公开(公告)号：US5741686A

公开(公告)日：1998-04-21

申请号：US316810

申请日：1994-10-03

申请人： Fred W. Wagner ,  Thomas R. Coolidge ,  Dwane E. Wylie ,  Sheldon M. Schuster ,  William Lewis ,  Jay Stout

发明人： Fred W. Wagner ,  Thomas R. Coolidge ,  Dwane E. Wylie ,  Sheldon M. Schuster ,  William Lewis ,  Jay Stout

IPC分类号： C07K17/06 ,  C07K1/107 ,  C07K14/575 ,  C07K16/00 ,  C07K16/40 ,  C07K17/00 ,  C07K19/00 ,  C12N11/00 ,  C12N11/02 ,  C12N11/06 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58 ,  C12N9/96 ,  C07K1/13 ,  C08H1/00

CPC分类号： C07K1/1077 ,  C07K16/40 ,  C07K17/00 ,  C12N11/00 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/815 ,  Y10S530/816

摘要： A method is provided for preparing a labeled protein, immobilized protein or protein-bioactive agent composition by attaching a label, support or bioactive agent to a protein by exopeptidase catalysis at a site that is remote from the active site of the protein. More specifically, an amine or alcohol group of an amino acid, amine or alcohol nucleophile is reacted by exopeptidase catalysis with a C-terminus carboxylic acid group of a protein such as an antibody, enzyme or hormone to couple the nucleophile to the protein to form an adduct, and the adduct is bound to an auxiliary substance such as a support, label or bioactive agent or its combination with a linker arm by reacting a reactive substituent of the nucleophile with a reactive group of the auxiliary substance. Alternatively, the nucleophile is bound to the auxiliary substance or its combination with a linker arm to form an intermediate, and the intermediate is coupled by exopeptidase catalysis to the protein. The exopeptidase may be a serine or cysteine exocarboxypeptidase.

摘要翻译： 提供了一种用于通过在远离蛋白质活性位点的位点处通过外肽酶催化将标记物，载体或生物活性剂附着到蛋白质来制备标记的蛋白质，固定的蛋白质或蛋白质 - 生物活性剂组合物的方法。 更具体地，氨基酸，胺或醇亲核试剂的胺或醇基团通过外肽酶催化与诸如抗体，酶或激素的蛋白质的C末端羧酸基团反应，以将亲核体偶联到蛋白质上形成 加合物，并且加合物通过将亲核试剂的反应性取代基与辅助物质的反应性基团反应而与辅助物质例如载体，标记或生物活性剂或其与连接臂的组合结合。 或者，亲核试剂与辅助物质或其与连接臂的组合结合以形成中间体，并且中间体通过外肽酶催化与蛋白质偶联。 外肽酶可以是丝氨酸或半胱氨酸外切羧肽酶。

公开(公告)号：US5234820A

公开(公告)日：1993-08-10

申请号：US545915

申请日：1990-06-28

申请人： Fred W. Wagner ,  Thomas R. Coolidge ,  Sheldon M. Schuster ,  Jay Stout ,  Dwane E. Wylie ,  Klaus Breddam ,  William Lewis

发明人： Fred W. Wagner ,  Thomas R. Coolidge ,  Sheldon M. Schuster ,  Jay Stout ,  Dwane E. Wylie ,  Klaus Breddam ,  William Lewis

IPC分类号： C07K1/107 ,  C07K16/40 ,  C07K17/00 ,  C12N11/00 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

CPC分类号： C12N11/00 ,  C07K1/1077 ,  C07K16/40 ,  C07K17/00 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58 ,  Y10S530/816

摘要： An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.

摘要翻译： 辅助物质如标记物，载体或生物活性剂通过使用外肽酶和亲核试剂（氨基酸，氨基酸衍生物，胺）与远离蛋白质活性位点的位点连接到蛋白质上 或酒精。 在一个实施方案中，亲核试剂通过使用外肽酶的催化与蛋白质的羧基末端连接以形成加合物，然后加合物或其与连接臂的组合与辅助物质结合。 在另一个实施方案中，辅助物质或其与连接臂的组合与亲核试剂结合以形成中间体物质，然后通过与外肽酶的催化偶联至蛋白质的羧基末端。

