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公开(公告)号：US6127175A
公开(公告)日：2000-10-03
申请号：US875223
申请日：1997-07-17
申请人： Emmanuelle Vigne , Michel Perricaudet , Jean-Fran.cedilla.ois Dedieu , Cecile Orsini , Patrice Yeh , Martine Latta , Edouard Prost
发明人： Emmanuelle Vigne , Michel Perricaudet , Jean-Fran.cedilla.ois Dedieu , Cecile Orsini , Patrice Yeh , Martine Latta , Edouard Prost
IPC分类号： C12N15/09 , C07K14/075 , C12N5/10 , C12N7/00 , C12N7/04 , C12N15/00 , C12N15/34 , C12N15/861 , C12N15/864 , C12R1/92 , C12P21/06 , C12N7/01
CPC分类号： C12N15/86 , C12N7/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10362 , C12N2750/14143 , C12N2830/002
摘要： The invention relates to cells usable for the production of defective adenoviruses comprising, inserted into their genome, a portion of the region E4 of an adenovirus genome carrying the reading phase ORF6 under the control of a functional promoter.
摘要翻译： PCT No.PCT / FR96 / 00088 Sec。 371日期1997年7月17日 102（e）日期1997年7月17日PCT 1996年1月19日PCT PCT。 公开号WO96 / 22378 日期1996年7月25日本发明涉及可用于产生缺陷型腺病毒的细胞，其包含在功能性启动子控制下携带阅读期ORF6的腺病毒基因组区域E4的一部分插入其基因组中。
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公开(公告)号：US07033826B2
公开(公告)日：2006-04-25
申请号：US10121616
申请日：2002-04-15
申请人： Michel Perricaudet , Martine Latta , Edouard Prost , Patrice Yeh , Cécile Orsini , Emmanuelle Vigne
发明人： Michel Perricaudet , Martine Latta , Edouard Prost , Patrice Yeh , Cécile Orsini , Emmanuelle Vigne
IPC分类号： C12N15/861 , C12N15/864 , C12N15/63
CPC分类号： C12N15/86 , C12N15/63 , C12N2710/10343 , C12N2710/10344 , C12N2710/16622 , C12N2750/14122 , C12N2750/14143 , C12N2830/003 , C12N2830/15
摘要： A recombinant adenovirus in which the expression of a nucleic acid sequence coding for at least one homologous or heterologous gene of viral origin is placed under the control of an inducible promoter, is disclosed. The use of such recombinant adenoviruses for preparing AAVs, and a complementary cell line and preparation method therefor, are also disclosed. Furthermore, pharmaceutical compositions containing such an adenovirus are disclosed.
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3.发明授权Recombinant adenoviruses containing an inducible promoter controlling a gene of viral origin 失效含有控制病毒来源基因的诱导型启动子的重组腺病毒公开(公告)号：US06420170B1
公开(公告)日：2002-07-16
申请号：US08981354
申请日：1997-12-19
申请人： Michel Perricaudet , Martine Latta , Edouard Prost , Patrice Yeh , Cécile Orsini , Emmanuelle Vigne
发明人： Michel Perricaudet , Martine Latta , Edouard Prost , Patrice Yeh , Cécile Orsini , Emmanuelle Vigne
IPC分类号： C12N15861
CPC分类号： C12N15/86 , C12N15/63 , C12N2710/10343 , C12N2710/10344 , C12N2710/16622 , C12N2750/14122 , C12N2750/14143 , C12N2830/003 , C12N2830/15
摘要： A recombinant adenovirus in which the expression of a nucleic acid sequence coding for at least one homologous or heterologous gene of viral origin is placed under the control of an inducible promoter, is disclosed. The use of such recombinant adenoviruses for preparing AAVs, and a complementary cell line and preparation method therefor, are also disclosed. Furthermore, pharmaceutical compositions containing such an adenovirus are disclosed.
