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公开(公告)号：US09328385B2
公开(公告)日：2016-05-03
申请号：US14000092
申请日：2012-02-20
CPC分类号： C12Q1/6879 , C12Q1/6851 , C12Q1/6888 , C12Q2600/16 , C12Q2600/166 , C12Q2525/151 , C12Q2545/101 , C12Q2561/113
摘要： The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.
摘要翻译： 本发明涉及用于量化和/或检测样品中基因组的一种或多种核酸的方法，其中在扩增反应中，（i）第一个核酸被扩增，被扩增的基因座是多拷贝基因座 （MCL），其中所述基因座与SEQ ID NO：1的序列具有至少80％的序列同一性。 并且其中多拷贝基因座在至少两个不同染色体上具有拷贝，（ii）作为内部对照（IC）加入的第二个核酸也被扩增，并且（iii） 确定来自扩增第一核酸的扩增产物的量。
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公开(公告)号：US20140147843A1
公开(公告)日：2014-05-29
申请号：US14000092
申请日：2012-02-20
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6879 , C12Q1/6851 , C12Q1/6888 , C12Q2600/16 , C12Q2600/166 , C12Q2525/151 , C12Q2545/101 , C12Q2561/113
摘要： The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.
摘要翻译： 本发明涉及用于量化和/或检测样品中基因组的一种或多种核酸的方法，其中在扩增反应中，（i）第一个核酸被扩增，被扩增的基因座是多拷贝基因座 （MCL），其中所述基因座与SEQ ID NO：1的序列具有至少80％的序列同一性。 并且其中多拷贝基因座在至少两个不同染色体上具有拷贝，（ii）作为内部对照（IC）加入的第二个核酸也被扩增，并且（iii） 确定来自扩增第一核酸的扩增产物的量。
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公开(公告)号：US09290815B2
公开(公告)日：2016-03-22
申请号：US14000096
申请日：2012-02-21
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6851 , C12Q2600/166 , C12Q2545/113
摘要： The invention provides for a method for quantifying one or more nucleic acids of a genome in a sample comprising the steps of, (a) amplifying a first nucleic acid to be quantified, (b) determining the amount of said first nucleic acid by comparison of the amount of amplification product from said first nucleic acid with at least one amplification product from a second template nucleic acid, (c) wherein said second template nucleic acid was generated using whole genome amplification and wherein the starting concentration of the second template nucleic acid is known.
摘要翻译： 本发明提供了定量样品中一种或多种基因组核酸的方法，其包括以下步骤：（a）扩增待定量的第一核酸，（b）通过比较所述第一核酸的量来确定所述第一核酸的量 来自所述第一核酸的扩增产物与来自第二模板核酸的至少一种扩增产物的量，（c）其中所述第二模板核酸是使用全基因组扩增产生的，其中第二模板核酸的起始浓度为 已知
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公开(公告)号：US20140057263A1
公开(公告)日：2014-02-27
申请号：US14006794
申请日：2012-03-21
IPC分类号： C12Q1/68
摘要： The invention relates to a method for detecting a nucleic acid of an organism in a composition, comprising the steps of, (i) amplifying the nucleic acid to be detected, (ii) during or after amplification, hybridizing to said nucleic acid to be detected a first probe that comprises an abasic site additionally optionally carrying a detectable label, (iii) wherein the position of the abasic site corresponds to a position in said nucleic acid to be detected, known to have a polymorphism in said organism, and wherein said nucleic acid is detected if hybridization occurs.
摘要翻译： 本发明涉及一种用于检测组合物中生物体的核酸的方法，其包括以下步骤：（i）扩增要检测的核酸，（ii）在扩增期间或扩增后与待检测的核酸杂交 第一探针，其包含另外任选携带可检测标记的脱碱基位点，（iii）其中所述无碱基位点的位置对应于所述待检测核酸中的位置，已知在所述生物体中具有多态性，并且其中所述核酸 如果发生杂交，则检测到酸。
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公开(公告)号：US09150912B2
公开(公告)日：2015-10-06
申请号：US14115785
申请日：2012-05-04
CPC分类号： C12Q1/6853 , C07H21/00 , C12Q1/6813 , C12Q1/686 , C12Q1/6876 , C12Q2525/197 , C12Q2563/107 , C12Q2525/125 , C12Q2565/125 , C12Q2565/629
摘要： The present application discloses a labeled nucleotide comprising a label attached via a linker, wherein said labeled nucleotide has the formula wherein R1 is a residue with a negative net charge, preferably selected from the group consisting of a phosphate group, and a sulphate group; wherein R2, R3 and R4 are independently selected from the group consisting of H2, OH2, and O; wherein “n” is an integer between 0 and 16; wherein “a” is an integer between 1 and 10; wherein SP is absent or a spacer; wherein X is said label; and wherein Y is a nucleotide or nucleoside. Furthermore, oligonucleotides comprising a labeled nucleotide according to the present invention and the use as a primer in amplification based methods is disclosed herein.
摘要翻译： 本申请公开了一种标记的核苷酸，其包含通过连接体连接的标记，其中所述标记的核苷酸具有下式：其中R1是具有负净电荷的残基，优选选自磷酸酯基和硫酸酯基; 其中R2，R3和R4独立地选自H 2，OH 2和O; 其中“n”为0至16之间的整数; 其中“a”为1至10之间的整数; 其中SP不存在或间隔物; 其中X是所述标签; 并且其中Y是核苷酸或核苷。 此外，本文公开了包含根据本发明的标记的核苷酸的寡核苷酸和基于扩增的方法作为引物的用途。
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