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公开(公告)号：US20160252525A1
公开(公告)日：2016-09-01
申请号：US15155912
申请日：2016-05-16
IPC分类号： G01N33/68
CPC分类号： G01N33/6872 , G01N33/4833 , G01N33/68 , G01N33/6848 , G01N33/74 , G01N2030/8831 , G01N2333/71 , G01N2333/82 , G01N2560/00 , G01N2800/52 , G01N2800/56
摘要： Specific peptides are provided, and derived ionization characteristics of those peptides, from the Hepatocyte Growth Factor Receptor (cMET) protein. The peptides are particularly and surprisingly advantageous for quantifying by the method of Selected Reaction Monitoring (SRM) mass spectrometry the cMET protein directly in biological samples that have been fixed in formalin, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed where the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including: formalin-fixed tissue/cells; formalin-fixed/paraffin embedded (FFPE) tissue/cells; FFPE tissue blocks and cells from those blocks; and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from the biological sample using the Liquid Tissue™ reagents and protocol and the cMET protein is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of a cMET peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
摘要翻译： 提供特异性肽，并从肝细胞生长因子受体（cMET）蛋白提供这些肽的衍生电离特征。 通过选择反应监测（SRM）质谱法直接在已经在福尔马林中固定的生物样品中的cMET蛋白进行定量，或者也可以称为多反应监测（MRM）质谱法，这些肽特别令人惊奇地有利 。 这种生物样品被化学保存和固定，其中生物样品选自用含甲醛的试剂/固定剂处理的组织和细胞，包括：福尔马林固定的组织/细胞; 福尔马林固定/石蜡包埋（FFPE）组织/细胞; FFPE组织块和来自这些区块的细胞; 和已被福尔马林固定和石蜡包埋的组织培养细胞。 使用Liquid Tissue TM试剂和方案从生物样品制备蛋白质样品，并且通过SRM / MRM质谱法的方法在液体组织样品中定量cMET蛋白，通过在蛋白质样品中定量至少一种或多种 描述的肽。 如果它们以修饰或未修饰的形式存在，则可以定量这些肽。 cMET肽的修饰形式的实例是肽序列内的酪氨酸，苏氨酸，丝氨酸和/或其他氨基酸残基的磷酸化。
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公开(公告)号：US20170052197A1
公开(公告)日：2017-02-23
申请号：US15307777
申请日：2015-04-30
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/743 , G01N2333/723 , G01N2800/56 , G01N2800/7028
摘要： Methods are provided for quantifying the Androgen receptor protein (AR) protein directly in biological samples that have been fixed in formalin, using Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. The biological samples are chemically preserved and fixed and can be, for example, tissues treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein sample is prepared from said biological sample using, for example, the Liquid Tissue protocol and the AR protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described.
摘要翻译： 提供了使用选择反应监测（SRM）/多重反应监测（MRM）质谱法在已经在福尔马林中固定的生物样品中直接定量雄激素受体蛋白（AR）蛋白的方法。 生物样品被化学保存和固定，并且可以是例如用含甲醛的试剂/固定剂（包括福尔马林固定的组织/细胞，福尔马林固定/石蜡包埋的（FFPE）组织/细胞，FFPE组织块和来自 那些块。 使用例如液体组织方案从所述生物样品制备蛋白质样品，并且通过SRM / MRM质谱法的方法在液体组织样品中定量AR蛋白质，通过在蛋白质样品中定量至少一种或多种 描述的肽。
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公开(公告)号：US20160313343A1
公开(公告)日：2016-10-27
申请号：US15203704
申请日：2016-07-06
CPC分类号： G01N33/6848 , A61K2039/507 , C07K16/2827 , G01N33/57423 , G01N2333/70596
