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    Cell visual characteristic-modifying sequences
    1.
    发明申请
    Cell visual characteristic-modifying sequences 审中-公开
    细胞视觉特征修饰序列

    公开(公告)号：US20060107351A1

    公开(公告)日：2006-05-18

    申请号：US11118112

    申请日：2005-04-29

    申请人： Mirko Karan ,  Filippa Brugliera ,  John Mason ,  Sophie Dove ,  Ian Hoegh-Guldberg ,  Mark Prescott

    发明人： Mirko Karan ,  Filippa Brugliera ,  John Mason ,  Sophie Dove ,  Ian Hoegh-Guldberg ,  Mark Prescott

    IPC分类号： A01H1/00 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/415 ,  C12N1/21

    CPC分类号： C12Q1/6897 ,  A61K8/64 ,  A61K2800/86 ,  A61Q17/04 ,  C07K14/43595

    摘要： The present invention relates generally to peptides, polypeptides or proteins having one or more amino acids or one or more amino acid sequences which exhibit color-facilitating properties, either on their own or following interaction with one or more other amino acids and to nucleic acid molecules encoding same. Such peptides, polypeptides and proteins are referred to herein as “color-facilitating molecules” or “CFMs”. The present invention further provides genetic constructs for use in genetically modifying eukaryotic or prokaryotic cells and more particularly eukaryotic tissue so as to alter their visual characteristics or capacity for exhibiting same to a human eye in the absence of excitation by an extraneous non-white light or particle emission. The present invention, therefore, extends to eukaryotic or prokaryotic cells and more particularly eukaryotic tissue, which are genetically modified to produce CFMs and which thereby exhibit altered visual characteristics in the absence of excitation by an extraneous non-white light or particle emission. In one particular embodiment, the CFMs are used to alter the visual characteristics of plants and even more particularly flower color. In another embodiment, the present invention provides gels or coatings or similar biomaterials in the form of a biomatrix comprising the CFMs such as for use as a UV sink, in a sun screen, in cosmetics, as an expression marker or other reporter molecule or for use as a photon trap to increase light intensity.

    摘要翻译： 本发明一般涉及具有一个或多个氨基酸或一个或多个氨基酸序列的肽，多肽或蛋白质，其表现出颜色促进性质，其本身或之后与一种或多种其它氨基酸和核酸分子的相互作用 编码相同。 这样的肽，多肽和蛋白质在本文中称为“促色分子”或“CFM”。 本发明还提供用于遗传修饰真核细胞或原核细胞，更特别是真核组织的遗传构建体，以便在没有外来非白光的激发的情况下改变其在人眼上显示相同的视觉特征或能力 颗粒发射。 因此，本发明延伸到真核细胞或原核细胞，更特别是真核组织，其被遗传修饰以产生CFM，并且由此在没有通过外来的非白光或颗粒发射的激发的情况下表现出改变的视觉特征。 在一个具体实施方案中，CFM用于改变植物的视觉特征，甚至更特别地是花色。 在另一个实施方案中，本发明提供了生物基质形式的凝胶或涂层或类似生物材料，其包含CFM，例如用作UV吸收池，在日光屏中，在化妆品中，作为表达标记物或其他报告分子，或用于 用作光子阱以增加光强度。

    Flavanoid 3',5' hydroxylase gene sequences and uses therefor
    2.
    发明申请
    Flavanoid 3',5' hydroxylase gene sequences and uses therefor 有权
    类黄酮3'，5'羟化酶基因序列及其用途

    公开(公告)号：US20070033674A1

    公开(公告)日：2007-02-08

    申请号：US10526133

    申请日：2003-08-29

    申请人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    发明人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N9/0073 ,  C12N15/825

    摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

    摘要翻译： 本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色 花或其部分或其他植物组织。 更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。 更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。 本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。 本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。

    Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor
    3.
    发明授权
    Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor 有权

    公开(公告)号：US08445748B2

    公开(公告)日：2013-05-21

    申请号：US12566357

    申请日：2009-09-24

    申请人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    发明人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    IPC分类号： A01H5/00 ,  C12N15/63 ,  C12N5/14 ,  C07H21/00

    CPC分类号： C12N9/0073 ,  C12N15/825

    摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

    Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor
    4.
    发明授权
    Flavonoid 3′,5′ hydroxylase gene sequences and uses therefor 有权
    类黄酮3'，5'羟化酶基因序列及其用途

    公开(公告)号：US07612257B2

    公开(公告)日：2009-11-03

    申请号：US10526133

    申请日：2003-08-29

    申请人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    发明人： Filippa Brugliera ,  Yoshikazu Tanaka ,  John Mason