摘要翻译： 公开了将编码病毒来源的至少一个同源或异源基因的核酸序列的表达置于诱导型启动子控制下的重组腺病毒。 还公开了这样的重组腺病毒用于制备AAV的用途，以及互补细胞系及其制备方法。 此外，公开了含有这种腺病毒的药物组合物。
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公开(公告)号：US20060002893A1
公开(公告)日：2006-01-05
申请号：US11038980
申请日：2005-01-20
IPC分类号： A61K48/00 , C12N7/00 , C12N15/861
CPC分类号： C07K14/005 , A61K48/00 , C07K14/501 , C07K14/78 , C07K2319/00 , C12N9/6462 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2770/32622 , C12N2770/32634 , C12N2810/40 , C12N2810/55 , C12N2810/60 , C12N2810/6018 , C12N2810/80 , C12N2810/851 , C12N2810/855 , C12N2810/857 , C12Y304/21073
摘要： Modification of internal sites of the adenovirus fiber protein and hexon protein permit effective targeting of adenovirus vectors. Accessible sites to redirect adenovirus targeting were identified. The HVR5 loop of the hexon protein and the HI loop of the fiber protein (knob) were highly permissive for the insertion of foreign protein sequences, which apparently did not impact on the viability and productivity of corresponding viruses. Accessibility and functionality of the epitope strongly depend on the size of the neighboring spacers. Other results suggest that short targeting peptides can be effectively fused to the C-terminus of the fiber protein. In a specific embodiment, a series of adenovirus vectors modified at the HVR5 site, the fiber protein HI loop, or the fiber protein C-terminus to target urokinase-type plasminogen activator receptor bearing cells were prepared. Such vectors are particularly useful for targeting the vasculature, e.g., for gene therapy of cancers or cardiovascular conditions.
摘要翻译： 腺病毒纤维蛋白和六邻体蛋白的内部位点的修饰允许有效靶向腺病毒载体。 确定了用于重定向腺病毒靶向的可访问网站。 六邻体蛋白的HVR5环和纤维蛋白（旋钮）的HI环对于插入外源蛋白序列是高度允许的，其显然不影响相应病毒的活力和生产力。 表位的可访问性和功能性强烈地取决于相邻间隔物的尺寸。 其他结果表明，短目标肽可以有效融合到纤维蛋白的C末端。 在一个具体实施方案中，制备了在HVR5位点修饰的一系列腺病毒载体，纤维蛋白质HI环或纤维蛋白C末端以靶向尿激酶型纤溶酶原激活物受体的细胞。 这样的载体特别可用于靶向脉管系统，例如用于癌症或心血管病症的基因治疗。
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公开(公告)号：US06911199B2
公开(公告)日：2005-06-28
申请号：US09791524
申请日：2001-02-22
IPC分类号： C12N15/09 , A61K35/76 , A61K45/00 , A61K48/00 , A61P9/10 , A61P11/06 , A61P21/04 , A61P25/00 , A61P31/04 , A61P31/18 , A61P35/00 , A61P37/00 , C07K14/075 , C07K14/105 , C07K14/50 , C07K14/78 , C12N7/00 , C12N7/01 , C12N9/72 , C12N15/34 , C12N15/861 , C12R1/92 , C12N15/63
CPC分类号： C07K14/005 , A61K48/00 , C07K14/501 , C07K14/78 , C07K2319/00 , C12N9/6462 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2770/32622 , C12N2770/32634 , C12N2810/40 , C12N2810/55 , C12N2810/60 , C12N2810/6018 , C12N2810/80 , C12N2810/851 , C12N2810/855 , C12N2810/857 , C12Y304/21073
摘要： Modification of internal sites of the adenovirus fiber protein and hexon protein permit effective targeting of adenovirus vectors. Accessible sites to redirect adenovirus targeting were identified. The HVR5 loop of the hexon protein and the HI loop of the fiber protein (knob) were highly permissive for the insertion of foreign protein sequences, which apparently did not impact on the viability and productivity of corresponding viruses. Accessibility and functionality of the epitope strongly depend on the size of the neighboring spacers. Other results suggest that short targeting peptides can be effectively fused to the C-terminus of the fiber protein. In a specific embodiment, a series of adenovirus vectors modified at the HVR5 site, the fiber protein HI loop, or the fiber protein C-terminus to target urokinase-type plasminogen activator receptor bearing cells were prepared. Such vectors are particularly useful for targeting the vasculature, e.g., for gene therapy of cancers or cardiovascular conditions.