摘要： The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the PD-L1 protein that are particularly advantageous for quantifying the PD-L1 protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and/or paraffin embedded. PD-L1 peptides having modified or unmodified residues can be quantitated. An example of a modification of a PD-L1 fragment peptide is a phosphorylated tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
摘要翻译： 目前的公开内容提供了来自PD-L1蛋白质的肽的特定肽和衍生的电离特征，其特别有利于通过选择反应监测方法直接在福尔马林中固定的生物样品中直接定量PD-L1蛋白 （SRM）质谱法，还可以称为多反应监测（MRM）质谱。 这样的生物样品被化学保存和固定，其中所述生物样品选自用含甲醛的试剂/固定剂（包括福尔马林固定的组织/细胞，福尔马林固定/石蜡包埋的（FFPE）组织/细胞，FFPE组织块）和 来自这些区块的细胞和已被福尔马林固定和/或石蜡包埋的组织培养细胞。 可以定量具有修饰或未修饰残基的PD-L1肽。 PD-L1片段肽的修饰的实例是肽序列内的磷酸化酪氨酸，苏氨酸，丝氨酸和/或其它氨基酸残基。
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公开(公告)号：US20150072895A1
公开(公告)日：2015-03-12
申请号：US14543610
申请日：2014-11-17
IPC分类号： G01N33/574 , G01N33/68
CPC分类号： G01N33/57492 , G01N33/57423 , G01N33/57484 , G01N33/6848 , G01N2033/57453 , G01N2333/47 , G01N2333/4704 , G01N2333/4742 , G01N2333/70596 , G01N2333/96472 , G01N2458/15 , G01N2560/00
摘要： The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins that are particularly advantageous for quantifying the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins are quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of a KRT5, KRT7, NapsinA, TTF1, TP63, and MUC1 fragment peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
摘要翻译： 目前的公开内容提供来自KRT5，KRT7，NapsinA，TTF1，TP63和/或MUC1蛋白的特定肽和衍生的电离特征，其特别有利于定量KRT5，KRT7，NapsinA，TTF1，TP63， 和/或MUC1蛋白直接在已经通过选择反应监测（SRM）质谱法的方法固定在福尔马林中的生物样品中，或者也可以称为多反应监测（MRM）质谱法。 这样的生物样品被化学保存和固定，其中所述生物样品选自用含甲醛的试剂/固定剂（包括福尔马林固定的组织/细胞，福尔马林固定/石蜡包埋的（FFPE）组织/细胞，FFPE组织块）和 来自这些区块的细胞和已被福尔马林固定和石蜡包埋的组织培养细胞。 使用Liquid Tissue TM试剂和方案从所述生物样品制备蛋白质样品，并且通过SRM / MRM质量的方法在Liquid Tissue TM样品中定量KRT5，KRT7，NapsinA，TTF1，TP63和/或MUC1蛋白质 通过在蛋白质样品中定量至少一种或多种所述的肽来进行光谱测定。 如果它们以修饰或未修饰的形式存在，则可以定量这些肽。 KRT5，KRT7，NapsinA，TTF1，TP63和MUC1片段肽的修饰形式的实例是肽序列内的酪氨酸，苏氨酸，丝氨酸和/或其他氨基酸残基的磷酸化。
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公开(公告)号：US20140206775A1
公开(公告)日：2014-07-24
申请号：US14222387
申请日：2014-03-21
IPC分类号： C12Q1/48 , G01N33/573
CPC分类号： G01N33/57484 , C12Q1/48 , G01N33/573 , G01N33/6848 , G01N2333/91051 , G01N2333/91057
摘要： Specific peptides, and derived ionization characteristics of the peptides, from the Fatty acid synthase (FASN) protein are provided that are particularly advantageous for quantifying the FASN protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed and are selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the FASN protein is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of an FASN peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
摘要翻译： 提供了来自脂肪酸合成酶（FASN）蛋白质的肽的特异性肽和衍生的电离特征，其特别有利于通过选择反应监测（SRM）的方法在福尔马林中固定的生物样品中直接定量FASN蛋白 ）质谱，或什么也可以称为多反应监测（MRM）质谱。 这些生物样品被化学保存和固定，并且选自用含甲醛的试剂/固定剂（包括福尔马林固定的组织/细胞，福尔马林固定/石蜡包埋的（FFPE）组织/细胞，FFPE组织块和来自那些的FFPE组织块和细胞）处理的组织和细胞 块和组织培养细胞，其已被福尔马林固定和石蜡包埋。 使用Liquid Tissue TM试剂和方案从所述生物样品制备蛋白质样品，并且通过SRM / MRM质谱法的方法在Liquid Tissue TM样品中定量FASN蛋白质，通过在蛋白质样品中定量至少一种或多种 描述的肽。 如果它们以修饰或未修饰的形式存在，则可以定量这些肽。 FASN肽的修饰形式的实例是肽序列内的酪氨酸，苏氨酸，丝氨酸和/或其他氨基酸残基的磷酸化。
