公开(公告)号：US20170239658A1

公开(公告)日：2017-08-24

申请号：US15519515

申请日：2015-10-16

申请人： Ezra S. ABRAMS ,  Christian T. BOLES ,  Yu CHEN ,  James STURM ,  Robert AUSTIN ,  SAGE SCIENCE, INC. ,  TRUSTEES OF PRINCETON UNIVERSITY

发明人： Ezra S. ABRAMS ,  T. Christian BOLES ,  Yu CHEN ,  James STURM ,  Robert AUSTIN

IPC分类号： B01L3/00 ,  G01N27/447 ,  G01N15/14 ,  C12Q1/68

CPC分类号： B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2400/0421 ,  B01L2400/0487 ,  C12Q1/6806 ,  G01N15/1484 ,  G01N27/44791 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1497 ,  C12Q2523/308 ,  C12Q2565/629

摘要： In some embodiments, a method for manipulating DNA molecules for use in a microfluidic device is provided, where the method may comprise providing a solution of a plurality of DNA molecules having a first radius of gyration under under a zero flow velocity, and maintaining the DNA molecules in a spherical shape under a flow velocity.

公开(公告)号：US20210207122A1

公开(公告)日：2021-07-08

申请号：US15777577

申请日：2016-11-21

申请人： Robi David Mitra ,  Jeffrey Milbrandt ,  Ezra S. Abrams ,  Todd J. Barbera ,  T. Christian Boles ,  SAGE SCIENCE, INC. ,  WASHINGTON UNIVERSITY

发明人： Robi David MITRA ,  Jeffrey MILBRANDT ,  Ezra Solomon ABRAMS ,  Todd J. BARBERA ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447

摘要： A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.

公开(公告)号：US20170240882A1

公开(公告)日：2017-08-24

申请号：US15519516

申请日：2015-10-15

申请人： Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES ,  SAGE SCIENCE, INC.

发明人： Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447 ,  C12Q1/68

CPC分类号： C12N15/101 ,  C12M47/06 ,  C12N15/1017 ,  C12Q1/6806 ,  G01N27/44747 ,  G01N27/44773 ,  G01N27/44782

摘要： Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

公开(公告)号：US20210088473A1

公开(公告)日：2021-03-25

申请号：US16603573

申请日：2018-04-06

申请人： Sage Science, Inc.

发明人： T. Christian BOLES

IPC分类号： G01N27/447 ,  G01N27/453 ,  C12Q1/686 ,  C12Q1/6806

摘要： An electrophoresis cassette may include sample well(s), gel column(s) containing a separation gel, and elution modules arranged adjacent the gel column(s). A sample may be provided to the electrophoresis cassette and high-molecular weight DNA may be isolated from the sample. Single-copy DNA sequences may be cleaved on both sides of a repeat region of the DNA sequences to produce a cleaved sample, which then may be fractionated using gel electrophoresis. DNA fractions may be isolated from consecutive sections of the separation gel and subjected to PCR assays to detect single-copy sequences within the DNA fraction, said single-copy sequence containing repeat expansion sequences. The subjected DNA fractions may be electroeluted into the plurality of elution modules. A size of DNA fractions having the repeat expansion sequences may be determined. It is also determined if that size is above a normal repeat size range.

公开(公告)号：US20230227808A1

公开(公告)日：2023-07-20

申请号：US18149603

申请日：2023-01-03

申请人： Sage Science, Inc. ,  Washington University

发明人： Robi David Mitra ,  Jeffrey MILBRANDT ,  Ezra Solomon ABRAMS ,  Todd J. BARBERA ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447

CPC分类号： C12N15/101 ,  G01N27/44747

摘要： A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase re-agents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.

公开(公告)号：US20190153426A1

公开(公告)日：2019-05-23

申请号：US16192520

申请日：2018-11-15

申请人： SAGE SCIENCE, INC.

发明人： Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES

IPC分类号： C12N15/10 ,  G01N27/447 ,  C12Q1/6806

摘要： Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

公开(公告)号：US20230167430A1

公开(公告)日：2023-06-01

申请号：US17921049

申请日：2021-04-23

申请人： Sage Science, Inc.

发明人： T. Christian BOLES

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  G01N27/447

CPC分类号： C12N15/101 ,  C12Q1/6806 ,  G01N27/44704 ,  G01N2030/8827

摘要： Embodiments of the present disclosure present methods, systems, and devices for extrachromosomal DNA extraction, and in some embodiments, isolation of DNA therefrom, and/or analysis of the extracted and/or isolated DNA, including, in some embodiments, ecDNA.