摘要翻译： 腺病毒纤维蛋白和六邻体蛋白的内部位点的修饰允许有效靶向腺病毒载体。 确定了用于重定向腺病毒靶向的可访问网站。 六邻体蛋白的HVR5环和纤维蛋白（旋钮）的HI环对于插入外源蛋白序列是高度允许的，其显然不影响相应病毒的活力和生产力。 表位的可访问性和功能性强烈地取决于相邻间隔物的尺寸。 其他结果表明，短目标肽可以有效融合到纤维蛋白的C末端。 在一个具体实施方案中，制备了在HVR5位点修饰的一系列腺病毒载体，纤维蛋白质HI环或纤维蛋白C末端以靶向尿激酶型纤溶酶原激活物受体的细胞。 这样的载体特别可用于靶向脉管系统，例如用于癌症或心血管病症的基因治疗。
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6.发明授权
公开(公告)号：US6033885A
公开(公告)日：2000-03-07
申请号：US702573
申请日：1996-09-12
IPC分类号： C12N15/09 , A61K9/08 , A61K35/76 , A61K38/00 , A61K38/22 , A61K38/43 , A61K38/46 , A61K48/00 , A61P9/10 , A61P25/00 , A61P25/02 , A61P25/28 , A61P31/12 , A61P35/00 , A61P37/04 , C07K14/775 , C12N5/10 , C12N7/00 , C12N7/01 , C12N7/04 , C12N15/861 , C12N15/864 , C12N15/00 , A01N63/00 , C12P1/00
CPC分类号： C12N15/86 , C07K14/775 , C12N7/00 , A61K38/00 , A61K48/00 , C12N2710/10343 , C12N2710/10344 , C12N2710/10362 , C12N2750/14143 , C12N2830/008
摘要： The present invention relates to recombinant adenoviruses having a cassette capable of integrating into the genome of infected cells, their preparation, pharmaceutical compositions containing them, and their use. In particular, the cassette contains at least one inverted terminal repeat (ITR) Sequence from AAV and a heterologous DNA Sequence.
摘要翻译： PCT No.PCT / FR95 / 00233 Sec。 371日期：1996年9月12日 102（e）日期1996年9月12日PCT提交1995年2月28日PCT公布。 第WO95 / 23867号公报 日期1995年9月8日本发明涉及具有能够整合入感染细胞的基因组的盒，其制备物，含有它们的药物组合物及其用途的重组腺病毒。 特别地，盒包含至少一个来自AAV的反向末端重复（ITR）序列和异源DNA序列。
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公开(公告)号：US5187261A
公开(公告)日：1993-02-16
申请号：US653195
申请日：1991-02-08
IPC分类号： C07K14/76 , C07K14/00 , C07K14/765 , C07K19/00 , C12N15/00 , C12N15/09 , C12N15/14 , C12N15/62 , C12N15/70 , C12P21/02 , C12R1/19
CPC分类号： C12N15/70 , C07K14/765 , C07K2319/00 , C07K2319/02
摘要： Mature human serum albumin is produced from a human serum albumin produced by a microbiological route in the form of fused protein ("pseudo-pro-HSA") containing an N-terminal peptide elongation.
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公开(公告)号：US5100784A
公开(公告)日：1992-03-31
申请号：US16651
申请日：1987-02-19
IPC分类号： C07K14/76 , C07K14/00 , C07K14/765 , C07K19/00 , C12N15/00 , C12N15/09 , C12N15/14 , C12N15/62 , C12N15/70 , C12P21/02 , C12R1/19
CPC分类号： C12N15/70 , C07K14/765 , C07K2319/00 , C07K2319/02
摘要： Mature human serum albumin is produced from a human serum albumin produced by a microbiological route in the form of fused protein ("pseudo-pro-HSA") containing an N-terminal peptide elongation.
摘要翻译： 通过微生物途径产生的人血清白蛋白是以含有N-末端肽伸长的融合蛋白（“假原HSA”）形式产生的。
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公开(公告)号：US4914027A
公开(公告)日：1990-04-03
申请号：US843725
申请日：1986-03-25
CPC分类号： C07K14/765 , C12N15/70 , C12N9/84
摘要： Human serum albumin is produced by culturing a bacterium (e.g. E. coli) capable of maintaining a plasmid containing an inducible promoter (e.g. P.sub.trp) upstream of the penicillin amidase promoter, the ribosome binding site of the penicillin amidase gene and the penicillin amidase signal peptide, fused with the structural gene for human serum albumin.
摘要翻译： 通过培养能够维持含有青霉素酰胺酶启动子上游的诱导型启动子（例如Ptrp）的质粒，青霉素酰胺酶基因的核糖体结合位点和青霉素酰胺酶信号肽的细菌（例如大肠杆菌）产生人血清白蛋白 与人血清白蛋白的结构基因融合。
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