公开(公告)号：US5279954A

公开(公告)日：1994-01-18

申请号：US375138

申请日：1989-06-30

申请人： Fred W. Wagner ,  Thomas R. Coolidge ,  Dwane E. Wylie ,  Sheldon M. Schuster ,  William Lewis ,  Jay Stout

发明人： Fred W. Wagner ,  Thomas R. Coolidge ,  Dwane E. Wylie ,  Sheldon M. Schuster ,  William Lewis ,  Jay Stout

IPC分类号： C07K17/06 ,  C07K1/107 ,  C07K14/575 ,  C07K16/00 ,  C07K16/40 ,  C07K17/00 ,  C07K19/00 ,  C12N11/00 ,  C12N11/02 ,  C12N11/06 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58 ,  C12N11/14 ,  G01N33/544 ,  G01N33/551

CPC分类号： C07K1/1077 ,  C07K16/40 ,  C07K17/00 ,  C12N11/00 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/815 ,  Y10S530/816

摘要： The invention provides a means for attaching a label, support or bioactive agent to a protein with an exopeptidase at a site that is remote from the active site of the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amine and alcohol nucleophile to the carboxyl terminus of a protein. In one embodiment, a labeled nucleophile is attached to a protein such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein to an immobilization support and to a method for the attachment of a bioactive agent to a protein.

摘要翻译： 本发明提供了一种在远离蛋白质活性位点的位点附着标记物，载体或生物活性剂到蛋白质的方法。 更具体地，本发明涉及将氨基酸，氨基酸衍生物，胺和醇亲核试剂连接到蛋白质的羧基末端的方法。 在一个实施方案中，使用外肽酶将标记的亲核试剂连接到蛋白质例如抗体。 在其它实施方案中，本发明涉及将蛋白质与固定化载体连接的方法以及将生物活性剂与蛋白质连接的方法。

公开(公告)号：US5049656A

公开(公告)日：1991-09-17

申请号：US288009

申请日：1988-12-21

申请人： William Lewis ,  Jay Stout ,  Gino Van Heeke ,  Dwane E. Wylie ,  Sheldon M. Schuster ,  Fred W. Wagner

发明人： William Lewis ,  Jay Stout ,  Gino Van Heeke ,  Dwane E. Wylie ,  Sheldon M. Schuster ,  Fred W. Wagner

IPC分类号： A61K39/395 ,  A61K48/00 ,  C07H21/04 ,  C07K1/00 ,  C07K1/02 ,  C07K1/04 ,  C07K1/06 ,  C07K1/113 ,  C07K1/22 ,  C07K14/42 ,  C07K14/575 ,  C07K14/655 ,  C07K16/00 ,  C12N15/20

CPC分类号： C07K1/006 ,  C07K1/04 ,  C07K1/22 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/824

摘要： The invention is directed to a method for purifying sequentially synthesized peptides and oligonucleotides by immunoaffinity techniques. Selected products are lapped with an antigenic capping agent and are conjugated with antibodies that are specific for the capping agent.

摘要翻译： 本发明涉及通过免疫亲和技术纯化顺序合成的肽和寡核苷酸的方法。 选择的产物用抗原封端剂研磨，并与针对封端剂特异的抗体缀合。

公开(公告)号：US5221736A

公开(公告)日：1993-06-22

申请号：US454372

申请日：1989-12-21

申请人： Thomas R. Coolidge ,  William Lewis ,  Sheldon M. Schuster ,  Dwane Wylie ,  Fred W. Wagner ,  Jay Stout ,  Gino van Heeke

发明人： Thomas R. Coolidge ,  William Lewis ,  Sheldon M. Schuster ,  Dwane Wylie ,  Fred W. Wagner ,  Jay Stout ,  Gino van Heeke

IPC分类号： A61K39/395 ,  A61K48/00 ,  C07H21/04 ,  C07K1/00 ,  C07K1/02 ,  C07K1/04 ,  C07K1/06 ,  C07K1/113 ,  C07K1/22 ,  C07K14/42 ,  C07K14/575 ,  C07K14/655 ,  C07K16/00 ,  C12N15/20

CPC分类号： C07K1/006 ,  C07K1/04 ,  C07K1/22 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/824

摘要： The invention is directed to a method of purifying sequentially synthesized peptides and oligonucleotides by affinity techniques. Selected products are capped with and N-terminus capping agent for peptides or a 5'-terminus capping agents for oligonucleotides, and then bound with affinity agents that are selective for the corresponding capping agents.

