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1.发明授权Deacetylase genes for the production of phosphinothricin or phosphin-othricyl-alanyl-alanine, process for their isolation, and their use 失效用于生产膦丝菌素或次膦酰基 - 丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途
公开(公告)号：US5767371A
公开(公告)日：1998-06-16
申请号：US459255
申请日：1995-06-02
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H5/00 , C12N5/14 , C12N15/31 , C12N15/32
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use. The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途。 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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2.发明授权Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use 失效用于生产膦丝菌素或膦酰丙酰丙氨酸 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途
公开(公告)号：US5767370A
公开(公告)日：1998-06-16
申请号：US459254
申请日：1995-06-02
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H5/00 , C12N5/14 , C12N15/31 , C12N15/52
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use. The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途。 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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3.发明授权Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, process for their isolation, and their use 失效用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸的脱乙酰酶基因，其分离过程及其用途
公开(公告)号：US5668297A
公开(公告)日：1997-09-16
申请号：US461179
申请日：1995-06-05
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H4/00
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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4.发明授权Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine processes for their isolation and their use 失效用于生产膦丝菌素或膦酰基丙氨酰 - 丙氨酸方法的脱乙酰酶基因用于分离和使用
公开(公告)号：US5650310A
公开(公告)日：1997-07-22
申请号：US458912
申请日：1995-06-02
申请人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
发明人： Inge Broer , Doris Hillemann , Alfred Puhler , Wolfgang Wohlleben , Gunter Donn , Hubert Mullner , Klaus Bartsch
IPC分类号： A01H1/00 , C12N9/14 , C12N15/09 , C12N15/55 , C12N15/60 , C12N15/82 , C12R1/19 , C12R1/465 , A01H4/00
CPC分类号： C12N15/8209 , C12N15/8261 , C12N9/14
摘要： The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.
摘要翻译： 本发明涉及脱乙酰酶基因，其分离方法及其用途，特别是使用组织特异性启动子生产转基因植物。 有可能以有针对性的方式防止这些植物中某些部位的发育。 借助于脱乙酰酶基因，还可以在转基因植物中鉴定和分离组织特异性启动子。
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公开(公告)号：US5276268A
公开(公告)日：1994-01-04
申请号：US736316
申请日：1991-07-29
申请人： Eckhard Strauch , Walter Arnold , Renate Alijah , Wolfgang Wohlleben , Alfred Puhler , Peter Eckes , Gunter Donn , Eugen Uhlmann , Friedrich Hein , Friedrich Wengenmayer
发明人： Eckhard Strauch , Walter Arnold , Renate Alijah , Wolfgang Wohlleben , Alfred Puhler , Peter Eckes , Gunter Donn , Eugen Uhlmann , Friedrich Hein , Friedrich Wengenmayer
CPC分类号： C12N9/1029 , C12N15/64 , C12N15/8209 , C12N15/8277 , C12P41/007
摘要： The phosphinothricin (PTC)-resistance gene isolated from the genome of Streptomyces viridochromogenes DSM 40736 is, after adaptation to the codon usage in plants, synthesized and incorporated into gene structures which make plants resistant to PTC after expression therein.
摘要翻译： 从植物链霉菌（Streptomyces viridochromogenes）DSM 40736的基因组中分离出的膦丝菌素（PTC） - 抗性基因，在适应于植物中的密码子使用之后，合成并掺入基因结构中，使得植物在其表达后能耐PTC。
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6.发明授权
公开(公告)号：US5792926A
公开(公告)日：1998-08-11
申请号：US458695
申请日：1995-06-02
申请人： Rudolf Schneider , Gunter Donn , Hubert Mullner
发明人： Rudolf Schneider , Gunter Donn , Hubert Mullner
CPC分类号： C07K14/005 , C12N15/8277 , C12N15/8283 , C12N9/1029 , C12N2770/00022
摘要： Virus genes, such as coat protein genes, which confer viral resistance or bring about a reduction in the signs of infection by the corresponding virus can be combined with herbicide-resistance genes, such as the phosphinothricin resistance gene, for the transformation of plants. A combination of a viral gene and a herbicide-resistance gene facilitates the selection of transgenic plants. In addition, in practical field cultivation, the vitality of the plants is increased by the virus tolerance, and an improved plant protection is possible due to the herbicide resistance gene.
