公开(公告)号：US20170203005A1

公开(公告)日：2017-07-20

申请号：US15472855

申请日：2017-03-29

Applicant: FUJIFILM Corporation

Inventor： Reiko IWAZAWA ,  Kentaro NAKAMURA

IPC: A61L27/38 ,  A61L27/56 ,  A61L27/22 ,  A61K35/39

CPC classification number: A61L27/3804 ,  A61K9/16 ,  A61K35/12 ,  A61K35/39 ,  A61K47/42 ,  A61L27/00 ,  A61L27/222 ,  A61L27/3886 ,  A61L27/56 ,  A61L2300/64 ,  A61L2430/22

Abstract: An object of the present invention is to provide a composition containing a pancreatic islet, a cell structure containing a pancreatic islet or a pancreatic islet cell, a pancreatic islet transplantation kit, a pancreatic islet transplantation treatment agent, and a hypoglycemic agent which improve at least one of glucose sensitivity or blood sugar level-reducing performance after transplantation, and to provide a composition containing a pancreatic islet, a kit containing a pancreatic islet, a pancreatic islet cell transplantation treatment agent, and a hypoglycemic agent which can improve glucose sensitivity. According to the present invention, a composition including A: a cell structure which contains a biocompatible macromolecular block and at least one kind of cell and in which a plurality of the above-described macromolecular blocks are arranged in gaps between a plurality of the above-described cells; and B: a pancreatic islet, and a composition containing a pancreatic islet; and a spheroid formed of at least one type of stem cell are provided.

公开(公告)号：US20170100519A1

公开(公告)日：2017-04-13

申请号：US15374604

申请日：2016-12-09

Applicant: FUJIFILM Corporation

Inventor： Reiko IWAZAWA ,  Kentaro NAKAMURA

IPC: A61L27/38 ,  A61L27/56 ,  A61L27/22 ,  A61L27/50

CPC classification number: A61L27/3839 ,  A61K35/28 ,  A61L27/00 ,  A61L27/222 ,  A61L27/3808 ,  A61L27/3834 ,  A61L27/507 ,  A61L27/56 ,  A61L2430/22 ,  A61L2430/26 ,  C07K14/78

Abstract: An object of the present invention is to provide a cell structure which does not contain glutaraldehyde and can form blood vessels after transplantation, and a method for producing the above-described cell structure. According to the present invention, there is provided a cell structure which contains a biocompatible macromolecular block and at least one kind of cell and has voids and in which a plurality of the biocompatible macromolecular blocks are arranged in gaps between a plurality of the cells, in which a ratio of the volume of the biocompatible macromolecular blocks with respect to the volume of the cell structure is 10% to 30%, a ratio of the volume of the cells with respect to the volume of the cell structure is 20% to 50%, and a ratio of the volume of the voids with respect to the volume of the cell structure is 35% to 60%.

公开(公告)号：US20150352252A1

公开(公告)日：2015-12-10

申请号：US14836504

申请日：2015-08-26

Applicant: FUJIFILM CORPORATION

Inventor： Kentaro NAKAMURA ,  Reiko IWAZAWA ,  Hayato MIYOSHI ,  Kazuhiro YAMAGUCHI ,  Hideo FUSHIMI

IPC: A61L27/22 ,  A61L27/38 ,  A61L27/36

CPC classification number: A61L27/222 ,  A61L27/3604 ,  A61L27/3608 ,  A61L27/38 ,  A61L27/3834 ,  A61L27/3886 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/64 ,  A61L2430/22 ,  C12N5/0068 ,  Y10T428/2982

Abstract: It is an object of the present invention to provide a cell construct for cell transplantation that does not contain a substance having cytotoxicity, such as glutaraldehyde, and suppresses the necrosis of the transplanted cells in the construct (namely, having a high cell survival rate). The present invention provides a cell construct for cell transplantation comprising biocompatible polymer blocks that do not contain glutaraldehyde and at least one type of cells, wherein a plurality of biocompatible polymer blocks are disposed in gaps among a plurality of cells, and wherein the biocompatible polymer blocks have a tap density of 10 mg/cm3 or more and 500 mg/cm3 or less, or the value of the square root of the cross-sectional area/boundary length in the two-dimensional sectional image of the polymer block is 0.01 or more and 0.13 or less.