    IPC分类号： A01H5/00 ,  C12N15/63 ,  C12N5/14 ,  C07H21/00

    CPC分类号： C12N9/0073 ,  C12N15/825

    摘要： The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

    摘要翻译： 本发明一般涉及编码具有类黄酮3'，5'-羟化酶（F3'5'H）活性的多肽的遗传序列，以及遗传序列和/或其相应多肽的用途，尤其用于操作颜色 花或其部分或其他植物组织。 更具体地说，F3'5'H具有调节二氢卡泊芬酚（DHK）代谢以及其它底物如二氢槲皮素（DHQ），柚皮素和二碘代醇的代谢能力。 更具体地说，本发明提供编码在玫瑰或非洲菊或植物相关植物中表达时具有F3'5'H活性的多肽的遗传序列。 本发明还涉及对应于所有或部分受试者遗传序列或其转录物的反义和有义分子或RNAi诱导分子。 本发明还涉及在诸如玫瑰，非洲菊或植物相关植物的植物中有效操作的启动子。

    Dianthus plant named ‘Florijade’
    5.
    植物专利
    Dianthus plant named ‘Florijade’ 有权
    石竹植物名为“Florijade”

    公开(公告)号：USPP21515P2

    公开(公告)日：2010-11-23

    申请号：US12454905

    申请日：2009-05-26

    申请人： Filippa Brugliera

    发明人： Filippa Brugliera

    IPC分类号： A01H5/00

    CPC分类号： A01H5/02

    摘要： A new cultivar of Dianthus plant named ‘FLORIJADE’ is characterized, inter alia, by pronounced spray habit, perennial and profuse bloom, and green foliage. Dianthus ‘FLORIJADE’ is suitable for use, for example, as a flowering plant in pots, containers, window boxes, and the garden, but is primarily suited for the production of cut flowers. Dianthus ‘FLORIJADE’ is not hardy and is typically grown in a glasshouse. These traits set ‘FLORIJADE’ apart from all existing varieties, lines, strains, or sports of Dianthus. In particular, Dianthus ‘FLORIJADE’ has bright purple/violet flowers.

    摘要翻译： 名为“FLORIJADE”的石竹植物新品种的特征是特别是喷雾习性显着，多年生植物和多汁的植物和绿色的叶子。 石竹'FLORIJADE'适用于例如花盆，容器，窗框和花园中的开花植物，但主要适用于生产切花。 石竹'FLORIJADE'不硬，通常种植在温室中。 除了所有现有品种，线条，品种或运动的石竹之外，这些特征都设定了“佛罗伦萨”。 特别地，石竹'FLORIJADE'具有明亮的紫色/紫罗兰花。

    Cineraria-derived chromosomal DNA involved in synthesis of flavonoid, and use thereof
    8.
    发明授权
    Cineraria-derived chromosomal DNA involved in synthesis of flavonoid, and use thereof 有权
    涉及黄酮类化合物的花粉衍生染色体DNA及其用途

    公开(公告)号：US08852942B2

    公开(公告)日：2014-10-07

    申请号：US13124985

    申请日：2009-10-26

    申请人： Yoshikazu Tanaka ,  Filippa Brugliera

    发明人： Yoshikazu Tanaka ,  Filippa Brugliera

    IPC分类号： C12N15/00 ,  C12N5/04 ,  C12N5/10 ,  C12N1/20 ,  C07H21/04 ,  C12N9/02 ,  C12N15/82

    CPC分类号： C12N15/825 ,  C12N9/0073 ,  C12N15/8225

    摘要： Presently provided are a cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H and a F3′5′H gene construct which carries the polynucleotide. The cineraria-derived petal-specific promoter can be as polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 or a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO: 9 under stringent conditions and can act as a promoter for a coding region of flavonoid. 3′,5′-hydroxylase (F3′5′H). The gene construct can be used, for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the presently provided cineraria-derived polynucleotide.

    摘要翻译： 目前提供的是一种能够起到促进黄酮3'，5'-羟化酶（F3'5'H）和携带多核苷酸的F3'5'H基因构建体的编码区的启动子的木质素来源的多核苷酸， 衍生花瓣特异性启动子可以是具有SEQ ID NO：9所示核苷酸序列的多核苷酸或可以在严格条件下与具有SEQ ID NO：9所示核苷酸序列的多核苷酸杂交的多核苷酸，并且可以作为 3'，5'-羟化酶（F3'5'H）的编码区，该基因构建体可以用于抑制RNAi方法中的基因的表达，该RNAi方法以环作为包含 目前提供的来自牙釉质的多核苷酸的第一内含子的全部或部分。

    Dianthus plant named ‘Floriruby’
    10.
    植物专利
    Dianthus plant named ‘Floriruby’ 有权
    石竹植物名为Floriruby

    公开(公告)号：USPP23216P2

    公开(公告)日：2012-11-27

    申请号：US12930278

    申请日：2010-12-31

    申请人： Filippa Brugliera

    发明人： Filippa Brugliera

    IPC分类号： A01H5/00

    CPC分类号： A01H6/305 ,  A01H5/02

    摘要： A new and distinct cultivar of Dianthus plant named ‘Floriruby’, characterized by its upright plant habit; early and freely flowering habit; spray-type inflorescence form; and purple-colored flowers positioned upright on strong peduncles.

    摘要翻译： 一个新的和独特的石竹植物名为Floriruby，其特点是其直立的植物习性; 早期自由开花的习惯​​; 喷雾型花序形式; 紫色花朵直立在坚强的花序梗上。