摘要翻译： 赋予病毒抗性或引起相应病毒感染迹象降低的病毒基因（例如外壳蛋白基因）可以与用于转化植物的除草剂抗性基因（例如膦丝菌素抗性基因）结合。 病毒基因和除草剂抗性基因的组合促进了转基因植物的选择。 此外，在实际的田间种植中，植物的活力由于病毒耐受性而增加，并且由于除草剂抗性基因可以改善植物保护。
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公开(公告)号：US5077399A
公开(公告)日：1991-12-31
申请号：US515671
申请日：1990-04-27
申请人： Dieter Brauer , Klaus Bartsch , Gunter Donn
发明人： Dieter Brauer , Klaus Bartsch , Gunter Donn
IPC分类号： A01H5/00 , A01N63/00 , C02F3/34 , C12N9/10 , C12N15/00 , C12N15/09 , C12N15/82 , C12R1/01 , C12R1/05 , C12R1/38 , C12R1/425
CPC分类号： C12N9/1029 , C12N15/8277 , Y10S435/829
摘要： Phosphinothricin (PTC) selection of bacteria which are not fungus-like yields PTC-resistant selectants. The DNA fragment which carries the resistance gene is obtained from the complete DNA of these selectants by constructing a gene bank and screening for chemical modification of PTC. The resistance gene can be localized to a fragment which is 2 kb in size, and selection for PTC resistance. This gene is suitable for producing PTC-resistant plants and propagation material thereof, and as a resistance marker as well. Microorganisms which contain this PTC-resistance gene can be used in sewage treatment plants.
摘要翻译： 非真菌类细菌的次氯氰菊酯（PTC）选择产生耐PTC抗性选择剂。 携带抗性基因的DNA片段通过构建基因库并筛选PTC的化学修饰从这些选择物的完整DNA获得。 抗性基因可以定位于2kb大小的片段，并选择PTC抗性。 该基因适用于生产耐PTC的植物及其繁殖材料，也可用作抗性标记。 含有该PTC抗性基因的微生物可用于污水处理厂。
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公开(公告)号：US5767367A
公开(公告)日：1998-06-16
申请号：US371764
申请日：1995-01-12
申请人： Denes Dudits , Sandor Morocz , Janos Nemeth , Gunter Donn
发明人： Denes Dudits , Sandor Morocz , Janos Nemeth , Gunter Donn
CPC分类号： A01H5/10 , A01H4/00 , A01H4/005 , C12N15/8206 , C12N15/8277 , C12N5/04 , C12N9/1029
摘要： Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.
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公开(公告)号：US5723762A
公开(公告)日：1998-03-03
申请号：US465526
申请日：1995-06-05
申请人： Denes Dudits , Katalin Paulovics , Katalin Kalman , Janos Gyorgyey , Ferenc Nagy , Laszlo Bako , Gabor Horvath , Peter Eckes , Gunter Donn
发明人： Denes Dudits , Katalin Paulovics , Katalin Kalman , Janos Gyorgyey , Ferenc Nagy , Laszlo Bako , Gabor Horvath , Peter Eckes , Gunter Donn
CPC分类号： C12N9/93 , C12N15/8261 , C12N15/8277
摘要： The gene asnA which encodes a prokaryotic ammonium-specific asparagine synthetase (ASN-A) can be introduced into plant cells. Such transformed cells and plants developed therefrom not only tolerate glutamine synthetase inhibitors but are effectively stimulated by such herbicides.
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公开(公告)号：US5145777A
公开(公告)日：1992-09-08
申请号：US700116
申请日：1991-05-09
申请人： Howard M. Goodman , Gunter Donn
发明人： Howard M. Goodman , Gunter Donn
摘要： A plant cell which is resistant to a herbicidal glutamine synthetase inhibitor, wherein the resistance is caused by levels of GS activity which, when present in an otherwise herbicidal GS inhibitor sensitive plant cell, render the cell substantially resistant to the herbicidal GS-inhibitor.
摘要翻译： 对除草谷氨酰胺合成酶抑制剂具有抗性的植物细胞，其中所述抗性是由GS活性的水平引起的，GS活性当存在于另外除草的GS抑制剂敏感性植物细胞中时，使细胞对除草GS-抑制剂基本上具有抗性。
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