摘要翻译： 本发明涉及通过亲和技术纯化顺序合成的肽和寡核苷酸的方法。 所选择的产物与肽封端的N-末端封端剂或寡核苷酸的5'-末端封端剂，然后与对相应的封端剂有选择性的亲和试剂结合。

公开(公告)号：US5464759A

公开(公告)日：1995-11-07

申请号：US18100

申请日：1993-02-17

申请人： Thomas R. Coolidge ,  William Lewis ,  Sheldon M. Schuster ,  Jay Stout ,  Gino van Heeke ,  Dwane Wylie ,  Fred W. Wagner

发明人： Thomas R. Coolidge ,  William Lewis ,  Sheldon M. Schuster ,  Jay Stout ,  Gino van Heeke ,  Dwane Wylie ,  Fred W. Wagner

IPC分类号： A61K39/395 ,  A61K48/00 ,  C07H21/04 ,  C07K1/00 ,  C07K1/02 ,  C07K1/04 ,  C07K1/06 ,  C07K1/113 ,  C07K1/22 ,  C07K14/42 ,  C07K14/575 ,  C07K14/655 ,  C07K16/00 ,  C12N15/20 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C07K1/006 ,  C07K1/04 ,  C07K1/22 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/824

摘要： The invention is directed to a method of purifying sequentially synthesized peptides and oligonucleotides by affinity techniques. Selected products are capped with and N-terminus capping agent for peptides or a 5'-terminus capping agents for oligonucleotides, and then bound with affinity agents that are selective for the corresponding capping agents.

摘要翻译： 本发明涉及通过亲和技术纯化顺序合成的肽和寡核苷酸的方法。 所选择的产物与肽封端的N-末端封端剂或寡核苷酸的5'-末端封端剂，然后与对相应的封端剂有选择性的亲和试剂结合。

公开(公告)号：US20060180440A1

公开(公告)日：2006-08-17

申请号：US11057004

申请日：2005-02-11

申请人： William Lewis ,  William Lewis ,  Lambert Terpstra ,  Barton Lewis

发明人： William Lewis ,  William Lewis ,  Lambert Terpstra ,  Barton Lewis

IPC分类号： B65G43/00

CPC分类号： G09F19/22

摘要： A conveyor belt device wherein a detectable coating, such as in the form of a thin film, overlies selected portions of a conveyor belt substrate surface. Suitable detectable coatings include materials that are inductive, conductive, magnetic, reflective, fluorescent and color-indicating or a combination thereof. Also disclosed are associated or corresponding conveyor belt assemblies and methods of making.

摘要翻译： 一种输送带装置，其中诸如薄膜形式的可检测涂层覆盖在输送带基材表面的选定部分上。 合适的可检测涂层包括感应，导电，磁性，反射，荧光和颜色指示的材料或其组合。 还公开了相关联或相应的传送带组件和制造方法。

公开(公告)号：US10995477B2

公开(公告)日：2021-05-04

申请号：US15910003

申请日：2018-03-01

申请人： William Lewis

发明人： William Lewis

IPC分类号： E02F9/22 ,  F16L57/00 ,  E02F3/36 ,  F16L37/56

摘要： A coupler guard configured to protect an aux coupler assembly from side impacts in the rough use environment of a loader. The coupler guard comprises an encasement and a side plate. The loader comprises the aux coupler assembly and a hinge extension. The encasement wraps around a top portion and interior side of the aux coupler assembly. The side plate attaches to a portion of the encasement and protect an exterior side portion of the aux coupler assembly. A screw assemblies selectively attaches the encasement to the side plate. A ring attaches to a portion of the side plate. The ring selectively wraps around a portion of the hinge extension of the loader. A ring screw assembly selectively holds the ring and the rest of the coupler guard to the hinge extension. An edge trim selectively sits on top of a portion of a first arm.

公开(公告)号：USD586450S1

公开(公告)日：2009-02-10

申请号：US29294995

申请日：2008-02-06

申请人： William Lewis

设计人： William Lewis

公开(公告)号：USD562952S1

公开(公告)日：2008-02-26

申请号：US29271353

申请日：2007-01-17

申请人： William Lewis

设计人： William Lewis