(公告)日：1998-06-16
申请号：US459254
申请日：1995-06-02
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H5/00 , C12N5/14 , C12N15/31 , C12N15/52
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use. The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途。 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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7.发明授权Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, process for their isolation, and their use 失效用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途
公开(公告)号：US5668297A
公开(公告)日：1997-09-16
申请号：US461179
申请日：1995-06-05
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H4/00
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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8.发明授权Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine processes for their isolation and their use 失效用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸方法的脱乙酰酶基因用于分离和使用
公开(公告)号：US5650310A
公开(公告)日：1997-07-22
申请号：US458912
申请日：1995-06-02
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H4/00
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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公开(公告)号：US4965207A
公开(公告)日：1990-10-23
申请号：US83805
申请日：1987-08-11
IPC分类号： C12N15/09 , C07K14/36 , C12N1/21 , C12N15/00 , C12N15/65 , C12N15/76 , C12P21/00 , C12R1/465
CPC分类号： C12N15/76 , C07K14/36 , C12N15/65 , Y10S435/886
摘要： Intensely crimson colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of Streptomyces lividans. Re-isolation of the plasmid DNA and cutting with BamHI results in fragments about 3.4 to 9 kb in length, on which is located the gene which determines production of the coloring agent. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.
摘要翻译： 通过总DNA的全部消化，克隆到合适的载体和变形链霉菌（Streptomyces lividans）中，从Streptomyces coelicolor DSM 3030获得强烈的深红色菌落。 重新分离质粒DNA并用BamHI切割产生长度约3.4至9kb的片段，其位于确定着色剂生产的基因。 该基因在链霉菌中作为标记物，特别是作为灭活标记物是合适的。
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公开(公告)号：US4963496A
公开(公告)日：1990-10-16
申请号：US83806
申请日：1987-08-11
摘要： Intensely blue-black colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of a streptomycetes recipient strain. Re-isolation of the plasmid DNA and cutting with BamHI results in a 5.5 kb fragment on which the gene for the coloring agent is located. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.
摘要翻译： 通过总DNA的总消化，克隆到合适的载体和链霉菌受体菌株的转化中，从Streptomyces coelicolor DSM 3030获得强烈的蓝黑色菌落。 重新分离质粒DNA并用BamHI切割产生5.5kb片段，其上着色剂的基因位于其上。 该基因在链霉菌中作为标记物，特别是作为灭活标记物是合适的。
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