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公开(公告)号：US20140199717A1
公开(公告)日：2014-07-17
申请号：US14223524
申请日：2014-03-24
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/6893 , G01N33/743 , G01N2333/47 , G01N2333/70567 , G01N2800/56 , G01N2800/7028
摘要： The current disclosure provides specific peptides, and derived ionization characteristics of the peptides from the estrogen receptor (ER), progesterone receptor (PR), and/or antigen Ki67 (Ki67) proteins that are particularly advantageous for quantifying the ER, PR, and/or Ki67 proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from a biological sample using the Liquid Tissue™ reagents and protocol, and the ER, PR, and/or Ki67 proteins are quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described for one or more of the ER, PR, and/or Ki67 proteins. These peptides can be quantitated if they reside in a modified or in an unmodified form. An example of a modified form of an ER, PR, and/or Ki67 peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
摘要翻译： 目前的公开内容提供特异性肽，以及来自雌激素受体（ER），孕酮受体（PR）和/或抗原Ki67（Ki67）蛋白质的肽的衍生电离特征，其特别有利于定量ER，PR和/ 或通过选择反应监测/多反应监测（SRM / MRM）质谱法的方法在已经在福尔马林中固定的生物样品中直接存在Ki67蛋白。 这些生物样品被化学保存和固定，其中生物样品选自用含甲醛的试剂/固定剂（包括福尔马林固定的组织/细胞，福尔马林固定/石蜡包埋的（FFPE）组织/细胞，FFPE组织块）和 来自这些区块的细胞和已被福尔马林固定和石蜡包埋的组织培养细胞。 使用Liquid Tissue TM试剂和方案从生物样品制备蛋白质样品，并通过SRM / MRM质谱法的方法在液体组织样品中定量分析ER，PR和/或Ki67蛋白质， 蛋白质样品至少一种或多种针对一种或多种ER，PR和/或Ki67蛋白质描述的肽。 如果它们以修饰或未修饰的形式存在，则可以定量这些肽。 ER，PR和/或Ki67肽的修饰形式的实例是肽序列内的酪氨酸，苏氨酸，丝氨酸和/或其他氨基酸残基的磷酸化。
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公开(公告)号：US20180136218A1
公开(公告)日：2018-05-17
申请号：US15676639
申请日：2017-08-14
CPC分类号： G01N33/68 , A61K38/08 , A61K38/10 , C07K7/06 , C07K7/08 , C07K14/47 , C07K16/18 , G01N33/6851 , G01N33/6887 , G01N2333/4727 , G01N2333/96433
摘要： The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the Secreted Protein Acidic and Rich in Cysteine (SPARC) protein that are particularly advantageous for quantifying the SPARC protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the SPARC protein is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of an SPARC peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
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公开(公告)号：US20160313344A1
公开(公告)日：2016-10-27
申请号：US15211425
申请日：2016-07-15
发明人： David KRIZMAN , Todd HEMBROUGH , Sheeno THYPARAMBIL , Wei-Li LIAO
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/6863 , G01N2333/70578 , G01N2800/56 , G01N2800/7028
摘要： Specific peptides, and derived ionization characteristics of those peptides from Death Receptor 5 (DRS) protein are provided that are particularly advantageous for quantifying the DRS protein directly in biological samples that have been fixed in formalin by the method ofSelected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from a biological sample using the Liquid Tissue™ reagents and protocol, and the DRS protein are quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described for one or more of the DRS protein. These peptides can be quantitated if they reside in a modified or in an unmodified form. An example of a modified form of a DRS peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence
