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    Method for producing RNA viruses from cDNA
    3.
    发明授权
    Method for producing RNA viruses from cDNA 失效
    从cDNA生产RNA病毒的方法

    公开(公告)号：US06136570A

    公开(公告)日：2000-10-24

    申请号：US465250

    申请日：1995-06-05

    申请人： Vincent Racaniello ,  Joanne Marie Tatem ,  Carolyn L. Weeks-Levy

    发明人： Vincent Racaniello ,  Joanne Marie Tatem ,  Carolyn L. Weeks-Levy

    IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/13 ,  C07K14/105 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/09 ,  C12N15/43 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/19 ,  C12R1/91

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/32622

    摘要： The present invention relates to methods for producing RNA virus cDNA, methods for producing viable, RNA virus and viable, RNA virus produced by those methods. The invention also related to a novel RNA virus cDNA, recombinant DNA molecules containing that cDNA and hosts transformed with those recombinant cDNA molecules. This invention further related to novel methods for screening for variants of a strain 3 poliovirus. This invention also related to methods for increasing the attenuation of a strain poliovirus.This invention provides a vaccine useful for immunizing a subject, for example a human, against infectious poliovirus, wherein the vaccine comprises an effective amount of an RNA virus, produced by transforming a suitable host cell with a recombinant nucleic acid sequence which encodes for the virus: culturing the host cell under conditions which permit the production of virus: and isolating the virus so produced, effective to immunize the subject, and a suitable carrier. Further provided to this invention is a method of immunizing a subject such as a human against infectious poliovirus, wherein the method comprises administering the subject a suitable dose of the vaccine described hereinabove.

    摘要翻译： 本发明涉及用于生产RNA病毒cDNA的方法，通过这些方法产生的用于生产活的RNA病毒和可行的RNA病毒的方法。 本发明还涉及新型RNA病毒cDNA，含有该cDNA的重组DNA分子和用这些重组cDNA分子转化的宿主。 本发明还涉及用于筛选菌株3脊髓灰质炎病毒变体的新方法。 本发明还涉及用于增加菌株脊髓灰质炎病毒的衰减的方法。 本发明提供了可用于免疫针对感染性脊髓灰质炎病毒的受试者例如人的疫苗，其中疫苗包含有效量的RNA病毒，其通过用编码病毒的重组核酸序列转化合适的宿主细胞而产生 ：在允许产生病毒的条件下培养宿主细胞;分离如此产生的病毒，对受试者免疫有效和合适的载体。 进一步提供给本发明的方法是免疫针对感染性脊髓灰质炎病毒的人类对象，其中所述方法包括给予受试者合适剂量的上述疫苗。

    Encapsidated recombinant viral nucleic acid and methods of making and using same
    9.
    发明授权
    Encapsidated recombinant viral nucleic acid and methods of making and using same 失效
    封装的重组病毒核酸及其制备和使用方法

    公开(公告)号：US6063384A

    公开(公告)日：2000-05-16

    申请号：US987867

    申请日：1997-12-09

    申请人： Casey D. Morrow ,  Donna C. Porter ,  David C. Ansardi

    发明人： Casey D. Morrow ,  Donna C. Porter ,  David C. Ansardi

    IPC分类号： C07K14/105 ,  C07K14/16 ,  C07K14/705 ,  C12P21/02 ,  C12N15/43 ,  A61K39/13

    CPC分类号： C07K14/705 ,  C07K14/005 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/6075 ,  C07K2319/00 ,  C12N2740/16222 ,  C12N2770/32622 ,  C12N2770/32634

    摘要： The present invention pertains to a method of encapsidating a recombinant poliovirus nucleic acid to obtain a yield of encapsidated viruses which substantially comprises encapsidated recombinant poliovirus nucleic acid. The method of encapsidating a recombinant poliovirus nucleic acid includes contacting a host cell with a recombinant poliovirus nucleic acid which lacks the nucleotide sequence encoding at least a portion of a protein necessary for encapsidation and an expression vector comprising a nucleic acid which encodes at least a portion of one protein necessary for encapsidation under conditions appropriate for introduction of the recombinant poliovirus nucleic acid and the expression vector into the host cell and obtaining a yield of encapsidated viruses which substantially comprises an encapsidated recombinant poliovirus nucleic acid. A foreign nucleotide sequence is generally substituted for the nucleotide sequence of the poliovirus nucleic acid encoding at least a portion of a protein necessary for encapsidation. The invention further pertains to encapsidated recombinant poliovirus nucleic acids produced by the method of this invention and compositions containing the encapsidated or nonencapsidated recombinant poliovirus nucleic acid containing a foreign nucleotide sequence for use in a method of stimulating an immune response in a subject to the protein encoded by the foreign nucleotide sequence.

    摘要翻译： 本发明涉及一种包囊重组体脊髓灰质炎病毒核酸以获得包衣病毒的产量的方法，其基本上包含被包被的重组体脊髓灰质炎病毒核酸。 包裹重组体脊髓灰质炎病毒核酸的方法包括使宿主细胞与重组脊髓灰质炎病毒核酸接触，所述重组体脊髓灰质炎病毒核酸缺少编码至少一部分用于壳化所必需的蛋白质的核苷酸序列和包含编码至少一部分的核酸的表达载体 在适合于将重组脊髓灰质炎病毒核酸和表达载体导入宿主细胞的条件下进行壳化所必需的一种蛋白质，并获得基本上包含包被的重组脊髓灰质炎病毒核酸的包膜病毒的产量。 外源核苷酸序列通常代替编码壳体必需蛋白质的至少一部分的脊髓灰质炎病毒核酸的核苷酸序列。 本发明还涉及通过本发明的方法生产的包封的重组体脊髓灰质炎病毒核酸和含有含有外源核苷酸序列的包封的或非包封的重组体脊髓灰质炎病毒核酸的组合物，所述重组体脊髓灰质炎病毒核酸用于刺激受试者编码的蛋白质的免疫应答 通过外源核苷酸序列。