Abstract translation: 本发明的目的是提供一种不含有细胞毒性的物质的细胞移植用细胞构建体，例如戊二醛，抑制构建体中移植细胞的坏死（即具有高细胞存活率） 。 本发明提供了一种用于细胞移植的细胞构建体，其包含不含戊二醛和至少一种类型细胞的生物相容性聚合物嵌段，其中多个生物相容性聚合物嵌段设置在多个细胞之间的间隙中，并且其中生物相容性聚合物嵌段 具有10毫克/厘米3以上至500毫克/厘米3以下的振实密度，或聚合物嵌段的二维截面图中的截面积/边界长度的平方根的值为0.01以上 和0.13以下。

公开(公告)号：US20160303282A1

公开(公告)日：2016-10-20

申请号：US15080827

申请日：2016-03-25

Applicant: FUJIFILM Corporation

Inventor： Kentaro NAKAMURA ,  Reiko IWAZAWA ,  Kazuhiro YAMAGUCHI

IPC: A61L27/22 ,  A61L27/58 ,  F26B5/06 ,  A61L27/54 ,  A61L27/38 ,  C07K14/78 ,  A61L27/56

CPC classification number: A61L27/222 ,  A61L27/3808 ,  A61L27/3834 ,  A61L27/3886 ,  A61L27/54 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/412 ,  A61L2300/604 ,  A61L2300/64 ,  C07K14/78 ,  F26B5/06

Abstract: An object of the present invention is to provide a biocompatible macromolecular porous body, which enables provision of a cell structure showing a high number of cells and a high number of blood vessels; a method for producing the same; and a biocompatible macromolecular block and a cell structure. According to the present invention, there is provided: a method for producing a biocompatible macromolecular porous body which includes a step (a) of cooling a solution of biocompatible macromolecules to be in an unfrozen state, the difference between a temperature of a portion at the highest liquid temperature within the solution and a temperature of a portion at the lowest liquid temperature within the solution being lower than or equal to 2.5° C. and the temperature of the portion at the highest liquid temperature within the solution being lower than or equal to a melting point of a solvent, a step (b) of freezing the solution of biocompatible macromolecules obtained in the step (a), and a step (c) of freeze-drying the frozen biocompatible macromolecules obtained in the step (b); the biocompatible macromolecular porous body; a biocompatible macromolecular block; and a cell structure.

Abstract translation: 本发明的目的是提供一种生物相容性大分子多孔体，其能够提供显示大量细胞和大量血管的细胞结构; 其制造方法; 和生物相容性大分子嵌段和细胞结构。 根据本发明，提供了一种生物相容性高分子多孔体的制​​造方法，其特征在于，包括将生物体相容性大分子的溶液冷却至未冷冻状态的工序（a） 溶液中最高的液体温度，溶液中最低液温下的一部分的温度低于或等于2.5℃，溶液中最高液体温度下的部分的温度低于或等于 溶剂的熔点，步骤（b），使步骤（a）中获得的生物相容性大分子的溶液冷冻;以及步骤（c），冷冻干燥步骤（b）中得到的冷冻生物相容性大分子; 生物相容性大分子多孔体; 生物相容性大分子嵌段; 和细胞结构。

公开(公告)号：US20150202344A1

公开(公告)日：2015-07-23

申请号：US14677592

申请日：2015-04-02

Applicant: FUJIFILM Corporation

Inventor： Reiko IWAZAWA ,  Kentaro NAKAMURA

IPC: A61L27/22 ,  A61L27/36

CPC classification number: A61K9/7007 ,  A61L27/14 ,  A61L27/22 ,  A61L27/222 ,  A61L27/227 ,  A61L27/3604 ,  A61L27/3608 ,  A61L27/3625 ,  A61L27/38 ,  A61L27/3808 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/40 ,  A61L2300/64 ,  A61L2300/80 ,  A61L2400/06 ,  A61L2430/22 ,  C07K14/78

Abstract: An object of the present invention is to provide a cell construct for cell transplantation capable of having a thickness suitable for cell transplantation, preventing the necrosis of transplanted cells, and forming blood vessels in the transplantation site after transplantation. The present invention provides a cell construct for cell transplantation which comprises polymer blocks having biocompatibility and cells of at least one type, wherein the plural polymer blocks are arranged in spaces between the plural cells.

Abstract translation: 本发明的目的是提供一种能够具有适合细胞移植的厚度的细胞移植用细胞构建体，能够防止移植细胞的坏死，移植后的移植部位形成血管。 本发明提供一种用于细胞移植的细胞构建体，其包含具有生物相容性的聚合物嵌段和至少一种类型的细胞，其中所述多个聚合物嵌段排列在所述多个细胞之间的空间中。

公开(公告)号：US20140371145A1

公开(公告)日：2014-12-18

申请号：US14340942

申请日：2014-07-25

Applicant: FUJIFILM Corporation

Inventor： Reiko IWAZAWA ,  Kentaro NAKAMURA

IPC: A61K47/42 ,  A61K38/18

CPC classification number: A61K47/42 ,  A61K38/1825 ,  A61L27/222 ,  A61L27/3808 ,  A61L27/507

Abstract: A scaffold for vascular endothelial cell migration includes a recombinant gelatin having an amino acid sequence derived from a partial amino acid sequence of collagen. A method for producing a blood vessel uses this scaffold.

Abstract translation: 用于血管内皮细胞迁移的支架包括具有衍生自胶原的部分氨基酸序列的氨基酸序列的重组明胶。 血管的制造方法使用该支架。