摘要翻译： 选择反应监测/多反应监测（SRM / MRM）质谱。 这些生物样品被化学保存和固定，其中生物样品选自用含甲醛的试剂/固定剂（包括福尔马林固定的组织/细胞，福尔马林固定/石蜡包埋的（FFPE）组织/细胞，FFPE组织块）和 来自这些区块的细胞和已被福尔马林固定和石蜡包埋的组织培养细胞。 使用Liquid Tissue TM试剂和方案从生物样品制备蛋白质样品，并且通过SRM / MRM质谱法通过在蛋白质样品中定量至少一种或多种来定量在液体组织样品中的DRS蛋白质 的针对一种或多种DRS蛋白质描述的肽。 如果它们以修饰或未修饰的形式存在，则可以定量这些肽。 DRS肽的修饰形式的实例是肽序列内的酪氨酸，苏氨酸，丝氨酸和/或其它氨基酸残基的磷酸化
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公开(公告)号：US20170248608A1
公开(公告)日：2017-08-31
申请号：US15444310
申请日：2017-02-27
IPC分类号： G01N33/68 , A61K31/517 , A61K31/5377 , G01N33/574 , C07K16/28
CPC分类号： G01N33/6863 , A61K31/517 , A61K31/5377 , C07K14/71 , C07K16/2863 , C07K2317/24 , G01N33/57407 , G01N33/6842 , G01N33/6848 , G01N33/74 , G01N2333/485 , G01N2333/71 , G01N2560/00
摘要： The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the Epidermal Growth Factor Receptor (EGFR) protein that are particularly advantageous for quantifying the EGFR protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the EGFR protein is quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of an EGFR peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
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10.发明申请公开(公告)号：US20160349265A1
公开(公告)日：2016-12-01
申请号：US15236953
申请日：2016-08-15
IPC分类号： G01N33/574
CPC分类号： G01N33/57496 , G01N33/574 , G01N33/6848 , G01N2333/4703 , G01N2333/62 , G01N2333/72 , G01N2440/14 , G01N2440/38 , G01N2560/00 , G01N2800/52
摘要： The current disclosure provides for specific peptides from the Insulin Receptor Substrate 1 (IRS1) protein and the derived ionization characteristics of those peptides that are particularly advantageous for quantifying the IRS1 directly in formalin fixed biological samples by the method of Selected Reaction Monitoring (SRM) mass spectrometry. Such fixed biological samples include: formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and formalin fixed and paraffin embedded tissue culture cells. IRS1 protein is quantitated in biological samples by the method of SRM/MRM mass spectrometry by quantitating one or more of the peptides described herein. The peptides can be quantitated if they reside in a modified or an unmodified form. Examples of potentially modified forms of an IRS1 peptides include those bearing phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
摘要翻译： 目前的公开内容提供了来自胰岛素受体底物1（IRS1）蛋白的特异性肽和这些肽的衍生电离特征，其特别有利于通过选择反应监测（SRM）质量方法直接定量福尔马林固定生物样品中的IRS1 光谱测定。 这样的固定生物样品包括：福尔马林固定的组织/细胞，福尔马林固定/石蜡包埋（FFPE）组织/细胞，FFPE组织块和来自那些块的细胞，以及福尔马林固定和石蜡包埋的组织培养细胞。 通过定量本文所述的一种或多种肽，通过SRM / MRM质谱法测定生物样品中的IRS1蛋白质。 如果它们以修饰或未修饰的形式存在，则可定量肽。 IRS1肽的潜在修饰形式的实例包括在肽序列内具有酪氨酸，苏氨酸，丝氨酸和/或其他氨基酸残基磷酸化的那些。
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