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公开(公告)号:US20050004078A1
公开(公告)日:2005-01-06
申请号:US10816298
申请日:2004-04-01
IPC分类号: G01N33/53 , A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/566 , G01N33/58 , G01N37/00
CPC分类号: A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00 , Y02P20/55
摘要: The present invention is related to compounds comprising six-membered rings capable of being incorporated into nucleic acids. Particularly, the six-membered ring is a derivative of cyclohexane, cyclohexene, tetrahydropyran, tetrahydrothiopyran or piperidine. These compounds may be used to build up oligomeric compounds. The invention is further related to uses of these oligomeric compounds for hybridization and as probes. In addition, methods for the detection of nucleic acids are provided wherein the oligomeric compounds are used.
摘要翻译: 本发明涉及能够并入核酸中的包含六元环的化合物。 特别地,六元环是环己烷,环己烯,四氢吡喃,四氢噻喃或哌啶的衍生物。 这些化合物可用于构建低聚化合物。 本发明还涉及这些寡聚化合物用于杂交和作为探针的用途。 此外,提供了检测核酸的方法,其中使用低聚化合物。
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公开(公告)号:US07276592B2
公开(公告)日:2007-10-02
申请号:US10816298
申请日:2004-04-01
CPC分类号: A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00 , Y02P20/55
摘要: The present invention is related to compounds comprising six-membered rings capable of being incorporated into nucleic acids. Particularly, the six-membered ring is a derivative of cyclohexane, cyclohexene, tetrahydropyran, tetrahydrothiopyran or piperidine. These compounds may be used to build up oligomeric compounds. The invention is further related to uses of these oligomeric compounds for hybridization and as probes. In addition, methods for the detection of nucleic acids are provided wherein the oligomeric compounds are used.
摘要翻译: 本发明涉及能够并入核酸中的包含六元环的化合物。 特别地,六元环是环己烷,环己烯,四氢吡喃,四氢噻喃或哌啶的衍生物。 这些化合物可用于构建低聚化合物。 本发明还涉及这些寡聚化合物用于杂交和作为探针的用途。 此外,提供了检测核酸的方法,其中使用低聚化合物。
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公开(公告)号:US20080038745A1
公开(公告)日:2008-02-14
申请号:US11846555
申请日:2007-08-29
CPC分类号: A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00 , Y02P20/55
摘要: The present invention is related to compounds comprising six-membered rings capable of being incorporated into nucleic acids. Particularly, the six-membered ring is a derivative of cyclohexane, cyclohexene, tetrahydropyran, tetrahydrothiopyran or piperidine. These compounds may be used to build up oligomeric compounds. The invention is further related to uses of these oligomeric compounds for hybridization and as probes. In addition, methods for the detection of nucleic acids are provided wherein the oligomeric compounds are used.
摘要翻译: 本发明涉及能够并入核酸中的包含六元环的化合物。 特别地,六元环是环己烷,环己烯,四氢吡喃,四氢噻喃或哌啶的衍生物。 这些化合物可用于构建低聚化合物。 本发明还涉及这些寡聚化合物用于杂交和作为探针的用途。 此外,提供了检测核酸的方法,其中使用低聚化合物。
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公开(公告)号:US20110266172A1
公开(公告)日:2011-11-03
申请号:US13182691
申请日:2011-07-14
CPC分类号: C12N15/1006
摘要: The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译: 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上,例如具有二氧化硅表面的那些。 为此,本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸,以及用于实施这些方法的试剂盒。 特别地,本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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公开(公告)号:US20100086923A1
公开(公告)日:2010-04-08
申请号:US12480981
申请日:2009-06-09
CPC分类号: C12N15/1013 , C12N15/1003 , C12Q1/6806 , C12Q2527/125
摘要: The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译: 本发明提供了水混溶性液体环状缩醛用于将核酸吸附到固相(例如具有二氧化硅表面的基底)的用途。 为此,本发明还提供包含所述缩醛的组合物。 方法被公开并要求从样品中纯化核酸,以及用于实施这些方法的试剂盒。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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公开(公告)号:US08062843B2
公开(公告)日:2011-11-22
申请号:US12480981
申请日:2009-06-09
CPC分类号: C12N15/1013 , C12N15/1003 , C12Q1/6806 , C12Q2527/125
摘要: The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译: 本发明提供了水混溶性液体环状缩醛用于将核酸吸附到固相(例如具有二氧化硅表面的基底)的用途。 为此,本发明还提供包含所述缩醛的组合物。 方法被公开并要求从样品中纯化核酸,以及用于实施这些方法的试剂盒。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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公开(公告)号:US08124338B2
公开(公告)日:2012-02-28
申请号:US12481807
申请日:2009-06-10
CPC分类号: C12N15/1006
摘要: The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译: 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上,例如具有二氧化硅表面的那些。 为此,本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸,以及用于实施这些方法的试剂盒。 特别地,本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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公开(公告)号:US20100086924A1
公开(公告)日:2010-04-08
申请号:US12481807
申请日:2009-06-10
CPC分类号: C12N15/1006
摘要: The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译: 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上,例如具有二氧化硅表面的那些。 为此,本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸,以及用于实施这些方法的试剂盒。 特别地,本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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公开(公告)号:US08951780B2
公开(公告)日:2015-02-10
申请号:US12261476
申请日:2008-10-30
CPC分类号: B01L3/508 , B01D29/05 , B01L3/502 , B01L3/5021 , B01L2200/0631 , B01L2300/0681 , B01L2300/069
摘要: The invention concerns a method for isolating and purifying nucleic acids from large sample volumes and a device suitable for this. The device comprises a funnel, a separating column, and a tab which are detachable from each other. The method according to the invention in which the device according to the invention is used ensures a higher yield of nucleic acids.
摘要翻译: 本发明涉及从大样品体积分离和纯化核酸的方法以及适用于此的装置。 该装置包括可以彼此分离的漏斗,分离柱和突片。 根据本发明的方法,其中使用根据本发明的装置确保较高的核酸产量。
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公开(公告)号:US20100256349A1
公开(公告)日:2010-10-07
申请号:US12261476
申请日:2008-10-30
CPC分类号: B01L3/508 , B01D29/05 , B01L3/502 , B01L3/5021 , B01L2200/0631 , B01L2300/0681 , B01L2300/069
摘要: The invention concerns a method for isolating and purifying nucleic acids from large sample volumes and a device suitable for this. The device comprises a funnel, a separating column, and a tab which are detachable from each other. The method according to the invention in which the device according to the invention is used ensures a higher yield of nucleic acids.
摘要翻译: 本发明涉及从大样品体积分离和纯化核酸的方法以及适用于此的装置。 该装置包括可以彼此分离的漏斗,分离柱和突片。 根据本发明的方法,其中使用根据本发明的装置确保较高的核酸产量。
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