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    USE OF TDE FOR ISOLATION OF NUCLEIC ACIDS
    2.
    发明申请
    USE OF TDE FOR ISOLATION OF NUCLEIC ACIDS 审中-公开
    使用TDE分离核酸

    公开(公告)号：US20110266172A1

    公开(公告)日：2011-11-03

    申请号：US13182691

    申请日：2011-07-14

    申请人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    发明人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    IPC分类号： B65D71/00 ,  C12Q1/68 ,  C07H1/06

    CPC分类号： C12N15/1006

    摘要： The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

    摘要翻译： 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上，例如具有二氧化硅表面的那些。 为此，本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 特别地，本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

    USE OF ACETALS FOR ISOLATION OF NUCLEIC ACIDS
    3.
    发明申请
    USE OF ACETALS FOR ISOLATION OF NUCLEIC ACIDS 有权
    用于分离核酸的乙烯的使用

    公开(公告)号：US20100086923A1

    公开(公告)日：2010-04-08

    申请号：US12480981

    申请日：2009-06-09

    申请人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Bernd Buchberger

    发明人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Bernd Buchberger

    IPC分类号： C12Q1/68 ,  C09K3/00 ,  C07H1/00 ,  H01F1/00

    CPC分类号： C12N15/1013 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/125

    摘要： The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

    摘要翻译： 本发明提供了水混溶性液体环状缩醛用于将核酸吸附到固相（例如具有二氧化硅表面的基底）的用途。 为此，本发明还提供包含所述缩醛的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

    Use of acetals for isolation of nucleic acids
    6.
    发明授权
    Use of acetals for isolation of nucleic acids 有权
    使用缩醛分离核酸

    公开(公告)号：US08062843B2

    公开(公告)日：2011-11-22

    申请号：US12480981

    申请日：2009-06-09

    申请人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Bernd Buchberger

    发明人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Bernd Buchberger

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12N15/1013 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/125

    摘要： The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

    摘要翻译： 本发明提供了水混溶性液体环状缩醛用于将核酸吸附到固相（例如具有二氧化硅表面的基底）的用途。 为此，本发明还提供包含所述缩醛的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

    Use of TDE for isolation of nucleic acids
    8.
    发明授权
    Use of TDE for isolation of nucleic acids 有权
    使用TDE分离核酸

    公开(公告)号：US08124338B2

    公开(公告)日：2012-02-28

    申请号：US12481807

    申请日：2009-06-10

    申请人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    发明人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1006

    摘要： The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

    摘要翻译： 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上，例如具有二氧化硅表面的那些。 为此，本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 特别地，本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

    TARGET PREPARATION FOR PARALLEL SEQUENCING OF COMPLEX GENOMES
    9.
    发明申请
    TARGET PREPARATION FOR PARALLEL SEQUENCING OF COMPLEX GENOMES 审中-公开
    复杂基因并行测序的目标准备

    公开(公告)号：US20100204050A1

    公开(公告)日：2010-08-12

    申请号：US12689269

    申请日：2010-01-19

    申请人： Horst Donner ,  Bernd Buchberger

    发明人： Horst Donner ,  Bernd Buchberger

    IPC分类号： C40B20/02 ,  C12Q1/68 ,  C40B40/06

    CPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C12Q2565/501 ,  C12Q2525/191 ,  C12Q2525/155

    摘要： The present invention provides a method for the isolation and analysis of a target nucleic acid, the target nucleic acid being present in a sample of genomic DNA, comprising the steps of a) fragmentation of the genomic DNA, b) hybridization of the genomic DNA on a nucleic acid solid support, the solid support comprising a plurality of oligonucleotide probes, the probes being characterized in that each probe is at least partially complementary to the sequence of the target nucleic acid or its complement, under hybridization conditions, characterized in that the plurality of probes hybridizes to fragments of the target nucleic acid but does not hybridize to other nucleic acids which are present in the sample, c) stripping off the target molecules hybridized to the nucleic acid array, d) overlap extension synthesis in order to generate double stranded overlap extension synthesis product, e) fragment polishing, and f) adaptor ligation.

    摘要翻译： 本发明提供了分离和分析目标核酸的方法，所述靶核酸存在于基因组DNA的样品中，包括以下步骤：a）基因组DNA的断裂，b）基因组DNA的杂交 核酸固体支持物，所述固体支持物包含多个寡核苷酸探针，所述探针的特征在于，在杂交条件下，每个探针与靶核酸或其补体的序列至少部分互补，其特征在于，所述多个 的探针与靶核酸的片段杂交，但不与样品中存在的其他核酸杂交，c）剥离与核酸阵列杂交的靶分子，d）重叠延伸合成以产生双链 重叠延伸合成产物，e）碎片抛光，和f）适配器连接。

    USE OF TDE FOR ISOLATION OF NUCLEIC ACIDS
    10.
    发明申请
    USE OF TDE FOR ISOLATION OF NUCLEIC ACIDS 有权
    使用TDE分离核酸

    公开(公告)号：US20100086924A1

    公开(公告)日：2010-04-08

    申请号：US12481807

    申请日：2009-06-10

    申请人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    发明人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    IPC分类号： C12Q1/68 ,  C09K3/00 ,  C07H1/06 ,  H01F1/00

    CPC分类号： C12N15/1006

    摘要： The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

    摘要翻译： 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上，例如具有二氧化硅表面的那些。 为此，本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 特别地，本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。